Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

TCRN: A Two-Step Underwater Image Enhancement Network Based on Triple-Color Space Feature Reconstruction

The underwater images acquired by marine detectors inevitably suffer from quality degradation due to color distortion and the haze effect. Traditional methods are ineffective in removing haze, resulting in the residual haze being intensified during color correction and contrast enhancement operations. Recently, deep-learning-based approaches have achieved greatly improved performance. However, most existing networks focus on the characteristics of the RGB color space, while ignoring factors such as saturation and hue, which are more important to the human visual system. Considering the above research, we propose a two-step triple-color space feature fusion and reconstruction network (TCRN) for underwater image enhancement. Briefly, in the first step, we extract LAB, HSV, and RGB feature maps of the image via a parallel U-net-like network and introduce a dense pixel attention module (DPM) to filter the haze noise of the feature maps. In the second step, we first propose the utilization of fully connected layers to enhance the long-term dependence between high-dimensional features of different color spaces; then, a group structure is used to reconstruct specific spacial features. When applied to the UFO dataset, our method improved PSNR by 0.21% and SSIM by 0.1%, compared with the second-best method. Numerous experiments have shown that our TCRN brings competitive results compared with state-of-the-art methods in both qualitative and quantitative analyses

High-efficiency Bessel beam array generation by Huygens metasurfaces

Bessel beams have attracted considerable interest because of their unique non-diffractive, self-healing characteristics. Different approaches have been proposed to generate Bessel beams, such as using axicons, diffractive optical elements, composite holograms, or spatial light modulators. However, these approaches have suffered from limited numerical aperture, low efficiency, polarization-dependent properties, etc. Here, by utilizing dielectric Huygens metasurfaces as ultrathin, compact platforms by integrating the functionalities of Dammann gratings and axicons, we successfully demonstrate multiple Bessel beam generation with polarization-independent property. The number of two-dimensional arrays can be controlled flexibly, which can enhance information capacity with a total efficiency that can reach 66.36%. This method can have various applications, such as parallel laser fabrication, efficient optical tweezers, and optical communication