Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-2

T manner (5% and 10% FBS), and increased migration was significantly blocked by 40 μg/ml heparin. **, < 0.01, compared with the same treatment of U251n-pc cells. $, < 0.01, compared with 5% FBS group of U251n-hpa. #, P < 0.01, compared with 10% FBS group of U251n-hpa. (B) SDF-1 significantly increased cell chomatactic migration in the presence of 10% FBS medium. Increased migration was not observed when SDF-1 was added in serum free medium. **, < 0.01, compared with the same treatment of U251n-pc cells. #, < 0.01, compared with 10%FBS group of each cell line.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-4

-GSK3. β1 integrin, phospho-FAK and phospho-ERK levels were not significantly changed.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-0

By Western blot. Both U251n and U87 exhibited baseline level of heparanase expression. Latent form of heparanase was detected in HF2303 cells. Increased heparanase expression, both the latent (65 kDa) and active (50 kDa) forms, was observed in cell lysate derived from U251n-hpa cells, as compared to U251n-pc control cells. (B) U251n-hpa cells expressed about 100 times more mRNA than U251n-pc cells. (C) Increased heparanase activity was detected in U251n-hpa cells.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-1

D by BD invasion assay. Increased cell invasion was greatly inhibited by 40 μg/ml heparin. **, < 0.01, compared with U251n-pc cells. #, < 0.01, compared with U251n-hpa group of no heparin treatment. (B) mRNA levels of MMP-2 and MMP-9 showed no significant difference between U251n-pc and U251n-hpa cells. (C) No significant differences were detected in MMP-2 and MMP-9 activity between U251n-hpa cells and U251n-pc cells as measured by zymography assay.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

Letters adjacent to boxed areas in schematic representation of a brain coronal section (A) indicate areas where representative confocal microscopic images were taken.

<p>Panels B and C show YFP (green) and DCX (red) positive cells in non-ischemic (B) and ischemic SVZ (C) in middle-aged mice 30 days after stroke. Orthogonal views (D, E) show that a YFP immunoreactive cell (green) was DCX positive (D, red) or NeuN (NN) positive (E) in the ischemic striatum. Quantitative data analysis (F) shows percentage of YFP/DCX and YFP/NeuN positive cells in the ischemic striatum after treatment with saline and Sildenafil. *p<0.05 vs the saline group. n = 10/saline and n = 11/Sildenafil. Bar = 10 µm for B to E. Blue color  =  cell nuclei. CC  =  corpus callosum, and LV  =  lateral ventricle.</p

Tadalafil increases axonal remodeling in diabetic db/db mice.

<p>Panels A to C show semi-thin toluidine blue-stained cross sections of sciatic nerves from a representative non-diabetic mouse (DM, A), diabetic mouse treated with saline (DB, B), and diabetic mouse treated with tadalafil (DBTA, C). The table shows the effect of tadalafil on histomorphometric parameter of sciatic nerves. Values are mean±SE. *p<0.05 and #p<0.05 versus the non-diabetic mouse (DM) and the diabetic mouse treated with saline (DB), respectively. n = 10/group. Bar = 20μm. DBTA = diabetic mouse treated with tadalafil.</p

Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-6

D by BD invasion assay. Increased cell invasion was greatly inhibited by 40 μg/ml heparin. **, < 0.01, compared with U251n-pc cells. #, < 0.01, compared with U251n-hpa group of no heparin treatment. (B) mRNA levels of MMP-2 and MMP-9 showed no significant difference between U251n-pc and U251n-hpa cells. (C) No significant differences were detected in MMP-2 and MMP-9 activity between U251n-hpa cells and U251n-pc cells as measured by zymography assay.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells-3

Seeding, the numbers of U251n-hpa cells were significantly increased as compared to U251n-pc cells. **, < 0.01, compared with U251n-pc cells. (B) U251n-hpa cells developed more and bigger colonies than U251n-pc cells as measured by colony formation assay. **, < 0.01, compared with U251n-pc cells.<p><b>Copyright information:</b></p><p>Taken from "Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells"</p><p>http://www.jeccr.com/content/27/1/23</p><p>Journal of Experimental & Clinical Cancer Research : CR 2008;27(1):23-23.</p><p>Published online 22 Jul 2008</p><p>PMCID:PMC2499998.</p><p></p

A diagram shows experimental protocols (A).

<p>Confocal microscopic images show YFP positive cells in non-ischemic brain 14 days after injection of tamoxifen (B and C) and YFP positive cells in ischemic striatum 30 days after middle cerebral artery occlusion (MCAO, D). An orthogonal view shows that several YFP immunoreactive cells (green) were nestin positive (red) in the anterior SVZ of the lateral ventricle of non-ischemic brain (C). A light microscopic image of hematoxylin and eosin (H&E) stained coronal section shows striatal ischemic lesion 30 days after MCAO (E). Bars = 100 µm for B, and D, 10 µm in C, and 100 µm in E. Blue color  =  cell nuclei. LV  =  lateral ventricle. D0 represents the day of MCAO.</p

NGF and PDGF-C protein levels.

<p>Representative images of double immunofluorescent staining show that NGF and PDGF-C immunoreactivity (B, C, E, F, red, arrows) was co-localized to S100 positive Schwann cells (A, D, green, arrows). Western blot analysis (G) shows NGF and PDGF-C levels in the sciatic nerve tissue and β-actin was used as an internal control. *p<0.05 and #p<0.05 versus the non-diabetic mouse (DM) and the diabetic mouse treated with saline (DB), respectively. n = 6/group. Bar = 50μm. DBTA = diabetic mouse treated with tadalafil.</p