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Measurement of cellular impedance in FlpIn-TREx-HEK293-mGPRC6A cells.

By Patricia Rueda (35738), Elizabeth Harley (846442), Yao Lu (185912), Gregory D. Stewart (846443), Stewart Fabb (846444), Natalie Diepenhorst (846445), Béatrice Cremers (846446), Marie-Hélène Rouillon (846447), Isabelle Wehrle (846448), Anne Géant (264147), Gwladys Lamarche (846449), Katie Leach (491058), William N. Charman (846450), Arthur Christopoulos (538751), Roger J. Summers (437267), Patrick M. Sexton (538750) and Christopher J. Langmead (232083)

Abstract

<p>(A) ATP (acting via endogenous P2Y receptors) increases cellular impedance in both tetracycline-induced and un-induced FlpIn-TREx-HEK293-mGPRC6A cells, whereas (B) L-ornithine increases cellular impedance only in tetracycline-induced cells. (C) Bovine OCN did not change cell impedance in either induced or un-induced FlpIn-TREx-HEK293-mGPRC6A cells. A similar lack of effect was shown for other OCN variants (<i>data not shown</i>).</p

Topics: Biological Sciences, Gprc 6a KO mice, ocn, class C G protein, Murine GPRC 6A Mediates Cellular Responses, Osteocalcin Variants Phenotyping, GPRC 6A cells, mouse GPRC 6A, glp, murine GPRC 6A receptor, insulin release assays, Gprc 6a
Year: 2016
DOI identifier: 10.1371/journal.pone.0146846.g004
OAI identifier: oai:figshare.com:article/1638881
Provided by: FigShare
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