3 research outputs found
Beneficial Bacteria Isolated from Grapevine Inner Tissues Shape Arabidopsis thaliana Roots
We investigated the potential plant growth-promoting traits of 377 culturable endophytic bacteria, isolated from Vitis vinifera cv. Glera, as good biofertilizer candidates in vineyard management. Endophyte ability in promoting plant growth was assessed in vitro by testing ammonia production, phosphate solubilization, indole-3-acetic acid (IAA) and IAA-like molecule biosynthesis, siderophore and lytic enzyme secretion. Many of the isolates were able to mobilize phosphate (33%), release ammonium (39%), secrete siderophores (38%) and a limited part of them synthetized IAA and IAA-like molecules (5%). Effects of each of the 377 grapevine beneficial bacteria on Arabidopsis thaliana root development were also analyzed to discern plant growth-promoting abilities (PGP) of the different strains, that often exhibit more than one PGP trait. A supervised model-based clustering analysis highlighted six different classes of PGP effects on root architecture. A. thaliana DR5::GUS plantlets, inoculated with IAA-producing endophytes, resulted in altered root growth and enhanced auxin response. Overall, the results indicate that the Glera PGP endospheric culturable microbiome could contribute, by structural root changes, to obtain water and nutrients increasing plant adaptation and survival. From the complete cultivable collection, twelve promising endophytes mainly belonging to the Bacillus but also to Micrococcus and Pantoea genera, were selected for further investigations in the grapevine host plants towards future application in sustainable management of vineyards
Suitability of litter amendments for the Australian chicken meat industry
This project focused on litter amendment products, which are used overseas during the rearing of meat chickens. Litter amendments are primarily used to manage ammonia volatilisation, especially when litter is reused, but also provide antimicrobial and environmental benefits, and increase the nutrient value of spent litter.
This report summarises the outcomes of consultation with representatives and stakeholders of the Australian chicken meat industry, and summarises key findings from a literature review on litter amendments
Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45
Not AvailableProduction of exopolysaccharides (EPS) can be
used as a criteria for the isolation of stress tolerant
microorganisms. In the present study, EPS-producing
fluorescent pseudomonads were isolated from alfisols,
vertisols, inseptisols, oxisols, and aridisols of different
semiarid millet growing regions of India and were screened
in vitro for drought tolerance in trypticase soy broth
supplemented with different concentrations of polyethylene
glycol (PEG6000). Out of the total 81 isolates, 26 could
tolerate maximum level of stress (−0.73 MPa) and were
monitored for the amount of EPS produced under maximum level of water stress. The strain GAP-P45, isolated
from alfisol of sunflower rhizosphere, showed the highest
level of EPS production under water stress conditions, was
identified as Pseudomonas putida on the basis of 16S
rDNA sequence analysis, and was used as seed treatment to
study its effect in alleviating drought stress effects in
sunflower seedlings. Inoculation of Pseudomonas sp. strain
GAP-P45 increased the survival, plant biomass, and root
adhering soil/root tissue ratio of sunflower seedlings
subjected to drought stress. The inoculated bacteria could
efficiently colonize the root adhering soil and rhizoplane
and increase the percentage of stable soil aggregates.
Scanning electron microscope studies showed the formation
of biofilm of inoculated bacteria on the root surface and
this, along with a better soil structure, might have protected
the plants from the water stressNot Availabl