Active properties of edible marine polysaccharide-based coatings containing Larrea nitida polyphenols enriched extract

The aim of this work was to develop active edible coatings based on marine polysaccharide matrices and polyphenols-enriched native plant extracts from arid and semiarid regions of Argentina. Initially, five plant extracts were characterized in terms of antioxidant, antibacterial and antiviral activity and the one with better biological properties and no toxicity or genotoxicity, Larrea nitida (Ln) extract, was incorporated into agar, alginate or agar/alginate matrices. The Ln extract-containing films were characterized in terms of physicochemical and polyphenols release performance in food simulants. The incorporation of Ln extract provided darker films, with a more saturated orange-brownish color and with negligible effects on mechanical and barrier properties. Additionally, all the coatings showed antiviral activity when applied to blueberries against murine norovirus (MNV), a cultivable norovirus surrogate. The coatings of agar and Ln extract was able to reduce the infectivity of MNV below the limit of detection after over-night (ON) incubation at 25 °C and after 4 days at 10 °C storage. These edible polysaccharides coatings containing Ln extract could be an alternative to reduce or eliminate foodborne viruses and protect the food against oxidative process.This research was supported by grants from Secretaría de Ciencia, Arte e Innovación Tecnológica de la Universidad Nacional de Tucumán, Argentina (PIUNT 637), Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina (Proyecto UE-0011, doctoral and postdoctoral scholarship), Agencia Nacional de Promoción Científica y Tecnológica (PICT 3136, PICT 4436), Agencia Estatal de Investigación (AEI, Grant PCI2018-092886) and cofunded by the European Union's Horizon 2020 research and innovation programme (ERA-Net SUSFOOD2), and EMHE-CSIC (Grant MHE-200038). MJF was supported by a Ramon y Cajal contract (RYC2014-158) from the Spanish Ministerio de Economia; Industria y Competitividad. The authors thank the Central Support Service for Experimental Research (SCSIE) of the University of Valencia for the electronic microscopy service.Peer reviewe

Active properties of edible marine polysaccharide-based coatings containing Larrea nitida polyphenols enriched extract

The aim of this work was to develop active edible coatings based on marine polysaccharide matrices and polyphenols-enriched native plant extracts from arid and semiarid regions of Argentina. Initially, five plant extracts were characterized in terms of antioxidant, antibacterial and antiviral activity and the one with better biological properties and no toxicity or genotoxicity, Larrea nitida (Ln) extract, was incorporated into agar, alginate or agar/alginate matrices. The Ln extract-containing films were characterized in terms of physicochemical and polyphenols release performance in food simulants. The incorporation of Ln extract provided darker films, with a more saturated orange-brownish color and with negligible effects on mechanical and barrier properties. Additionally, all the coatings showed antiviral activity when applied to blueberries against murine norovirus (MNV), a cultivable norovirus surrogate. The coatings of agar and Ln extract was able to reduce the infectivity of MNV below the limit of detection after over-night (ON) incubation at 25 °C and after 4 days at 10 °C storage. These edible polysaccharides coatings containing Ln extract could be an alternative to reduce or eliminate foodborne viruses and protect the food against oxidative process.Fil: Moreno, María Alejandra. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Bojorges, Hylenne. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Falcó, Irene. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Sánchez, Gloria. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: López Carballo, Gracia. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Lopez Rubio, Amparo. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Fabra, María José. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; Españ