784 research outputs found

    DataSheet_1_Transcriptomic and metabolomic profiling reveals the drought tolerance mechanism of Illicium difengpi (Schisandraceae).zip

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    Illicium difengpi (Schisandraceae), an endangered medicinal plant endemic to karst areas, is highly tolerant to drought and thus can be used as an ideal material for investigating adaptive mechanism to drought stress. The understanding of the drought tolerance of I. difengpi, especially at the molecular level, is lacking. In the present study, we aimed to clarify the molecular mechanism underlying drought tolerance in endemic I. difengpi plant in karst regions. The response characteristics of transcripts and changes in metabolite abundance of I. difengpi subjected to drought and rehydration were analyzed, the genes and key metabolites responsive to drought and rehydration were screened, and some important biosynthetic and secondary metabolic pathways were identified. A total of 231,784 genes and 632 metabolites were obtained from transcriptome and metabolome analyses, and most of the physiological metabolism in drought-treated I. difengpi plants recovered after rehydration. There were more upregulated genes than downregulated genes under drought and rehydration treatments, and rehydration treatment induced stable expression of 65.25% of genes, indicating that rehydration alleviated drought stress to some extent. Drought and rehydration treatment generated flavonoids, phenolic acids, flavonols, amino acids and their derivatives, as well as metabolites such as saccharides and alcohols in the leaves of I. difengpi plants, which alleviated the injury caused by excessive reactive oxygen species. The integration of transcriptome and metabolome analyses showed that, under drought stress, I. difengpi increased glutathione, flavonoids, polyamines, soluble sugars and amino acids, contributing to cell osmotic potential and antioxidant activity. The results show that the high drought tolerance and recovery after rehydration are the reasons for the normal growth of I. difengpi in karst mountain areas.</p

    DataSheet_2_Transcriptomic and metabolomic profiling reveals the drought tolerance mechanism of Illicium difengpi (Schisandraceae).zip

    No full text
    Illicium difengpi (Schisandraceae), an endangered medicinal plant endemic to karst areas, is highly tolerant to drought and thus can be used as an ideal material for investigating adaptive mechanism to drought stress. The understanding of the drought tolerance of I. difengpi, especially at the molecular level, is lacking. In the present study, we aimed to clarify the molecular mechanism underlying drought tolerance in endemic I. difengpi plant in karst regions. The response characteristics of transcripts and changes in metabolite abundance of I. difengpi subjected to drought and rehydration were analyzed, the genes and key metabolites responsive to drought and rehydration were screened, and some important biosynthetic and secondary metabolic pathways were identified. A total of 231,784 genes and 632 metabolites were obtained from transcriptome and metabolome analyses, and most of the physiological metabolism in drought-treated I. difengpi plants recovered after rehydration. There were more upregulated genes than downregulated genes under drought and rehydration treatments, and rehydration treatment induced stable expression of 65.25% of genes, indicating that rehydration alleviated drought stress to some extent. Drought and rehydration treatment generated flavonoids, phenolic acids, flavonols, amino acids and their derivatives, as well as metabolites such as saccharides and alcohols in the leaves of I. difengpi plants, which alleviated the injury caused by excessive reactive oxygen species. The integration of transcriptome and metabolome analyses showed that, under drought stress, I. difengpi increased glutathione, flavonoids, polyamines, soluble sugars and amino acids, contributing to cell osmotic potential and antioxidant activity. The results show that the high drought tolerance and recovery after rehydration are the reasons for the normal growth of I. difengpi in karst mountain areas.</p

    DataSheet1_Transcriptome-wide analysis of trigeminal ganglion and subnucleus caudalis in a mouse model of chronic constriction injury-induced trigeminal neuralgia.zip

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    Trigeminal neuropathic pain (TNP) induces mechanical allodynia and hyperalgesia, which are known to alter gene expression in injured dorsal root ganglia primary sensory neurons. Non-coding RNAs (ncRNAs) have been linked to TNP. However, the functional mechanism underlying TNP and the expression profile of ncRNAs in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Sp5C) are still unknown. We used RNA sequencing and bioinformatics analysis to examine the TG and Sp5C transcriptomes after infraorbital nerve chronic constrictive injury (IoN-CCI). The robust changes in the gene expression of lncRNAs, circRNAs, and mRNAs were observed within the TG and Sp5C from mice that underwent IoN-CCI and the sham-operated mice (day 7). In total, 111,003 lncRNAs were found in TG and 107,157 in Sp5C; 369 lncRNAs were differentially expressed in TG, and 279 lncRNAs were differentially expressed in Sp5C. In addition, 13,216 circRNAs in TG and 21,658 circRNAs in Sp5C were identified, with 1,155 circRNAs and 2,097 circRNAs differentially expressed in TG and Sp5C, respectively. Furthermore, 5,205 DE mRNAs in TG and 3,934 DE mRNAs in Sp5C were differentially expressed between IoN-CCI and sham groups. The study revealed a high correlation of pain-related differentially expressed genes in the TG and Sp5C to anxiety, depression, inflammation, neuroinflammation, and apoptosis. Gene Ontology analysis revealed that binding-related molecular functions and membrane-related cell components were significantly enriched. Kyoto Encyclopedia of Genes and Genomes analysis shows the most significant enrichments in neurogenesis, nervous system development, neuron differentiation, adrenergic signaling, cAMP signaling, MAPK signaling, and PI3K-Akt signaling pathways. Furthermore, protein–protein interaction analysis showed that hub genes were implicated in neuropeptide signaling pathways. Functional analysis of DE ncRNA-targeting genes was mostly enriched with nociception-related signaling pathways underpinning TNP. Our findings suggest that ncRNAs are involved in TNP development and open new avenues for research and treatment.</p

    Additional file 1 of ADP-dependent glucokinase controls metabolic fitness in prostate cancer progression

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    Additional file 1. Table S1 Primary antibodies used in this study. Table S2 siRNA sequences. Table S3 sgRNAs sequences. Table S4 Primers used in this study. Table S5 Univariate Cox regression analysis. Fig. S1 Function and expression of genes involved in the oxidative phosphorylation of glucose and their associations with clinical outcomes. Fig. S2 Cell type enrichment and prognosis of PRAD subgroups based on ADPGK expression. Fig. S3 Association between ADPGK and PCa immune status. Fig. S4 qPCR results showed the stable overexpression of ADPGK in PCa cells after lentivirus transfection. Fig. S5 The impact of ADPGK knockdown on LNCaP cell viability was assessed by CCK-8 assay. Fig. S6 Hematoxylin and eosin (HE) staining of lung tissues of mice bearing PC3 cells

    DataSheet1_Study on the seismic response of new staggered story isolated structure under different parameters.xls

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    The new staggered story isolated structure is developed according to the base-isolated structure and the mid-story isolated structure. Quantitative calculation and evaluation of seismic damage are very important for structural safety. In this paper, the seismic damage evaluation of a new staggered story isolated structure is studied by numerical simulation and damage index calculation. A new staggered story isolated structure is established, and the effects of different layers and different chassis areas on the seismic response of the structure are studied. When the position of the bottom isolated layer stays the same, the upper isolated layer is set at different layers, which is set to the top of the 3rd, 6th and 9th layers. When the upper isolated layer keeps at the top of the 3rd layer, the chassis area is set at a different area, which is 26 m × 26 m, 36 m × 36 m and 46 m × 46 m. The results show that the new staggered story isolated structure has good isolated effects under the ground motion. For the structure set upper isolation layer is lower, the inter-layer shear force, inter-layer acceleration and inter-layer displacement are reduced. The energy dissipation effect of the structure improves. The core tube is less damaged and the plastic hinge is smaller. With the increase of chassis area, the isolated effect of the part above the upper isolated layer is good, while the shear force and acceleration of the part below the upper isolated layer of the structure increase, the damage at the core tube changed little and the appearance of the plastic hinge increased. Under earthquakes, with the change in position of the upper isolated layer and the area of the chassis of the new staggered story isolated structure, the displacement, tensile stress and compressive stress of the isolated bearing still meet the requirements of the standard.</p

    Data_Sheet_1_Untreated depression and anxiety in patients with common skin diseases: a cross-sectional study in China.docx

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    ObjectiveThe study aimed to analyze the current status and reasons for the neglect of the psychological need of patients with common skin diseases.MethodsThis cross-sectional study was conducted in China using an online self-assessment questionnaire distributed via social media. Demographic, clinical and psychological data were collected, and the main outcomes, i.e., depression (evaluated using the 9-item Patient Health Questionnaire, PHQ-9) and anxiety (evaluated using Generalized Anxiety Disorder-7, GAD-7). Multivariate regression analysis was used for the prediction of variates of mental health service seeking behaviors.ResultsA total of 1,010 patients participated in the survey, and 273 (27.0%) patients met the “with need” criteria, i.e., having the need for mental health intervention but not being treated. In the multivariate regression model, income (OR = 0.80, 95%CI: 0.65–0.99), duration of disease (OR = 0.68, 95%CI: 0.49–0.95) and suicide ideation (OR = 2.10, 95%CI: 1.14–3.87) were significant factors. For patients who did not receive mental health care, the lack of knowledge about the availability of mental health services, lack of knowledge of where to seek help, concerns about the side effects of treatment, failure to seek treatment for severe skin diseases, and absence of current psychological distress were associated with their need for psychological intervention.ConclusionThis study examined the current status of the need for psychological intervention and the reasons why the need was unmet in patients with skin diseases. Due to the confusion and a lack of knowledge about their mental health issues, mental health services are often underutilized.</p

    Quantum Chemistry Insight into the Multifaceted Structural Properties of Two-Dimensional Covalent Organic Frameworks

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    2D covalent organic frameworks (COFs) possess a wide range of desirable mechanical and optoelectronic properties, rendering them a versatile compound class that is widely investigated in many areas of chemistry. However, experimental structure determination remains challenging as mostly inseparable isomers are measured while computational models mostly account for a very limited number of model structures, making the establishment of comprehensive structure–property relationships difficult. Thus, here, we go computationally beyond the conventional but insufficient description of eclipsed stacked COF building blocks. We emphasize the presence of a variety of stacking and structural motifs and investigate their impact on the electronic properties. Given the very large size of the investigated systems, we employ the fast and accurate tight-binding based semiempirical quantum mechanical (SQM) GFN-xTB method. Taking an imine-linked COF as a representative system, we discuss our structural, energetic, electronic, and spectroscopic results calculated at the periodic GFN1-xTB quantum chemistry level in comparison to experimental findings
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