A figure of merit measuring picture resolution

Figure of merit measuring picture resolutio

Five day mission plan to investigate the geology of the Marius Hills region of the moon

Five-day mission plan to investigate geology of Marius Hills region of moo

The lunar reflectivity model for ranger block iii analysis

Lunar reflectivity model for Ranger Block III ANALYSI

Ranger VII photographic parameters

Tables of computer generated photographic parameters necessary for detailed interpretation of Ranger VII television camera pictures - trajectory and time-to-impact dat

Mission to a comet - Preliminary scientific objectives and experiments for use in advanced mission studies

Scientific objectives and experiments for comet missio

The Relationship of Implicit Theories to Elementary Teachers’ Patterns of Engagement in a Mathematics-Focused Professional Development Setting

As elementary teachers aim to deepen their mathematical understandings, they engage in a relearning process that involves not only revisiting but also reconstructing their knowledge. To do so, meaningful engagement in immersion and practice-based experiences is required. This exploratory case study investigated the engagement patterns of two elementary teachers with varying implicit beliefs as they participated in a professional development that focused on relearning mathematics. Data were collected on the two participants in the form of video narratives, observation protocols, and interviews. Attention was given to their patterns of engagement in collaborative group settings as the participants moved through different phases of the professional development lesson. Results indicated that the engagement patterns of the two participants closely aligned with learning behaviors described in the implicit beliefs theory. In this way, the results suggested an extension of the implicit theories model to the relearning context. Additional implications and future questions are provided

A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins

We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template to make the first strand of a cDNA. Then a second strand was synthesized and amplified by using two sets of DNA primers that (i) hybridized to the ends of the light- and heavy-chain variable regions, (ii) encoded a linker peptide, and (iii) contained appropriate restriction enzyme sites for cloning. After 30 cycles of PCR, the DNA fragments containing sequences encoding the light- and heavy-chain variable regions were cloned into an Escherichia coli expression vector containing a portion of the Pseudomonas exotoxin gene. Clones encoding recombinant single-chain immunotoxins were expressed in E. coli and the protein product was assessed for its ability to bind to or kill cells bearing the OVB3 antigen. By using this approach it should be possible to rapidly clone the functional variable region sequences of many different antibodies from hybridoma RNA