Determination of LC50 value of Nicotiana tabacum L. extract against Tenebrio molitor and Zophobas morio larvae

Tobacco has lots of active compounds that can be used as raw material for making natural insecticides. Tenebrio molitor and Zophobas morio are examples of agricultural pests. The purpose of this study was to determine LC50 values of tobacco extract on Tenebrio molitor and Zophobas morio. Previous research showed that tobacco extract had neurotoxin activity and nicotine was the highest content contained in the tobacco leaves. Tobacco extract was obtained by the Extended Heat Reflux Extraction method with ethanol solvent. LC50 values of tobacco extract for Tenebrio molitor and Zophobas morio were 21.1 mg/ml and 71.1 mg/ml, respectively

Determination of LC50 value of Nicotiana tabacum L. extract against Gryllus bimaculatus imago and Galleria mellonella larvae

Replacement of synthetic pesticide with natural pesticide is highly demanding because it is more environmentally friendly. Tobacco is majorly exploited for cigarettes production. Therefore, it needs to be developed for other alternative products, one of which is insecticide due to nicotine content and other toxic compounds. This research aimed to determine the effectiveness of tobacco as an insecticide for Galleria mellonella and Gryllus bimaculatus. The raw material used in this study was the leaf extract of Nicotiana tabacum L., var. Virginia that was obtained by Ethanolic Heat Reflux Extraction (EHRE) technique. After testing on the insects, LC50 values were 36.6 mg/ml for Galleria mellonella and 38.5 mg/ml for Gryllus bimaculatus