Genome-Wide Association Study and Pathway-Level Analysis of Kernel Color in Maize.

Rapid development and adoption of biofortified, provitamin A-dense orange maize (Zea mays L.) varieties could be facilitated by a greater understanding of the natural variation underlying kernel color, including as it relates to carotenoid biosynthesis and retention in maize grain. Greater abundance of carotenoids in maize kernels is generally accompanied by deeper orange color, useful for distinguishing provitamin A-dense varieties to consumers. While kernel color can be scored and selected with high-throughput, low-cost phenotypic methods within breeding selection programs, it remains to be well established as to what would be the logical genetic loci to target for selection for kernel color. We conducted a genome-wide association study of maize kernel color, as determined by colorimetry, in 1,651 yellow and orange inbreds from the Ames maize inbred panel. Associations were found with y1, encoding the first committed step in carotenoid biosynthesis, and with dxs2, which encodes the enzyme responsible for the first committed step in the biosynthesis of the isoprenoid precursors of carotenoids. These genes logically could contribute to overall carotenoid abundance and thus kernel color. The lcyE and zep1 genes, which can affect carotenoid composition, were also found to be associated with colorimeter values. A pathway-level analysis, focused on genes with a priori evidence of involvement in carotenoid biosynthesis and retention, revealed associations for dxs3 and dmes1, involved in isoprenoid biosynthesis; ps1 and vp5, within the core carotenoid pathway; and vp14, involved in cleavage of carotenoids. Collectively, these identified genes appear relevant to the accumulation of kernel color

Social stratification and tooth loss among middle‐aged and older Americans from 1988 to 2004

Objectives Tooth retention has improved over the past few decades, but it is not known whether these trends have been observed across all demographic/socioeconomic subgroups. We examined number of missing teeth among dentate individuals ( n  = 9, 113) as well as edentulism and systematically modeled their trends over time by using clinical examination data. Methods We investigated the association between social stratification and trends in tooth retention among adults ages 50+ from 1988 to 2004, using four waves of the National Health and Nutrition Examination Surveys ( NHANES ) ( n  = 11 812). Results The prevalence of edentulism declined from 24.6% in NHANES III (1988–1994) to 17.4% in 2003–2004, and the mean number of missing teeth declined from 8.19 to 6.50. Older participants, Blacks, the less educated and those with lower income were higher on both edentulism and number missing teeth. Both edentulism and number of missing teeth declined over time, but their patterns varied. For edentulism, age and socioeconomic related disparities decreased over time due to more decline among older and low‐income participants. For missing teeth, there was less decrement among older and low‐income participants, resulting in increased age and socioeconomic related disparities. Conclusions Our study found disparities in trends of tooth loss across demographic/socioeconomic strata. Findings suggest that racial/ethnic disparities are partially explained by socioeconomic status. Interventions designed to improve oral health for older adults, particularly those with low levels of income, need special attention.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/109563/1/cdoe12116.pd

P1‐100: Cognitive function and oral hygiene behavior in later life

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152607/1/alzjjalz201205376.pd

Modeling the Distribution of Organic Coffee Supply Chain from Junín Region, Peru

This research uses Supply Chain (SC) data gathered during the distribution and transportation activities of organic coffee. The purpose of this research is to analyze different ways in which crop-chain solutions are adopted in the region of Junn, Peru. The disintegration of the SC is revealed, as each cooperative member works uncoordinatedly on his own leading to different results standards with high costs and low returns. The study focused on different entities from farmers to the port of Callao of an organic coffee supply chain where disintegration is constant. The paper describes a misaligned supply chain including poorly collaboration. All problems found will be an opportunity to develop a distribution model in the organic coffee supply chain of Junn region

Edentulism trends among middle‐aged and older adults in the United States: comparison of five racial/ethnic groups

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90571/1/j.1600-0528.2011.00640.x.pd

Oral health among white, black, and Mexican‐American elders: an examination of edentulism and dental caries

Objectives: To examine racial/ethnic disparities in oral health among older Americans. Methods: Differences in frequency of edentulism and number of decayed, missing, and filled teeth were assessed in 2,679 non‐Hispanic white, 742 non‐Hispanic black, and 934 Mexican‐American individuals aged 60 and older from the National Health and Nutrition Examination Survey (1999‐2004). Results: Controlling for potential confounding variables, blacks and Mexican‐Americans had significantly higher numbers of decayed teeth but fewer numbers of filled teeth than whites. Although blacks had a lower likelihood of being edentulous than whites, dentate blacks had a higher number of missing teeth. Compared with whites, Mexican‐Americans were less likely to be edentulous, and dentate Mexican‐Americans had fewer missing teeth. Our study also showed that blacks and Mexican‐Americans had less frequent dental checkups than whites. Conclusions: Oral health disparities are persistent across racial/ethnic groups for older Americans despite the fact that the differences between groups typically diminish when socioeconomic, health‐related, and behavioral factors are considered in the models. Our study suggests that reducing racial/ethnic oral health disparities requires multiple clinical approaches.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88103/1/j.1752-7325.2011.00273.x.pd

Trends in decayed teeth among middle‐aged and older adults in the United States: socioeconomic disparities persist over time

ObjectivesWhile trends in tooth loss among older adults have been well documented and show a decline over the last few decades, little is known about trends in tooth decay which may lead to tooth loss. The study aim was to examine trends in tooth decay among adults ages 50 years and older in the United States and determine whether these trends were consistent across demographic and socioeconomic subgroups of middle‐aged and older adults.MethodsSecondary analysis of data collected through detailed oral health examinations in the National Health and Nutrition Examination (NHANES) surveys 1988–1994 and 1999–2004. Tooth decay was measured as active caries. Multivariable associations were estimated using negative binomial regression models.ResultsAveraged over time, the mean number of decayed teeth was 0.54. Rates of decay remained stable over time. Males, non‐Hispanic Blacks, Mexican‐Americans, and those of other race/ethnicity as well as those with fewer years of education and lower levels of income had more decayed teeth. The increased number of decayed teeth for Mexican‐Americans and those of other race/ethnicity was due in part to differing levels of education and income. Trends over time did not vary by any of these demographic and socioeconomic characteristics. Trends in the number of decayed teeth did not meaningfully change when the numbers of missing and filled teeth were controlled.ConclusionsAlthough studies have shown the number of middle‐aged and older Americans experiencing tooth loss has decreased over time, trends in tooth decay have remained relatively stable, with socioeconomic disparities persisting over time.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135439/1/jphd12153_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135439/2/jphd12153.pd

Hypothalamic mitochondria in energy homeostasis and obesity

Citation: Guo X, Wu L, Wang W, Medeiros DM, Clarke S, et al.(2016) Hypothalamic mitochondria in energy homeostasis and obesity. Integr Mol Med 3: DOI: 10.15761/IMM.1000209.Obesity, which is largely due to energy imbalance, has emerged as one of the most serious health issues in the world. The hypothalamus is the most important organ to regulate feeding behavior and energy expenditure through nutrient sensing and signal integration from central and peripheral pathways. As the main organelle to produce energy, mitochondria play a critical role in energy homeostasis from the organelle level. Besides providing a platform for the oxidation of fuel substrates, mitochondria are also involved in a variety of cell signaling pathways and modulate energy homeostasis through mitochondrial dynamics. Mitochondrial dysfunction may lead to obesity due to inadequate ATP production, oxidative stress, endoplasmic reticulum stress, and inflammation. ?, ?-carotene-9’,10’-oxygenase2 (BCO2) is a mitochondrial enzyme that catalyzes the asymmetric cleavage of both provitamin A and non-provitamin A carotenoids. This enzyme is localized to the inner mitochondrial membrane, where the electron transport chain is located. Besides the enzymatic function, BCO2 is important for mitochondrial function and is genetically associated with interleukin-18. Moreover, BCO2 protein expression is suppressed in obese and diabetic mice. Given that the important role of BCO2 in mitochondrial structure and function, and the key position of the hypothalamus in energy balance, BCO2 may play a new role in maintaining metabolic homeostasis that has been overlooked before. The mutation of BCO2 might lead to the impairment of whole body energy homeostasis through hypothalamic mitochondrial dysfunction. Here we will be presenting the updates on hypothalamic mitochondria in cellular energy homeostasis and discussing the potential of BCO2 in regulation of hypothalamic mitochondria in health and obesity.