10,710 research outputs found
Characterization of theThreshold for NAD(P)H:quinone Oxidoreductase Activity in Intact Sulforaphane-treated Pulmonary Arterial Endothelial Cells
Treatment of bovine pulmonary arterial endothelial cells in culture with the phase II enzyme inducer sulforaphane (5 μM, 24 h; sulf-treated) increased cell-lysate NAD(P)H:quinone oxidoreductase (NQO1) activity by 5.7 ± 0.6 (mean ± SEM)-fold, but intact-cell NQO1 activity by only 2.8 ± 0.1-fold compared to control cells. To evaluate the hypothesis that the threshold for sulforaphane-induced intact-cell NQO1 activity reflects a limitation in the capacity to supply NADPH at a sufficient rate to drive all the induced NQO1 to its maximum activity, total KOH-extractable pyridine nucleotides were measured in cells treated with duroquinone to stimulate maximal NQO1 activity. NQO1 activation increased NADP+ in control and sulf-treated cells, with the effect more pronounced in the sulf-treated cells, in which the NADPH was also decreased. Glucose-6-phosphate dehydrogenase (G-6-PDH) inhibition partially blocked NQO1 activity in control and sulf-treated cells, but G-6-PDH overexpression via transient transfection with the human cDNA alleviated neither the restriction on intact sulf-treated cell NQO1 activity nor the impact on the NADPH/NADP+ ratios. Intracellular ATP levels were not affected by NQO1 activation in control or sulf-treated cells. An increased dependence on extracellular glucose and a rightward shift in the Km for extracellular glucose were observed in NQO1-stimulated sulf-treated vs control cells. The data suggest that glucose transport in the sulf-treated cells may be insufficient to support the increased metabolic demand for pentose phosphate pathway-generated NADPH as an explanation for the NQO1 threshold
Multiband processing of multimode light: combining 3D photonic lanterns with waveguide Bragg gratings
The first demonstration of narrowband spectral filtering of multimode light
on a 3D integrated photonic chip using photonic lanterns and waveguide Bragg
gratings is reported. The photonic lanterns with multi-notch waveguide Bragg
gratings were fabricated using the femtosecond direct-write technique in
boro-aluminosilicate glass (Corning, Eagle 2000). Transmission dips of up to 5
dB were measured in both photonic lanterns and reference single-mode waveguides
with 10.4-mm-long gratings. The result demonstrates efficient and symmetrical
performance of each of the gratings in the photonic lantern. Such devices will
be beneficial to space-division multiplexed communication systems as well as
for units for astronomical instrumentation for suppression of the atmospheric
telluric emission from OH lines.Comment: 5 pages, 4 figures, accepted to Laser & Photonics Review
Glueball enhancements in p(gamma,VV)p through vector meson dominance
Double vector meson photoproduction, p(gamma, G -> VV)p, mediated by a scalar
glueball G is investigated. Using vector meson dominance (VMD) and
Regge/pomeron phenomenology, a measureable glueball enhancement is predicted in
the invariant VV = rho rho and omega omega mass spectra. The scalar glueball is
assumed to be the lightest physical state on the daughter pomeron trajectory
governing diffractive vector meson photoproduction. In addition to cross
sections, calculations for hadronic and electromagnetic glueball decays, G -> V
V' (V,V'= rho, omega, phi, gamma), and gamma_v V -> G transition form factors
are presented based upon flavor universality, VMD and phenomenological
couplings from phi photoproduction analyses. The predicted glueball decay
widths are similar to an independent theoretical study. A novel signature for
glueball detection is also discussed
Leg Injuries To Coyotes Captured In Standard And Modified Soft Catch® Traps
Leg injuries of coyotes (Canis latrans) captured in standard No. 3 Soft Catch traps were compared with those captured in the same trap type modified with two additional coil springs. One hundred thirteen coyotes were trapped in southern California in conjunction with livestock predator control operations, 53 in standard traps, and 60 in modified traps. Observed injuries were similar in both trap types. The most frequent injuries were edematous hemorrhages and small cutaneous lacerations. Injuries, such as joint luxations and bone fractures, were noted more frequently for coyotes trapped in standard Soft Catch traps
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