A taxonomic evalution of Alburnus sellal heckel, 1843 and alburnus adanensis Battalgazi, 1944: Based on morphological characters and mitochondrial DNA sequences

Alburnus sellal inhabits Ceyhan, Euphrates, Orontes and Tigris river basins whereas Alburnus adanensis is geographically restricted to Ceyhan and Seyhan river basins. Due to the very similar morphometric and meristic characteristics, discrimination of these two species is difficult. The most important diagnostic character is the scale numbers in lateral line (73-80 in A. sellal vs 60-66 in A. adanensis) and pronounced lack of the dark grey band on side of the body. In order to clarify the taxonomic status of the A. sellal and A. adanensis, a total of 27 morphological characters and the nucleotide sequence variation of mitochondrial cytochrome b (cyt b) and cytochrome oxidase I (COI) genes were analyzed on the specimens which were from Seyhan, Ceyhan, lower Euphrates and Orontes river basins. The results show high similarity between A. sellal and A. adanensis based on the morphometric and the morphological characters using Principal Component Analyses (PCA). In addition genetic distance of the specimens belonging to two species between 0.000 to 0.011 using COI and cyt b genes sequences. Therefore, our results based on morphology, molecules and nomenclatural priority pointed out that A. adanensis should be evaluated as a synonym of A. sellal.Nevsehir Hacı Bektas Veli University, Scientific Research Project

Seroprevalance of Brucellosis, Listeriosis and Toxoplasmosis in cattle in Adana province of Turkey

Amaç: Bu çalışma, Nisan 2008-Eylül 2008 tarihleri arasında Adana'da farklı yaş ve cinsiyetteki sağlıklı Holstein melezi sığırlarda zoonoz karakterde olan ve abort yapan hastalıklardan toksoplazmozis, listeriozis ve brusellozis'in seroprevalansının belirlenmesi amacı ile yapıldı.Yöntemler: Bu amaçla,132 sığıra ait kan serumu Toxoplasma gondii, Listeria monocytogenes ve Brucella abortus antikorları yönünden incelendi. Serumlarda T. gondii antikorları standart Sabin-Feldman Dye Testi (SFDT), L. monocytogenes ''O'' antikorları Osebold yöntemi, B. abortus antikorları ise Mikrotüp Aglutinasyon Testi (MAT) ile tespit edildi.Bulgular: Test edilen 132 kan serum örneğinden %56,06'sının T. gondii, %40,9'unun L. monocytogenes ve %3,03'ünün B. abortus antikorları yönünden seropozitif olduğu saptandı. Toxoplasma gondii, L. monocytogenes ve B. abortus antikorlarının seropozitiflik yönünden yaş gruplarına göre dağılımında istatistiki bir farkın olmadığı belirlendi (p>0,05). Sonuç: Bu çalışma ile Adana yöresinde sığırlarda ilk kez serolojik yöntemlerle L. monocytogenes varlığı ortaya konuldu. Toksoplazmozis seropozitifliği, listeriozis ve brucellozise göre yüksek bulundu. Ayrıca, bu hastalıkların aynı hayvanda birlikte görülme sıklığı en yüksek oranda T. gondii ve L. monocytogenes için tespit edildi.Objective: This study was conducted to identify the seroprevalance of diseases which are zoonotic and responsible from abortion such as toxoplasmosis, listeriosis and brucellosis in Holstein crossbred cattle of different age and sex in Adana province, between 2008 April-September.Methods: For this purpose, blood serum samples were collected from 132 cattle and analyzed for Toxoplasma gondii, Listeria monocytogenes and Brucella abortus antibodies. T. gondii, L. monocytogenes and Brucella abortus antibodies were determined by the standard Sabin- Feldman Dye Test (SFDT), Osebold method and Microtube Agglutination Test (MAT) respectively, from the blood serum samples.Results: 132 serum tested 56.06% samples of T. gondii, 40.9% and 3.03% of L. monocytogenes and defined the B.abortus antibodies were found to be seropositive terms. There were no statistically significant difference between seropositive T. gondii, L. monocytogenes and B.abortus antibodies among age groups (p>0.05).Conclusion: In this study, for the first time in cattle in the region of Adana serological methods revealed the presence L. monocytogenes, toxoplasmosis and listeriosis were higher than brucellosis seropositivity. Moreover, the prevalence of these diseases in the same animal at the highest rate was determined for T. gondii and L. monocytogenes