CD14 interacts with and is essential for the functions of endosomal TLR7 and TLR9 in mice

Akashi-Takamura

Andreas Pichlmair

Andrejeva

Blasius

Bouwmeester

Brinkmann

Bürckstümmer

Christoph L. Baumann

Diebold

Dixit

Dunzendorfer

Fitzgerald

Florian Grebien

Gavin

Giulio Superti-Furga

Habjan

Häcker

Irene M. Aspalter

Jacques Colinge

Karin Aumayr

Kato

Kawasaki

Keiryn L. Bennett

Krieg

Köcher

Lande

Latz

Lund

Manuela Bruckner

Medzhitov

Melanie Planyavsky

Moore

Omar Sharif

O’Neill

Park

Pawel Pasierbek

Pugin

Rappsilber

Roberts

Rogers

Schmitz

Shamsul

Shevchenko

Soulat

Stephan Blüml

Stetson

Stojdl

Sylvia Knapp

Takahashi

Takeda

Takeuchi

Tian

Ulevitch

von Schlieffen

Wengner

Yoneyama

Zanoni

English

PubMed

Recognition of pathogens by the innate immune system requires proteins that detect conserved molecular patterns. Nucleic acids are recognized by cytoplasmic sensors as well as by endosomal Toll-like receptors (TLRs). It has become evident that TLRs require additional proteins to be activated by their respective ligands. In this study, we show that CD14 (cluster of differentiation 14) constitutively interacts with the MyD88-dependent TLR7 and TLR9. CD14 was necessary for TLR7- and TLR9-dependent induction of proinflammatory cytokines in vitro and for TLR9-dependent innate immune responses in mice. CD14 associated with TLR9 stimulatory DNA in precipitation experiments and confocal imaging. The absence of CD14 led to reduced nucleic acid uptake in macrophages. Additionally, CD14 played a role in the stimulation of TLRs by viruses. Using various types of vesicular stomatitis virus, we showed that CD14 is dispensable for viral uptake but is required for the triggering of TLR-dependent cytokine responses. These data show that CD14 has a dual role in nucleic acid–mediated TLR activation: it promotes the selective uptake of nucleic acids, and it acts as a coreceptor for endosomal TLR activation.</jats:p

Crossref

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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2989773

CD14 is a coreceptor of Toll-like receptors 7 and 9

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