Assessing Potential Impact of a Farmer Field School Training on Perennial Crop in Cameroon

This study is an attempt of the combination of multiple data sources referring to the same time period and to the same farmer population, it aims at assessing the potential impact of a cocoa Farmer Field School Training on Integrated Pest Management in Cameroon. Using a combination of a latitudinal and a longitudinal comparison, the results indicate that FFS-trained farmers have significantly more knowledge about crop husbandry practices than those in the non-participant comparison group. A 32% production increase and 45% income increase relative to the non-participants was estimated in the latitudinal analysis. The longitudinal comparison is showing significant adoption rates of 94, 93, 90, 66 and 35 % respectively for shade management, phytosanitary harvest, pruning, improved spraying practices and grafting of improved materials. There was a 47 % reduction in the frequency of spraying fungicides and a 17 % reduction in the number of sprayers applied per treatment following the implementation of the training. Labour inputs increased significantly for pruning, phytosanitary harvest, and shade management but decreased for spraying. A partial budget analysis reveals that the IPM practices lowered overall costs of production by 11 % relative to previous practices. The two different analytical tools (longitudinal and latitudinal) are convergent in their results, showing more evidence about the higher potential impact of the farmer field school training on the restructuring process of the cocoa sector in Cameroonintegrated pest management, farmer field school, adoption rate, Agricultural and Food Policy, Community/Rural/Urban Development, Demand and Price Analysis, Environmental Economics and Policy, Farm Management, Food Consumption/Nutrition/Food Safety, Food Security and Poverty, Land Economics/Use, Marketing, Research and Development/Tech Change/Emerging Technologies,

Mach number distribution on blade to blade surface of a turbine stator pasage

Mach number distribution on the blade to blade surface l was computed at the hub, mean and tip sections of a stator' blade using the computer program COMPBLADE. These results,; were used to plot i so-Mach contours on the blade to blade surface and surface velocity distribution as a function- of fractional surface length. The results have been presented`' in this report

Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size

Modified vaccinia virus Ankara (MVA) is a candidate vaccine vector that is severely attenuated due to mutations acquired during several hundred rounds of serial passage in vitro. A previous study used marker rescue to produce a set of MVA recombinants with improved replication on mammalian cells. Here, we extended the characterization of these rescued MVA strains and identified vaccinia virus (VACV) gene F5L as a determinant of plaque morphology but not replication in vitro. F5 joins a growing group of VACV proteins that influence plaque formation more strongly than virus replication and which are disrupted in MVA. These defective genes in MVA confound the interpretation of marker rescue experiments designed to map mutations responsible for the attenuation of this important VACV strain.This work was funded by grants to DCT: NHMRC APP1023141 and ARC FT110100310

The development of a mathematical programming technique as a design tool for traffic management

In urban areas, competition for road space at junctions is one of the major causes of congestion and accidents. Routes chosen to avoid conflict at junctions have a mutually beneficial effect which should improve circulation and reduce accidents. A prototype design tool has been developed to provide for traffic management based on such routes. The mathematical model behind the design tool works with a given road network and a given O-D demand matrix to produce feasible routes for all drivers in such a way that the weighted sum of potential conflicts is minimised. The result is a route selection in which all journeys from origin i to destination j follow the same route. The method which works best splits the problem into single commodity problems and solves these repeatedly by the Out-of-Kilter algorithm. Good locally optimal solutions can be produced by this method, even though global optimality cannot be guaranteed. Software for a microcomputer presented here as part of the design tool is capable of solving problems on realistic networks in a reasonable time. This method is embedded in a suite of computer programs which makes the input and output straightforward. Used as a design tool in the early stages of network design it gives a network-wide view of the possibilities for reducing conflict and indicates a coherent set of traffic management measures. The ideal measure would be automatic route guidance, such as the pilot scheme currently being developed for London. Other measures include a set of one-way streets and banned turns. The resulting turning flows could be used as input to the signal optimiser TRANSYT to determine signal settings favouring the routeing pattern. The project was funded by the S. E. R. C. and carried out at Middlesex Polytechnic in collaboration with MVA Systematica

Immunogenicity and safety of three consecutive production lots of the non replicating smallpox vaccine MVA: A randomised, double blind, placebo controlled phase III trial

<div><p>Background</p><p>Modified Vaccinia Ankara (MVA) is a live, viral vaccine under advanced development as a non-replicating smallpox vaccine. A randomised, double-blind, placebo-controlled phase III clinical trial was conducted to demonstrate the humoral immunogenic equivalence of three consecutively manufactured MVA production lots, and to confirm the safety and tolerability of MVA focusing on cardiac readouts.</p><p>Methods</p><p>The trial was conducted at 34 sites in the US. Vaccinia-naïve adults aged 18-40 years were randomly allocated to one of four groups using a 1:1:1:1 randomization scheme. Subjects received either two MVA injections from three consecutive lots (Groups 1-3), or two placebo injections (Group 4), four weeks apart. Everyone except personnel involved in vaccine handling and administration was blinded to treatment. Safety assessment focused on cardiac monitoring throughout the trial. Vaccinia-specific antibody titers were measured using a Plaque Reduction Neutralization Test (PRNT) and an Enzyme-Linked Immunosorbent Assay (ELISA). The primary immunogenicity endpoint was Geometric Mean Titers (GMTs) after two MVA vaccinations measured by PRNT at trial visit 4. This trial is registered with ClinicalTrials.gov, number NCT01144637.</p><p>Results</p><p>Between March 2013 and May 2014, 4005 subjects were enrolled and received at least one injection of MVA (n = 3003) or placebo (n = 1002). The three MVA lots induced equivalent antibody titers two weeks after the second vaccination, with seroconversion rates of 99·8% (PRNT) and 99·7% (ELISA). Overall, 180 (6·0%) subjects receiving MVA and 29 (2·9%) subjects in the placebo group reported at least one unsolicited Adverse Event (AE) that was considered trial-related. Vaccination was well tolerated without significant safety concerns, particularly regarding cardiac assessment.</p><p>Conclusions</p><p>The neutralizing and total antibody titers induced by each of the three lots were equivalent. No significant safety concerns emerged in this healthy trial population, especially regarding cardiac safety, thus confirming the excellent safety and tolerability profile of MVA.</p><p>Trial registration</p><p>ClinicalTrials.gov <a href="https://clinicaltrials.gov/ct2/show/NCT01144637" target="_blank">NCT01144637</a></p></div

A comparison of recently introduced instruments for measuring rice flour viscosity

The Rapid Visco-Analyser (RVA) and the Micro Visco-Amylograph (MVA) were compared in measuring the viscosity properties of rice flours. A total of 72 rice samples were procured from three cultivars harvested at two locations and three moisture contents and separated into thin, medium, and thick kernel-thickness fractions. A fast and a slow heating rate was used in the procedure for both instruments. Cultivar, kernel thickness, and harvest location affected rice viscosity. The RVA viscosity profiles using a fast heating rate were best correlated with those of the MVA using a slow heating rate. The RVA slow heating rate resulted in lower final viscosities than those using the MVA because of the spindle structure of the RVA. For both the RVA and the MVA, greater rice flour peak viscosities and less trough and final viscosities were obtained with a slow rather than a fast heating rat

Vaccination directed against the human endogenous retrovirus-K envelope protein inhibits tumor growth in a murine model system

Human endogenous retrovirus (HERV) genomes are chromosomally integrated in all cells of an individual. They are normally transcriptionally silenced and transmitted only vertically. Enhanced expression of HERV-K accompanied by the emergence of anti-HERV-K-directed immune responses has been observed in tumor patients and HIV-infected individuals. As HERV-K is usually not expressed and immunological tolerance development is unlikely, it is an appropriate target for the development of immunotherapies. We generated a recombinant vaccinia virus (MVA-HKenv) expressing the HERV-K envelope glycoprotein (ENV), based on the modified vaccinia virus Ankara (MVA), and established an animal model to test its vaccination efficacy. Murine renal carcinoma cells (Renca) were genetically altered to express E. coli beta-galactosidase (RLZ cells) or the HERV-K ENV gene (RLZ-HKenv cells). Intravenous injection of RLZ-HKenv cells into syngenic BALB/c mice led to the formation of pulmonary metastases, which were detectable by X-gal staining. A single vaccination of tumor-bearing mice with MVA-HKenv drastically reduced the number of pulmonary RLZ-HKenv tumor nodules compared to vaccination with wild-type MVA. Prophylactic vaccination of mice with MVA-HKenv precluded the formation of RLZ-HKenv tumor nodules, whereas wild-type MVA-vaccinated animals succumbed to metastasis. Protection from tumor formation correlated with enhanced HERV-K ENV-specific killing activity of splenocytes. These data demonstrate for the first time that HERV-K ENV is a useful target for vaccine development and might offer new treatment opportunities for diverse types of cancer

The MVA Priority Approximation

A Mean Value Analysis (MVA) approximation is presented for computing the average performance measures of closed-, open-, and mixed-type multiclass queuing networks containing Preemptive Resume (PR) and nonpreemptive Head-Of-Line (HOL) priority service centers. The approximation has essentially the same storage and computational requirements as MVA, thus allowing computationally efficient solutions of large priority queuing networks. The accuracy of the MVA approximation is systematically investigated and presented. It is shown that the approximation can compute the average performance measures of priority networks to within an accuracy of 5 percent for a large range of network parameter values. Accuracy of the method is shown to be superior to that of Sevcik's shadow approximation

Does limited virucidal activity of biocides include duck hepatitis B virucidal action?

BACKGROUND: There is agreement that the infectivity assay with the duck hepatitis B virus (DHBV) is a suitable surrogate test to validate disinfectants for hepatitis B virucidal activity. However, since this test is not widely used, information is necessary whether disinfectants with limited virucidal activity also inactivate DHBV. In general, disinfectants with limited virucidal activity are used for skin and sensitive surfaces while agents with full activity are more aggressive. The present study compares the activity of five different biocides against DHBV and the classical test virus for limited virucidal activity, the vaccinia virus strain Lister Elstree (VACV) or the modified vaccinia Ankara strain (MVA). METHODS: Virucidal assay was performed as suspension test according to the German DVV/RKI guideline. Duck hepatitis B virus obtained from congenitally infected Peking ducks was propagated in primary duck embryonic hepatocytes and was detected by indirect immunofluorescent antigen staining. RESULTS: The DHBV was inactivated by the use of 40% ethanol within 1-min and 30% isopropanol within 2-min exposure. In comparison, 40% ethanol within 2-min and 40% isopropanol within 1-min exposure were effective against VACV/MVA. These alcohols only have limited virucidal activity, while the following agents have full activity. 0.01% peracetic acid inactivated DHBV within 2 min and a concentration of 0.005% had virucidal efficacy against VACV/MVA within 1 min. After 2-min exposure, 0.05% glutardialdehyde showed a comparable activity against DHBV and VACV/MVA. This is also the case for 0.7% formaldehyde after a contact time of 30 min. CONCLUSIONS: Duck hepatitis B virus is at least as sensitive to limited virucidal activity as VACV/MVA. Peracetic acid is less effective against DHBV, while the alcohols are less effective against VACV/MVA. It can be expected that in absence of more direct tests the results may be extrapolated to HBV