Identification and characterisation of the circadian pacemaker of the cockroach Leucophaea maderae

Identification and characterisation of the circadian pacemaker of the cockroach Leucophaea madera

Geometry Morphometry and Health Status of Hawksbill Turtle (Eretmochelys imbricata Linnaeus, 1766) in Maratua Island, East Kalimantan-Indonesia

Research on hawksbill turtles in the Maratua Island is still very few. Meanwhile, population of hawksbills was believed to decline due to human exploitation. The aim of this research was to study the morphology of hawksbill through geometric morphometric approach and health status which includes the existence of epibionts, mechanical damages and Body Condition Index. The survey was conducted from 5 to 18 September 2014. The method used was the hand˗catching by snorkeling and diving for catching turtles, morphometric measurements and documentation of the turtle body parts using the camera. Morphological aspects were analyzed with linear regression and geometry approach with Microsoft Excel and ImageJ software. Health status was analyzed with observation of epibionts and mechanical damages through photos and calculation of Body Condition Index. There were 11 turtles that have been caught. The results indicated the growth of straight carapace length notch to tip affect the growth of the other body parts of hawksbill turtle and based approach to geometry, shape growth of hawksbill turtles that are found in Maratua not changed but only have added size. Based on the health status through observation epibionts, mechanical damages and analysis of Body Condition Index, hawksbill in Maratua still relatively healthy compared with studies elsewhere. Keywords: Eretmochelys imbricata (Linnaeus, 1766); geometry; Maratua; morphometr

Simulation par éléments finis de la déformation de polycristaux à partir d'images de tomographie par contraste de diffraction

National audienceLa tomographie par contraste de diffraction donne accès à la forme, l'orientation et l'état de déformation élastique des grains dans des volumes polycristallins pouvant contenir à l'heure actuelle jusqu'à mille grains. La combinaison de cette technique avec la méthode des éléments finis est particuliè- rement prometteuse pour analyser le rôle de la cristallographie locale sur les mécanismes de déformation et de dégradation dans des matériaux polycristallins. Dans ce travail, un échantillon polycristallin de titane est imagé en 3D puis maillé et sa déformation de traction est calculée par éléments finis.See http://hal.archives-ouvertes.fr/docs/00/59/29/18/ANNEX/r_974AO844.pd

The grain microstructure of polycrystalline materials as revealed by the combined use of synchrotron X-ray imaging and diffraction techniques

National audienceCombining the principles of x-ray imaging and diffraction techniques, it has recently become possible to map the 3D grain microstructure in a range of polycrystalline materials. Associating this 3D orientation mapping with conventional attenuation and/or phase contrast tomography yields a non-destructive characterization technique, enabling time-lapse observation of dynamic processes in the bulk of structural materials. The capabilities and limitations., as well as future perspectives of this new characterization approach will be discussed and illustrated on selected application examples

Characterization of polycrystalline materials by X-ray diffraction contrast tomography

National audienceSynchrotron based X-ray imaging and diffraction techniques offer interesting possibilities for characterizing the grain microstructure in a variety of polycrystalline mono-and multiphase materials. Direct visualization of the three-dimensional grain boundary network or of two-phase (duplex) grain structures by means of absorption and/or phase contrast techniques is possible, but restricted to specific material systems (Ludwig 2009b). However, conventional attenuation or phase contrast imaging techniques do not give access to the crystallographic orientation of the grains and additional diffraction measurements are required. On the other hand, three-dimensional X-ray diffraction methods like Differential Aperture X-ray Microscopy (DAXM, (Larson, 2002) or 3D X-ray Diffraction Microscopy (3DXRD) (Poulsen, 2004) can analyse the 3D grain structure and/or elastic strain tensors of individual grains in polycrystalline materials, but are blind to the microstructural features (inclusions, cracks and porosity) visible in attenuation and/or phase contrast imaging techniques . A recent extension of the 3DXRD methodology, termed X-ray diffraction contrast tomography (DCT) (Ludwig 2009a), combines the principles of 3DXRD and X-ray absorption tomography. With a single scan, DCT can provide simultaneous access to the grain shape, crystallographic orientation, full elastic strain tensor and the local attenuation coefficient distribution in three dimensions. The technique applies to a range of plastically undeformed, polycrystalline mono-phase materials, fulfilling some conditions on grain size and texture. The straightforward combination with in-situ microtomographic observations opens interesting new possibilities for the characterization of microstructure related damage and deformation mechanisms in these materials

Correlative Nanoscale 3D Imaging of Structure and Composition in Extended Objects

Structure and composition at the nanoscale determine the behavior of biological systems and engineered materials. The drive to understand and control this behavior has placed strong demands on developing methods for high resolution imaging. In general, the improvement of three-dimensional (3D) resolution is accomplished by tightening constraints: reduced manageable specimen sizes, decreasing analyzable volumes, degrading contrasts, and increasing sample preparation efforts. Aiming to overcome these limitations, we present a non-destructive and multiple-contrast imaging technique, using principles of X-ray laminography, thus generalizing tomography towards laterally extended objects. We retain advantages that are usually restricted to 2D microscopic imaging, such as scanning of large areas and subsequent zooming-in towards a region of interest at the highest possible resolution. Our technique permits correlating the 3D structure and the elemental distribution yielding a high sensitivity to variations of the electron density via coherent imaging and to local trace element quantification through X-ray fluorescence. We demonstrate the method by imaging a lithographic nanostructure and an aluminum alloy. Analyzing a biological system, we visualize in lung tissue the subcellular response to toxic stress after exposure to nanotubes. We show that most of the nanotubes are trapped inside alveolar macrophages, while a small portion of the nanotubes has crossed the barrier to the cellular space of the alveolar wall. In general, our method is non-destructive and can be combined with different sample environmental or loading conditions. We therefore anticipate that correlative X-ray nano-laminography will enable a variety of in situ and in operando 3D studies

Thoughts about the optimum data acquisition geometry and time resolution of monochromatic beam x-ray diffraction microscopy experiments

International audienceSo far, 3D X-ray diffraction microscopy (3DXRD) and X-ray diffraction contrast tomography (DCT) experiments have typically been performed in forward scattering geometry, the detector intercepting diffraction cones up to limited opening angles below 30°. The extension of the current synchrotron–based methodology towards 3D orientation mapping of deformed microstructures with down to (sub-)micrometer spatial resolution will require a reduction of the pixel and hence sample size by one order of magnitude. This in turn relaxes the need for high energy beams and opens interesting possibilities for new acquisition geometries, taking advantage of improved spatial resolution and strain sensitivity at high diffraction angles. First results obtained in this acquisition geometry will be discussed and a comparison to polychromatic micro-diffraction experiments is drawn

[La microstructure 3D des matériaux polycristallins vue sous la lumière synchrotron]

International audienceSynchrotron radiation X-ray imaging and diffraction techniques offer new possibilities for non-destructive bulk characterization of polycrystalline materials. Minute changes in electron density (different crystallographic phases, cracks, porosities) can be detected using 3D imaging modes exploiting Fresnel diffraction and the coherence properties of third generation synchrotron beams. X-ray diffraction contrast tomography, a technique based on Bragg diffraction imaging, provides access to the 3D shape, orientation and elastic strain state of the individual grains from polycrystalline sample volumes containing several hundred up to a few thousand grains. Combining both imaging modalities allows a comprehensive description of the microstructure of the material at the micrometer length scale. Repeated observations during (interrupted) mechanical tests provide unprecedented insight into crystallographic and grain microstructure related aspects of polycrystal deformation and degradation mechanisms in materials, fulfilling some conditions on grain size and deformation state.Les techniques d'imagerie et de diffraction au rayonnement synchrotron offrent de nouvelles possibilités pour la caractérisation tridimensionnelle et non destructive des matériaux polycristallins. De faibles variations de densité électronique (phases secondaires, fissures, porosités) peuvent êtres détectées grâce à des modes d'imagerie qui exploitent la diffraction de Fresnel ainsi que la cohérence des faisceaux issus des sources synchrotron de troisième génération. La tomographie par contraste de diffraction, autre technique d'imagerie 3D basée sur la diffraction de Bragg, donne accès à la forme, l'orientation et l'état de déformation élastique des grains dans des volumes polycristallins contenant jusqu'à mille grains. La combinaison de ces deux modes d'imagerie permet de caractériser des matériaux polycristallins à l'échelle du micron. Des observations répétées lors d'essais mécaniques (interrompus) permettent d'analyser le rôle de la cristallographie locale sur les mécanismes de déformation et de dégradation dans des matériaux polycristallins, respectant certaines conditions sur la taille de grains, et/ou leur état de déformation

Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae

Lesion and transplantation studies in the cockroach, Leucophaea maderae, have located its bilaterally symmetric circadian pacemakers necessary for driving circadian locomotor activity rhythms to the accessory medulla of the optic lobes. The accessory medulla comprises a network of peptidergic neurons, including pigment-dispersing factor (PDF)-expressing presumptive circadian pacemaker cells. At least three of the PDF-expressing neurons directly connect the two accessory medullae, apparently as a circadian coupling pathway. Here, the PDF-expressing circadian coupling pathways were examined for peptide colocalization by tracer experiments and double-label immunohistochemistry with antisera against PDF, FMRFamide, and Asn13-orcokinin. A fourth group of contralaterally projecting medulla neurons was identified, additional to the three known groups. Group one of the contralaterally projecting medulla neurons contained up to four PDF-expressing cells. Of these, three medium-sized PDF-immunoreactive neurons coexpressed FMRFamide and Asn13-orcokinin immunoreactivity. However, the contralaterally projecting largest PDF neuron showed no further peptide colocalization, as was also the case for the other large PDF-expressing medulla cells, allowing the easy identification of this cell group. Although two-thirds of all PDF-expressing medulla neurons coexpressed FMRFamide and orcokinin immunoreactivity in their somata, colocalization of PDF and FMRFamide immunoreactivity was observed in only a few termination sites. Colocalization of PDF and orcokinin immunoreactivity was never observed in any of the terminals or optic commissures. We suggest that circadian pacemaker cells employ axonal peptide sorting to phase-control physiological processes at specific times of the day

Three-dimensional grain mapping by x-ray diffraction contrast tomography and the use of Friedel pairs in diffraction data analysis

X-ray diffraction contrast tomography (DCT) is a technique for mapping grain shape and orientation in plastically undeformed polycrystals. In this paper, we describe a modified DCT data acquisition strategy which permits the incorporation of an innovative Friedel pair method for analyzing diffraction data. Diffraction spots are acquired during a 360 degree rotation of the sample and are analyzed in terms of the Friedel pairs ((hkl) and (hkl -) reflections, observed 180 degrees apart in rotation). The resulting increase in the accuracy with which the diffraction vectors are determined allows the use of improved algorithms for grain indexing (assigning diffraction spots to the grains from which they arise) and reconstruction. The accuracy of the resulting grain maps is quantified with reference to synchrotron microtomography data for a specimen made from a beta titanium system in which a second phase can be precipitated at grain boundaries, thereby revealing the grain shapes. The simple changes introduced to the DCT methodology are equally applicable to other variants of grain mapping. Copyright 2009 American Institute of Physics