A case of placenta previa accreta successfully treated by open infrarenal aortic clamping

Open infrarenal aortic clamping has been performed for traumatic pelvic hemorrhage or aortic rupture, and there are few reports of its use in the field of obstetrics and gynecology1). We report a patient with placenta previa accreta who was successfully treated by open infrarenal aortic clamping

Study on the effect of space radiation to mammalian ES and iPS cells to their development

It is extremely important to take measure the effects of space radiation on the human body to forecast and defend them. Especially, reproduction and development in space will be an issue of high priority considering a long-term stay in space. We will launch frozen mouse ES cells and iPS cells into space, to expose them to space radiation. After storage at -80 degree in C in space for several months or years, the cells will be returned to the ground. We will microinject the ES or iPS cells into fertilized eggs to produce mice to examine the influence of space radiation on their development and on their descendents.We used iron ion and carbon ion beam by accelerator of HIMAC of National Institute of Radiological Sciences as a ground-based experiment. The embryonic stem cells and iPS cells were irradiated with those beams and microinjected to mouse embryos, and subsequently transplanted to the uterus of pseudopregnant mice. We analyzed their survival, proliferation, and development.21st Annual NASA Space Radiation Investigators’ Worksho

Effect of heavy ion radiation to mouse ES cells

When a long term manned space flight and stay is assumed, it is considered to be one of the most prime issues to evaluate the influence of the space radiation on the human body. Especially damages occurring in the reproductive organs bear the risk to seriously injure the descendant. Because experiments using breeding mouse in space is difficult, we propose an alternative method to evaluate the effect of space radiation to mammals. We plan to launch frozen embryonic stem (ES) cells in space for being exposed to space radiation. After returns to ground, the cells will be microinjected to mouse blastocysts to become chimeric mice. The process of development of ES cells can be followed to judge the radiation effects.We carried out irradiation experiment with heavy particles on ground at HIMAC at Chiba in Japan. We irradiated mouse cultured ES cells after irradiation with doses from 0.01 to 10Gy with carbon (290 MeV/u) and iron ions (500 MeV/u) at room temperature. The survival rate of the cell was analyzed by colony formation. The results show an increase in response in the following order: Fe>C>X-rays. It is planned to launch frozen ES cell; accordingly in a ground-based experiment mouse ES cell frozen at -80 degree-C were also irradiated by dose from 0.01 to 10Gy with carbon (290 MeV/u) and iron ion (500 MeV/u) beams. The number of colonies was counted eight days later and the survival rate of the cells was analyzed. For the dose of 1Gy or more, cells irradiated at standard culture condition are more sensitive than frozen cells.We irradiated the mouse ES cells by the dose to 0.01-10Gy with carbon (290 MeV/u) and iron (500 MeV/u) beams, and the histone H2AX (gamma-H2AX) phosphorylation was followed. The gamma-H2AX was strongly detected at doses greater than 0.5 Gy 1hr after irradiation, while foci formation after X-ray irradiation was moderately, indicating the induction of severer DNA damage by iron beam irradiation. We will evaluate the influence of space radiation by analyzing development of ES cells-microinjected embryos. The system worked well in the experiment with X-rays, showing the generation of an abnormal embryo to be proportional to irradiation dose. In addition, we try to estimate the influence of space radiation on human ontogeny by comparing the effect to human and mouse iPS cells by using microinjection system.Heavy Ions in Therapy and Space Symposium 200

Study of the Effects of Space Radiation on Mouse ES cells

As long-term human space flight is now required for cosmic exploration, the influence of space radiation and microgravity on the human body is an issue of high priority. We plan to launch frozen mouse embryonic stem (ES) cells into space, to expose them to space radiation. After returning to the ground, we will microinject the ES cells into fertilized eggs to produce mice, and evaluate the influence of space radiation on their development and on their descendents