Studi Tingkat Penyalahgunaan Narkoba pada Mahasiswa di Denpasar dan Badung

The level of knowledge and their abuses of drugs among students in some university in Denpasar and Badung regency have been assessed. Eight hundred students have been integrated on survey of knowledge-risk - effects survey and 2085 students were screened for drugs abused. Aim of this study was to assets the drugs knowledge level of students and determine their abuser level uncorrelated to their risk. We found out that, all students have ever attended sort course on drugs abuses and 85 % of student's active searched drugs information through internet. On the contrary was obtained the low level (28-36%) of knowledge-risk-effect on drugs. About the 34% of 800 respondents presented high risk to abused drugs. Surprisingly it was found out just one among 2085 students positive consume codeine after screening and determinations tests

Amplifikasi Fragmen Gen 18s Rrna Pada Dna Metagenomik Madu Dengan Teknik Pcr (Polymerase Chain Reaction)

The aim of this research was to amplificate 18S rRNA gene fragment from honey's metagenomic DNA using Polymerase Chain Reaction (PCR). The honey sample was collected from Seraya Tengah village, Karangasem regency. The best result of primer design from in silico test was continued to in vitro test using PCR method. The optimum conditions for amplification was obtained as follows: pre-denaturation at 95oC for 3 minutes and continued with 30 of amplification cycle (denaturation at 95°C for 1 minutes, annealing at 55°C for 1 minutes and elongation at 72°C for 1 minutes) and the last step continued with extension process at 72°C for 2 minutes. The size of DNA fragment band of amplified product was about 100 bp which obtained from the honey's metagenomic DNA

Perbandingan Kualitas Dna Dengan Menggunakan Dua Metode Boom Modifikasi Pada Isolat Mycobacterium Tuberculosisp10 Di Bali

Isolasi DNA bakteri merupakan salah satu metode penunjang yang dapat digunakan untuk mendeteksi bakteri penyebab infeksi. Metode yang umum digunakan dalam mengisolasi DNA Mycobacterium tuberculosis adalah metode Boom. Metode ini masih perlu mengalami modifikasi agar pelepasan DNA menjadi lebih optimal serta mengurangi inhibitor pada proses PCR. Tujuan dari penelitian ini adalah untuk membandingkan kualitas DNA yang dihasilkan dari masing-masing metode Boom modifikasi dalam mengisolasi DNA M. tuberculosis. Hasil isolasi ini nantinya akan diamplifikasi dengan teknik PCR sehingga bakteri dapat terdeteksi dengan cepat.Visualisasi produk PCR akan dianalisis dengan menggunakan 1,5 % gel agarosa. Dalam penelitian ini, dibandingkan 2 metode Boom yang telah dimodifikasi. Hasil yang diperoleh diketahui bahwa pita DNA produk PCR terlihat tebal pada masing-masing metode. Kemudian dilanjutkan analisis untuk mengetahui ketebalan pita yang dihasilkan dengan menghitung area pita. Hasil yang diperoleh diketahui bahwa metode Boom modifikasi yang dikerjakan di Laboratorium Biomolekular FK Unud lebih baik dibandingkan dengan metode Boom modifikasi yang dikerjakan oleh Traore

Desain Primer Secara in Silico Untuk Amplifikasi Fragmen Gen Rpob Mycobacterium Tuberculosis Dengan Polymerase Chain Reaction (Pcr)

Amplifikasi DNA Mycobacterium tuberculosis dari gen rpoB dilakukan dengan metode polymerase chain reaction (PCR). Amplifikasi DNA dengan PCR diperlukan sepasang primer (forward dan reverse) untuk membatasi daerah yang ingin diamplifikasi. Penelitian ini bertujuan untuk mendesain sepasang primer agar dapat mengamplifikasi fragmen 0,5 kb gen rpoB M.tuberculosis. Desain primer dilakukan secara in silico dengan bantuan program clone manager suite 6 (University of Groningen). Template yang digunakan dalam mendesain primer adalah sekuen gen rpoB M. tuberculosis H37RV wild type, yang diperoleh dari database NCBI dengan kode genbank U12205.1. Penelitian ini telah berhasil memperoleh sekuen sepasang primer (forward dan reverse) dengan panjang masing-masing adalah 22 oligonukleotida. Primer ini dapat mengamplifikasi secara in silico fragmen 0,5 kb gen rpoB M. tuberculosis pada rentang daerah 990-1496 pb dengan panjang fragmen sebesar 507 pb

PERBANDINGAN METODE UJI GULA PEREDUKSI DALAM PENENTUAN AKTIVITAS ?-L-ARABINOFURANOSIDASE DENGAN SUBSTRAT JANUR KELAPA (COCOS NUCIFERA)

Pengujian  gula pereduksi umumnya dilakukan dengan reagen asam 2,3-dinitrosalisilat (DNS) dan Nelson-Somogyi (NS) dalam penentuan aktivitas enzim pendegradasi polisakarida. Tujuan penelitian ini adalah untuk membandingkan metode uji gula pereduksi DNS dan NS dalam penentuan aktivitas ?-L-Arabinofuranosidase (AbfA) termostabil, dengan substrat janur kelapa (Cocos nucifera). Enzim AbfA diperoleh dari Saccharomyces cerevisiae rekombinan yang dikultivasi selama 3 hari pada suhu inkubasi 30 °C.  Penentuan aktivitas enzim AbfA dilakukan pada kondisi pH 6, suhu 70 °C dan waktu inkubasi 15 menit. Hasil penelitian menunjukkan bahwa penentuan aktivitas enzim AbfA dengan metode NS lebih teliti atau memiliki standar deviasi yang jauh lebih kecil dibandingkan dengan metode DNS, namun kurva kalibrasi larutan standar metode DNS lebih linear dibandingkan  metode NS

Optimization of Annealing Temperature for Amplification of 507 bp fragment of rpoB Gene of Clinical Multidrug-Resistant Mycobacterium tuberculosis Isolate 86

The gene of rpoB is the primary gene that is well known as a surrogate marker in MDR-TB (Multidrug-Resistant Tuberculosis) detection. The mutation of rpoB was studied around the world in its core region called RRDR (Rifampicin Resistance Determining Region). To learn the mutation in this fragment, PCR (Polymerase Chain Reaction) is the most common method used. This study was purposed to optimize the annealing temperature in amplifying the 507 bp fragment of rpob gene containing RRDR of isolate 86.DNA of MDR-TB isolate 86 was isolated by using Boom method for further amplified by PCR. The oligonucleotide primers used in this study were FrTB and RrTB. Eight different annealing temperature were used to optimize the amplification of rpoB gene : 53, 55, 57, 58, 59, 60, 61, and 63oC. Detection of PCR products was done with 1,5% agarose gel electrophoresis.Agarose gel electrophoresis of PCR products showed that 507 bp of rpoB gene could be produced at all annealing temperatures. A faint amplified product was observed at temperature 53 and 55oC. However, at temperature 58 and 60oC more intense bands were observed. In conclusion, the best annealing temperature was at 60oC in producing the 507 bp fragment

SUHU DAN WAKTU OPTIMUM PROSES EKSTRAKSI ANTOSIANIN DALAM UBI JALAR UNGU (Ipomoea batatas L.) DENGAN ?-L-ARABINOFURANOSIDASE

Enzyme-assisted extraction (EAE) method is one of the most environmentally friendly methods of enzyme application in the extraction of bioactive compounds. The purpose of this study was to determine the optimum temperature and time required in the extraction of anthocyanin compounds from purple sweet potato (Ipomoea batatas L.) with and without ?-L-arabinofuranosidase (AbfA) - assisted. The AbfA enzyme was obtained from Saccharomyces cerevisiae recombinant strain BJ1824 contain pYHMI-Af plasmid. The optimum temperature and time in the extraction of anthocyanin compound with and without  ?-L-arabinofuranosidase  from purple sweet potato were performed on the 40, 50, 60 and 700C; and 150, 180, 210 minutes. The extraction was done by ethanol solvent of 60,32% (v/v) acidified with citric acid of 2,39% (b/v). The measurement of anthocyanin levels using UV-Vis Spectrophotometer at 527 nm and 700 nm wavelengths at pH 1,0 and 4,5. The optimum condition of non-enzyme-assisted extraction was at 600C for 210 minutes, with the anthocyanin levels of 26,3842 mg/L; while with the AbfA enzyme-assisted at 500C for 180 minutes, with the anthocyanin levels of 28,2056 mg/L. The extraction with enzyme-assisted resulted the anthocyanin levels of 6,90% higher than without the using of enzyme

Anti-inflammatory Activity of Andong Leaf Extract (Cordyline Terminalis Kunth) Against Edema in the Soles of Wistar Rats

The Balinese plant andong (Cordyline terminalis Kunth) has anti-inflammatory properties. The focus of this research was to determine whether andong leaf extract has anti-inflammatory properties and is effective against carrageenan-induced edema in rat soles. In this study, carrageenan was used to induce inflammation in the soles of Wistar rats. 25 rats were divided into five treatment groups. Group I was given aquadest as the control; group II was given diclofenac sodium at a dose of 4.5 mg/kgbw as a drug control; and groups III, IV and V were given ethanol extract of andong leaves at doses of 150 mg/kgbw, 300 mg/kgbw, and 600 mg/kgbw orally as test groups. For six hours, the inflammation volume was measured using a plethymometer. One-way ANOVA was used to test differences between the treatment groups. The results showed that the ethanol extract of andong leaves had the highest anti-inflammatory activity in the six-hour period when given at doses of 150 mg/kgbw (36.35% 0.02), 300 mg/kgbw (26.30% 0.20), and 600 mg/kgbw (20.67% 0.16) with a significant difference (p < 0.05) compared to the negative control (86.84% 0.092), but this was not significantly different from the positive control (22.21% 0.01). Based on these findings, it can be concluded that a dose of 600 mg/kgbw is the most effective in reducing inflammation. Keywords: Anti-inflammatory, Red andong leaf, Carrageenan, Wistar rat, Edem