2 research outputs found
An alternative pathway for ureide usage in legumes: enzymatic formation of a ureidoglycolate adduct in Cicer arietinum and Phaseolus vulgaris
12 páginas, 7 figuras, 2 tablas.-- El documento en word es la versión post-print.-- et al.Ureidoglycolate is an intermediate in the degradation of the ureides, allantoin and allantoate, found in many
organisms. In some leguminous plant species these compounds are used to transport recently fixed nitrogen in the
root nodules to the aerial parts of the plant. In the present study, it was demonstrated that purified ureidoglycolases
from chickpea (Cicer arietinum) and French bean (Phaseolus vulgaris) do not produce glyoxylate, and can use
phenylhydrazine as a substrate with Km values of 4.0 mM and 8.5 mM, respectively. Furthermore, these enzymes
catalyse the transfer of the ureidoglycolyl group to phenylhydrazine to produce ureidoglycolyl phenylhydrazide,
which degrades non-enzymatically to glyoxylate phenylhydrazone and urea. This supports their former classification
as ureidoglycolate urea-lyases. The enzymatic reaction catalysed by the characterized ureidoglycolases uncovered
here can be viewed as a novel type of phenylhydrazine ureidoglycolyl transferase. The implications of these findings
for ureide metabolism in legume nitrogen metabolism are discussed.This work was supported by the Ministerio de Ciencia e
Innovación (AGL2009-11290), ConsejerÃa de Innovación,
Ciencia y Empresa de la Junta de AndalucÃa (CVI1761),
and Plan Andaluz de Investigación (CVI-115). G Bannenberg is a Ramón
y Cajal fellow supported by the Centro Nacional de
BiotecnologÃa/CSIC and the Ministry of Science and
Innovation, Spain.Peer reviewe