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Infectivity and <i>in vitro</i> development illustration.

By Nívia Carolina Nogueira-Paiva (794350), Paula Melo de Abreu Vieira (301929), Larissa Maris Rezende Oliveri (794351), Kátia da Silva Fonseca (330414), Gwenaelle Pound-Lana (794352), Maykon Tavares de Oliveira (794353), Marta de Lana (152409), Vanja Maria Veloso (152392), Alexandre Barbosa Reis (297630), Washington Luiz Tafuri (65192) and Cláudia Martins Carneiro (301932)


<p>Representative photomicrographs of <i>in vitro</i> assay infection in Vero cell cultures with Berenice-62 and Berenice-78 parental strains and subpopulations/isolates of Be-78 strain, obtained from hemocultures at 3, 6 or 12 months after infection of Swiss mice with 5000 blood trypomastigotes from Be-78 <i>Trypanosoma cruzi</i> strain. After 18 hours exposure to the parasite, the cells were washed to remove extracellular parasites and maintained in medium until collection, fixed and stained at 24, 48 or 72 hours after inoculation. Increasing the rate of infectivity (asterisks) 48h after incubation for Be-62 strain (a, b, c) strain and for Be 78is5-3mai isolate (g, h, i); Increased rate of intracellular multiplication (asterisks) in 72h time for Be-78 parental strain (d, e, f); Infectivity and intracellular development profiles upper (asterisks) for isolate Be-78is2-6mai (j, k. G), and lower for Be-78is14-12mai (m, n, o). Fast Panotic. Bar = 25μm.</p

Topics: Uncategorised, dna, trypanosoma cruzi, Berenice, polyclonal strain, patient 16 years, Vero cell medium, inoculating strain, Genetically Unidentical Isolates, Single Trypanosoma cruzi Strain Identified, polyclonal Trypanosoma cruzi strain
Year: 2015
DOI identifier: 10.1371/journal.pone.0137788.g003
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Provided by: FigShare
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