Original Table of Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool [9].

<p>Original Table of Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092874#pone.0092874-Whiting1" target="_blank">[9]</a>.</p

Results of per-lesion analysis of four studies.

<p>Per-lesion analysis of diagnostic performance of the light blue crest (LBC) sign under narrow band imaging with magnification endoscopy (NBI-ME) to diagnose gastric intestinal metaplasia(IM): (<b>a</b>) pooled sensitivity; (<b>b</b>) pooled specificity; (<b>c</b>) pooled positive likelihood ratio; (<b>d</b>) pooled negtive likelihood ratio; (<b>e</b>) symmetric receiver operator curve characteristic (SROC) curve and area under curve(AUC).</p

Characteristics of studies included in meta-analysis of accuracy of light blue crest signto diagnose gastric intestinal metaplasia.

<p>Characteristics of studies included in meta-analysis of accuracy of light blue crest signto diagnose gastric intestinal metaplasia.</p

Application of the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool to the five studies included in the meta-analysis.

<p>↑ low risk of bias.</p

Light blue crest sign.

<p>Light blue crest (LBC) appears as blue-whit lines visible on the epithelial surface under narrow band imaging with magnification endoscopy (NBI-ME) (original photo, Olympus GIF-H260Z, under 80×magnification).</p

Study selection flow diagram.

<p>Study selection flow diagram.</p

Origin of Triassic granites in central Hunan Province, South China: constraints from zircon U–Pb ages and Hf and O isotopes

<div><p>Triassic granites crop out extensively in central Hunan Province, South China. Representative granites include the Baimashan, Weishan, and Ziyunshan plutons. Lithologically, these granites mainly comprise biotite monzogranite, two-mica granites, hornblende-biotite granite, hornblende-biotite monzogranite, and garnet-muscovite granite. These granites have <i>in situ</i> zircon secondary ion mass spectrum U–Pb ages between 223.2 ± 3.3 and 209.3 ± 4.0 Ma, indicating that they likely formed predominantly in the Late Triassic. These granitic plutons have similar zircon Hf and O isotopic compositions, with <i>ε</i>_Hf(<i>t</i>) values of −0.8 to −9.0, two-stage depleted mantle model ages (<i>T</i>_DM2) of 1.81–1.31 Ga, and weighted mean of <i>δ</i>¹⁸O_Zrc values of 8.53 ± 0.58‰ to 9.12 ± 0.28‰. Combined with U–Pb dating and Hf isotopic data, the elevated and variable <i>δ</i>¹⁸O and <i>ε</i>_Hf(<i>t</i>) values of the individual granites indicate that these Triassic granites were likely produced by partial melting of upper Paleoproterozoic to lower Mesoproterozoic metasedimentary rocks and are S-type granites. The variable proportions of inherited zircons in certain samples with U–Pb ages of 627–992 Ma indicate the involvement of lower–middle Neoproterozoic crustal materials during magma crystallization through wall-rock contamination, which resulted in the wide range of isotopic compositions. Underplating of mantle-derived magma may have provided the thermal energy for partial melting of the upper Paleoproterozoic and lower Mesoproterozoic basements, thereby generating these late Triassic granites. The lack of positive <i>ε</i>_Hf(<i>t</i>) values and high <i>δ</i>¹⁸O_Zrc values indicate that the contribution of mantle-derived magmas to these granites may be insignificant.</p></div

DataSheet1_Evi5 is required for Xenopus limb and tail regeneration.pdf

Amphibians such as salamanders and the African clawed frog Xenopus are great models for regeneration studies because they can fully regenerate their lost organs. While axolotl can regenerate damaged organs throughout its lifetime, Xenopus has a limited regeneration capacity after metamorphosis. The ecotropic viral integrative factor 5 (Evi5) is of great interest because its expression is highly upregulated in the limb blastema of axolotls, but remains unchanged in the fibroblastema of post-metamorphic frogs. Yet, its role in regeneration-competent contexts in Xenopus has not been fully analyzed. Here we show that Evi5 is upregulated in Xenopus tadpoles after limb and tail amputation, as in axolotls. Down-regulation of Evi5 with morpholino antisense oligos (Mo) impairs limb development and limb blastema formation in Xenopus tadpoles. Mechanistically, we show that Evi5 knockdown significantly reduces proliferation of limb blastema cells and causes apoptosis, blocking the formation of regeneration blastema. RNA-sequencing analysis reveals that in addition to reduced PDGFα and TGFβ signaling pathways that are required for regeneration, evi5 Mo downregulates lysine demethylases Kdm6b and Kdm7a. And knockdown of Kdm6b or Kdm7a causes defective limb regeneration. Evi5 knockdown also impedes tail regeneration in Xenopus tadpoles and axolotl larvae, suggesting a conserved function of Evi5 in appendage regeneration. Thus, our results demonstrate that Evi5 plays a critical role in appendage regeneration in amphibians.</p

RAD6 regulates cell proliferation and tumor growth in vitro.

<p>(<b>A</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 plasmids, or a control siRNA or RAD6 specific siRNAs were subjected to MTT assay. OD570 value was examined for the analysis of cell proliferation. Five replicates of each treatment were employed in this assay (n = 5). (<b>B</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 plasmids were subjected to soft agar colony formation assay. The quantification of the colony number is shown below. Three replicates of each treatmen were employed in this assay (n = 3). (<b>C</b>) HL-7702 cells transfected with control or RAD6 shRNAs were subjected to soft agar colony formation assay. The quantification of the colony number is shown on the right. Three replicates of each treatmen were employed in this assay (n = 3).</p

RAD6 regulates G1-S transition in human cells.

<p>(<b>A</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 plasmids were stained with propidium iodide (PI) and subjected to cell cycle analysis. The quantification of the cell cycle distribution is shown below. The percentage of each phase cells was employed for the analysis of cell cycle distribution. (<b>B</b>) HL-7702 cells transfected with control or RAD6 siRNAs were stained with PI and subjected to cell cycle analysis. The quantification of the cell cycle distribution is shown on the right. The percentage of each phase cells was employed for the analysis of cell cycle distribution. (<b>C</b>) The expression levels of RAD6 in cells used in the above cell cycle assays (overexpression, upper; knockdown, lower) were analyzed by western blot assay.</p