1,351 research outputs found
Lipid Ion Channels
The interpretation electrical phenomena in biomembranes is usually based on
the assumption that the experimentally found discrete ion conduction events are
due to a particular class of proteins called ion channels while the lipid
membrane is considered being an inert electrical insulator. The particular
protein structure is thought to be related to ion specificity, specific
recognition of drugs by receptors and to macroscopic phenomena as nerve pulse
propagation. However, lipid membranes in their chain melting regime are known
to be highly permeable to ions, water and small molecules, and are therefore
not always inert. In voltage-clamp experiments one finds quantized conduction
events through protein-free membranes in their melting regime similar to or
even undistinguishable from those attributed to proteins. This constitutes a
conceptual problem for the interpretation of electrophysiological data obtained
from biological membrane preparations. Here, we review the experimental
evidence for lipid ion channels, their properties and the physical chemistry
underlying their creation. We introduce into the thermodynamic theory of
membrane fluctuations from which the lipid channels originate. Furthermore, we
demonstrate how the appearance of lipid channels can be influenced by the
alteration of the thermodynamic variables (temperature, pressure, tension,
chemical potentials) in a coherent description that is free of parameters. This
description leads to pores that display dwell times closely coupled to the
fluctuation lifetime via the fluctuation-dissipation theorem. Drugs as
anesthetics and neurotransmitters are shown to influence the channel likelihood
and their lifetimes in a predictable manner. We also discuss the role of
proteins in influencing the likelihood of lipid channel formation.Comment: Revie
Linear nonequilibrium thermodynamics of reversible periodic processes and chemical oscillations
Onsager's phenomenological equations successfully describe irreversible
thermodynamic processes. They assume a symmetric coupling matrix between
thermodynamic fluxes and forces. It is easily shown that the antisymmetric part
of a coupling matrix does not contribute to dissipation. Therefore, entropy
production is exclusively governed by the symmetric matrix even in the presence
of antisymmetric terms. In this work we focus on the antisymmetric
contributions which describe isentropic oscillations and well-defined equations
of motion. The formalism contains variables that are equivalent to momenta, and
coefficients that are analogous to an inertial mass. We apply this formalism to
simple problems such as an oscillating piston and the oscillation in an
electrical LC-circuit. We show that isentropic oscillations are possible even
close to equilibrium in the linear limit and one does not require far-from
equilibrium situations. One can extend this formalism to other pairs of
variables, including chemical systems with oscillations. In isentropic
thermodynamic systems all extensive and intensive variables including
temperature can display oscillations reminiscent of adiabatic waves.Comment: 11 pages, 5 figure
Periodic solutions and refractory periods in the soliton theory for nerves and the locust femoral nerve
Close to melting transitions it is possible to propagate solitary
electromechanical pulses which reflect many of the experimental features of the
nerve pulse including mechanical dislocations and reversible heat production.
Here we show that one also obtains the possibility of periodic pulse generation
when the boundary condition for the nerve is the conservation of the overall
length of the nerve. This condition generates an undershoot beneath the
baseline (`hyperpolarization') and a `refractory period', i.e., a minimum
distance between pulses. In this paper, we outline the theory for periodic
solutions to the wave equation and compare these results to action potentials
from the femoral nerve of the locust (locusta migratoria). In particular, we
describe the frequently occurring minimum-distance doublet pulses seen in these
neurons and compare them to the periodic pulse solutions.Comment: 10 pages, 6 Figure
The capacitance and electromechanical coupling of lipid membranes close to transitions. The effect of electrostriction
Biomembranes are thin capacitors with the unique feature of displaying phase
transitions in a physiologically relevant regime. We investigate the voltage
and lateral pressure dependence of their capacitance close to their chain
melting transition. Since the gel and the fluid membrane have different area
and thickness, the capacitance of the two membrane phases is different. In the
presence of external fields, charges exert forces that can influence the state
of the membrane, thereby influencing the transition temperature. This
phenomenon is called electrostriction. We show that this effect allows us to
introduce a capacitive susceptibility that assumes a maximum in the melting
transition with an associated excess charge. As a consequence, there exist
voltage regimes where a small change in voltage can lead to a large uptake of
charge and a large capacitive current. Furthermore, we consider
electromechanical behavior such as pressure-induced changes in capacitance, and
the application of such concepts in biology.Comment: 5 figure
Lipid ion channels and the role of proteins
Synthetic lipid membranes in the absence of proteins can display quantized
conduction events for ions that are virtually indistinguishable from those of
protein channel. By indistinguishable we mean that one cannot decide based on
the current trace alone whether conductance events originate from a membrane,
which does or does not contain channel proteins. Additional evidence is
required to distinguish between the two cases, and it is not always certain
that such evidence can be provided. The phenomenological similarities are
striking and span a wide range of phenomena: The typical conductances are of
equal order and both lifetime distributions and current histograms are similar.
One finds conduction bursts, flickering, and multistep-conductance. Lipid
channels can be gated by voltage, and can be blocked by drugs. They respond to
changes in lateral membrane tension and temperature. Thus, they behave like
voltage-gated, temperature-gated and mechano-sensitive protein channels, or
like receptors. Lipid channels are remarkably under-appreciated. However, the
similarity between lipid and protein channels poses an eminent problem for the
interpretation of protein channel data. For instance, the Hodgkin-Huxley theory
for nerve pulse conduction requires a selective mechanism for the conduction of
sodium and potassium ions. To this end, the lipid membrane must act both as a
capacitor and as an insulator. Non-selective ion conductance by mechanisms
other than the gated protein-channels challenges the proposed mechanism for
pulse propagation. ... Some important questions arise: Are lipid and protein
channels similar due a common mechanism, or are these similarities fortuitous?
Is it possible that both phenomena are different aspects of the same
phenomenon? Are lipid and protein channels different at all? ... (abbreviated)Comment: 10 pages, 10 figures - accepted by 'Accounts of Chemical Research
The thermodynamics of general and local anesthesia
General anesthetics are known to cause depression of the freezing point of
transitions in biomembranes. This is a consequence of ideal mixing of the
anesthetic drugs in the membrane fluid phase and exclusion from the solid
phase. Such a generic law provides physical justification of the famous
Meyer-Overton rule. We show here that general anesthetics, barbiturates and
local anesthetics all display the same effect on melting transitions. Their
effect is reversed by hydrostatic pressure. Thus, the thermodynamic behavior of
local anesthetics is very similar to that of general anesthetics. We present a
detailed thermodynamic analysis of heat capacity profiles of membranes in the
presence of anesthetics. This analysis is able to describe experimentally
observed calorimetric profiles and permits prediction of the anesthetic
features of arbitrary molecules. In addition, we discuss the thermodynamic
origin of the cutoff-effect of long-chain alcohols and the additivity of the
effect of general and local anesthetics.Comment: 12 pages, 9 figures, 1 tabl
The stability of solitons in biomembranes and nerves
We examine the stability of a class of solitons, obtained from a
generalization of the Boussinesq equation, which have been proposed to be
relevant for pulse propagation in biomembranes and nerves. These solitons are
found to be stable with respect to small amplitude fluctuations. They emerge
naturally from non-solitonic initial excitations and are robust in the presence
of dissipation.Comment: 7 pages, 5 figure
Ion-channel-like behavior in lipid bilayer membranes at the melting transition
It is well known that at the gel-liquid phase transition temperature a lipid
bilayer membrane exhibits an increased ion permeability. We analyze the
quantized currents in which the increased permeability presents itself. The
open time histogram shows a "-3/2" power law which implies an open-closed
transition rate that decreases like as time evolves. We
propose a "pore freezing" model to explain the observations. We discuss how
this model also leads to the noise that is commonly observed in
currents across biological and artificial membranes.Comment: 5 pages, 4 figure
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