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    Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

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    Additional file 3. Evaluation of reference gene stability. The non-normalized raw Cq-values of reference small ncRNAs for adult (n=12 Tempus tubes) and cord blood (n=3 Tempus tubes) samples collected in the Tempus tubes of the blood-RNA samples isolated using the three RNA isolation protocols

    MOESM1 of Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

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    Additional file 1. Target abundance of RNA transcripts. Evaluation of target abundance; i.e., whether low abundant RNA transcripts (RNA transcripts with Cq-values above 30 cycles) could be detected in blood-RNA samples isolated using the five blood-RNA isolation protocols. Low abundant mRNA and miRNA transcripts were detected in all analyzed samples. The average of non-normalized raw Cq-values of all targets from all adult (n=11-12 Tempus tubes) and cord blood (n=3-6 Tempus tubes) samples collected in the Tempus tubes of the blood-RNA samples isolated using the five RNA isolation protocols
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