Sea-Ice Production in Antarctic Coastal Polynyas Estimated From AMSR2 Data and Its Validation Using AMSR-E and SSM/I-SSMIS Data

Antarctic coastal polynyas are very high sea-ice production areas. The resultant large amount of brine rejection leads to the formation of dense water. The dense water forms Antarctic bottom water, which is the densest water in the global overturning circulation and a key player in climate change as a significant sink for heat and carbon dioxide. In this study, an algorithm was developed that uses Advanced Microwave Scanning Radiometer 2 (AMSR2) data (2012-present) to detect polynya area and estimates thin ice thickness by a method similar to that used to develop the algorithm for Advanced Microwave Scanning Radiometer for EOS (AMSR-E) data. Landfast sea-ice areas were also detected using AMSR2 data. Ice production in the polynyas was estimated by a heat flux calculation using AMSR2 sea-ice data. In four major polynyas, AMSR2 ice production was compared with AMSR-E (2003-2011) ice production through comparison of them with Special Sensor Microwave Imager (SSM/I) and Special Sensor Microwave Imager/Sounder (SSMIS) ice production. The comparison confirmed that the ice production from AMSR-E/2 data, which have higher spatial resolution than SSM/I-SSMIS data, can be used to analyze time series covering more than 10 years. For example, maps of annual ice production based on AMSR-E/2 data revealed detailed changes of the Mertz Polynya, where the ice production decreased significantly after the Mertz Glacier Tongue calving in 2010. Continuous monitoring of the coastal polynyas by the AMSR series sensors is essential for climate-change-related analyses in the Antarctic Ocean

The Outsider and Sunk Cost Effects on 'Dango' in Public Procurement Bidding: An Experimental Analysis.

This paper presents the result of experiments for finding some insight into an effect of the new bidding system in Japanese public construction works procurement on bidders' collusion, which is called 'dango'. We focused on an effect of the entry of an outsider who is not a dango member. The main conclusion of the experiments is that an outsider, a subject who is not allowed to communicate with other subjects, has a robust effect to prevent other subjects from colluding and to make the winning price decrease considerably.CONSTRUCTION ; PRICES ; BIDDING

Transcription of Endogenous Retrovirus Group K Members and Their Neighboring Genes in Chicken Skeletal Muscle Myoblasts

Skeletal muscle myoblasts are myogenic precursor cells that generate myofibers during muscle development and growth. We recently reported that broiler myoblasts, compared to layer myoblasts, proliferate and differentiate more actively and promptly into myocytes, which corresponds well with the muscle phenotype of broilers. Furthermore, RNA sequencing (RNA-seq) revealed that numerous genes are differentially expressed between layer and broiler myoblasts during myogenic differentiation. Based on the RNA-seq data, we herein report that chicken myoblasts transcribe endogenous retrovirus group K member (ERVK) genes. In total, 16 ERVKs were highly expressed in layer myoblasts and two (termed BrK1 and BrK2) were significantly induced in broiler myoblasts. These transcribed ERVKs had a totalof 182 neighboring genes within ±100 kb on the chromosomes, of which 40% were concentrated within ±10 kb of the ERVKs. We further investigated whether the transcription of ERVKs affects the expression of their neighboring genes. BrK1 had two neighboring genes; LOC107052719 was overlapping with BrK1 and downregulated in the broiler myoblasts, and FAM19A2 was upregulated in the broiler myoblasts as well as BrK1. BrK2 had 14 neighboring genes, and only one gene, LOC772243, was differentially expressed between layer and broiler myoblasts. LOC772243 was overlapping with BrK2 and suppressed in the broiler myoblasts. These data indicate that the transcription of ERVKs may impact the expression of their neighboring genes in chicken myoblasts.ArticleThe Journal of Poultry Science. 58(2) :79-87(2021)journal articl

Toll -like receptor ligand-dependent inflammatory responses in chick skeletal muscle myoblasts

Epub 2018 Oct 30Toll-like receptors (TLRs) are a group of sensory receptors which are capable of recognizing a microbial invasion and activating innate immune system responses, including inflammatory responses, in both immune and non immune cells. However, TLR functions in chick myoblasts, which are myogenic precursor cells contributing to skeletal muscle development and growth, have not been studied. Here, we report the expression patterns of TLR genes as well as TLR ligand-dependent transcriptions of interleukin (IL) genes in primary-cultured chick myoblasts. Almost TLR genes were expressed both in layer and broiler myoblasts but TLR1A was detected only in embryonic layer chick myoblasts. Chick TLR1/2 ligands, Pam(3)CSK(4) and FSL-1, induced inflammatory ILs in both layer and broiler myoblasts but a TLR4 ligand, lipopolysaccharide, scarcely promoted. This is the first report on TLR ligand-dependent inflammatory responses in chick myoblasts, which may provide useful information to chicken breeding and meat production industries.ArticleDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY. 91:115-122 (2019)journal articl

Berberine and palmatine inhibit the growth of human rhabdomyosarcoma cells

Published online: 29 Aug 2019A natural isoquinoline alkaloid, berberine, has been known to exhibit anti-tumor activity in various cancer cells via inducing cell cycle arrest. However, it has not been investigated whether berberine and its analogs inhibit the growth of rhabdomyosarcoma (RMS), which is the most frequent soft tissue tumor in children. The present study examined the anti-tumor effects of berberine and palmatine on expansions of three human embryonal RMS cell lines; ERMS1, KYM1, and RD. Intracellular incorporation of berberine was relatively higher than that of palmatine in every RMS cell line. Berberine significantly inhibited the cell cycle of all RMS cells at G(1) phase. On the other hand, palmatine only suppressed the growth of RD cells. Both of berberine and palmatine strongly inhibited the growth of tumorsphere of RD cells in three-dimensional culture. These results indicate that berberine derivatives have the potential of anti-tumor drugs for RMS therapy.ArticleBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 84(1):63-75 (2020)journal articl

Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts

Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products.ArticleANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017)journal articl

Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin

Herein we report that the 18-base telomeric oligodeoxynucleotides (ODNs) designed from the Lactobacillus rhamnosus GG genome promote differentiation of skeletal muscle myoblasts which are myogenic precursor cells. We termed these myogenetic ODNs (myoDNs). The activity of one of the myoDNs, iSN04, was independent of Toll-like receptors, but dependent on its conformational state. Molecular simulation and iSN04 mutants revealed stacking of the 13–15th guanines as a core structure for iSN04. The alkaloid berberine bound to the guanine stack and enhanced iSN04 activity, probably by stabilizing and optimizing iSN04 conformation. We further identified nucleolin as an iSN04-binding protein. Results showed that iSN04 antagonizes nucleolin, increases the levels of p53 protein translationally suppressed by nucleolin, and eventually induces myotube formation by modulating the expression of genes involved in myogenic differentiation and cell cycle arrest. This study shows that bacterial-derived myoDNs serve as aptamers and are potential nucleic acid drugs directly targeting myoblasts.ArticleFRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 8:616706 (2021)journal articl