40,620 research outputs found
Flagella bending affects macroscopic properties of bacterial suspensions
To survive in harsh conditions, motile bacteria swim in complex environment
and respond to the surrounding flow. Here we develop a PDE model describing how
the flagella bending affects macroscopic properties of bacterial suspensions.
First, we show how the flagella bending contributes to the decrease of the
effective viscosity observed in dilute suspension. Our results do not impose
tumbling (random re-orientation) as it was done previously to explain the
viscosity reduction. Second, we demonstrate a possibility of bacterium escape
from the wall entrapment due to the self-induced buckling of flagella. Our
results shed light on the role of flexible bacterial flagella in interactions
of bacteria with shear flow and walls or obstacles
Movement of the Flagella of Polytoma Uvella
1. Dark-field photographic records of the wave patterns of moving flagella have been made using multiple-flash exposures at flash rates of up to 50 per sec. Patterns obtained from ATP-reactivated isolated flagella show reduced amplitude of bending, but are otherwise similar to those obtained from flagella under normal conditions. The co-ordination required to produce propagated waves of active bending appears to be preserved after isolation and reactivation.
2. Addition of methyl cellulose to the medium to increase the viscosity reduces the frequency of beat much more than the amplitude. This behaviour can be partially explained by an analysis of the equations for sinusoidal wave movement of flagella which shows that maximum efficiency of forward swimming will be obtained if the amplitude of beat is maintained greater than 1/2{pi} times the wavelength, and variations in available power or viscosity are compensated by changes in beat frequency.
3. Wave patterns at low frequencies in low ATP concentrations are unlike those obtained when the frequency is reduced by increased viscosity. The effect of ATP concentration on beat frequency is not explained by an effect on the power available for beating or by an effect on the 'internal viscosity' of the flagella
The \u3cem\u3eChlamydomonas\u3c/em\u3e Genome Reveals the Evolution of Key Animal and Plant Functions
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella
General and Specific Promotion of Flagellar Assembly by a Flagellar Nucleoside Diphosphate Kinase
Nucleoside diphosphate kinases (NDKs) play a central role in diverse cellular processes using the canonical NDK activity or alternative mechanisms that remain poorly defined. Our study of dimeric NDK5 in a flagellar motility control complex, the radial spoke (RS), has revealed new modalities. The flagella in Chlamydomonas ndk5 mutant were paralyzed, albeit only deficient in three RS subunits. RS morphology appeared severely changed in averaged cryo-electron tomograms, suggesting that NDK5 is crucial for the intact spokehead formation as well as RS structural stability. Intriguingly, ndk5’s flagella were also short, resembling those of an allelic spoke-less mutant. All ndk5’s phenotypes were rescued by expressions of NDK5 or a mutated NDK5 lacking the canonical kinase activity. Importantly, the mutated NDK5 that appeared fully functional in ndk5 cells elicited a dominant-negative effect in wild-type cells, causing paralyzed short flagella with hypophosphorylated, less abundant, but intact RSs, and accumulated hypophosphorylated NDK5 in the cell body. We propose that NDK5 dimer is an RS structural subunit with an additional mechanism that uses cross-talk between the two NDK monomers to accelerate phosphorylation-related assembly of RSs and entire flagella
Beating patterns of filaments in viscoelastic fluids
Many swimming microorganisms, such as bacteria and sperm, use flexible
flagella to move through viscoelastic media in their natural environments. In
this paper we address the effects a viscoelastic fluid has on the motion and
beating patterns of elastic filaments. We treat both a passive filament which
is actuated at one end, and an active filament with bending forces arising from
internal motors distributed along its length. We describe how viscoelasticity
modifies the hydrodynamic forces exerted on the filaments, and how these
modified forces affect the beating patterns. We show how high viscosity of
purely viscous or viscoelastic solutions can lead to the experimentally
observed beating patterns of sperm flagella, in which motion is concentrated at
the distal end of the flagella
Swarm behavior of self-propelled rods and swimming flagella
Systems of self-propelled particles are known for their tendency to aggregate
and to display swarm behavior. We investigate two model systems, self-propelled
rods interacting via volume exclusion, and sinusoidally-beating flagella
embedded in a fluid with hydrodynamic interactions. In the flagella system,
beating frequencies are Gaussian distributed with a non-zero average. These
systems are studied by Brownian-dynamics simulations and by mesoscale
hydrodynamics simulations, respectively. The clustering behavior is analyzed as
the particle density and the environmental or internal noise are varied. By
distinguishing three types of cluster-size probability density functions, we
obtain a phase diagram of different swarm behaviors. The properties of
clusters, such as their configuration, lifetime and average size are analyzed.
We find that the swarm behavior of the two systems, characterized by several
effective power laws, is very similar. However, a more careful analysis reveals
several differences. Clusters of self-propelled rods form due to partially
blocked forward motion, and are therefore typically wedge-shaped. At higher rod
density and low noise, a giant mobile cluster appears, in which most rods are
mostly oriented towards the center. In contrast, flagella become
hydrodynamically synchronized and attract each other; their clusters are
therefore more elongated. Furthermore, the lifetime of flagella clusters decays
more quickly with cluster size than of rod clusters
Modelling the Mechanics and Hydrodynamics of Swimming E. coli
The swimming properties of an E. coli-type model bacterium are investigated
by mesoscale hy- drodynamic simulations, combining molecular dynamics
simulations of the bacterium with the multiparticle particle collision dynamics
method for the embedding fluid. The bacterium is com- posed of a
spherocylindrical body with attached helical flagella, built up from discrete
particles for an efficient coupling with the fluid. We measure the hydrodynamic
friction coefficients of the bacterium and find quantitative agreement with
experimental results of swimming E. coli. The flow field of the bacterium shows
a force-dipole-like pattern in the swimming plane and two vor- tices
perpendicular to its swimming direction arising from counterrotation of the
cell body and the flagella. By comparison with the flow field of a force dipole
and rotlet dipole, we extract the force- dipole and rotlet-dipole strengths for
the bacterium and find that counterrotation of the cell body and the flagella
is essential for describing the near-field hydrodynamics of the bacterium
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