64 research outputs found
Noise Minimization in Cell-Free Gene Expression
Biochemical reactions that involve small numbers of molecules are accompanied by a degree of inherent randomness that results in noisy reaction outcomes. In synthetic biology, the ability to minimize noise particularly during the reconstitution of future synthetic protocells is an outstanding challenge to secure robust and reproducible behavior. Here we show that by encapsulation of a bacterial cell-free gene expression system in water-in-oil droplets, in vitro-synthesized MazF reduces cell-free gene expression noise >2-fold. With stochastic simulations we identify that this noise minimization acts through both increased degradation and the autoregulatory feedback of MazF. Specifically, we find that the expression of MazF enhances the degradation rate of mRNA up to 18-fold in a sequence-dependent manner. This sequence specificity of MazF would allow targeted noise control, making it ideal to integrate into synthetic gene networks. Therefore, including MazF production in synthetic biology can significantly minimize gene expression noise, impacting future design principles of more complex cell-free gene circuits.</p
University of Nebraska Five-Year Strategy, Revised August 12, 2020
The University of Nebraska Five-Year Strategy: Trust, Predictability, and Positive Outcomes for Nebraskans
In February 2020, the newly named president of the University of Nebraska system, Ted Carter, gathered a diverse 28-member team of students, faculty, staff, and administrators to help chart the path forward for Nebraska’s public university. The team’s goal: At a time of great change in higher education, lay out a vision for what the future should look like for the University of Nebraska.
Broad themes quickly emerged, including student access and success, excellence in teaching and research, diversity and inclusion, partnerships, and fiscal effectiveness. Then COVID-19 hit, forcing a pause in the team’s work. The ensuing months showed that the initial priorities identified by the team were not only still relevant, but more important than ever in defining the future of higher education.
From that early work has emerged a five-year strategy for growth and success across the four-campus University of Nebraska system. In addition to the strategic planning team, Carter engaged alumni and donors, elected leaders, leaders in business and agriculture, the Board of Regents, NU senior leadership, and others in conversations about the University’s future.
The resulting strategy is built around several key principles:
The value of higher education is clear and growing. Nebraska’s success is tied to that of its University.
Students come first. The University of Nebraska should be the best place in the country to be a student, providing high-quality, affordable, accessible education that prioritizes students’ mental and physical health and prepares them for post-graduation success.
Our people are our greatest asset. We will invest accordingly.
We have a responsibility to make the best use of every dollar Nebraskans entrust to us.
Themes of equity and inclusion touch everything we do. We will be a University for everyone—successful only when all voices are heard.
Finally, Nebraskans should know what to expect from their University. We must work every day to maintain the trust and confidence of the people of our state
The Vehicle, Spring 1985
Vol. 26, No. 2
Table of Contents
Beyond the FieldsKeila Tooleypage 3
Lonely Sculptor Accustomed to Living AloneMichelle Mitchellpage 4
Mona LisaBob Zordanipage 4
Poet Born in Pearl HarborAngelique Jenningspage 5
IntroductionsGraham Lewispage 6
Living InsideJennifer Soulepage 9
PictureKathy Greypage 10
Salvadore Dali in a Wheelchair on TVAngelique Jenningspage 11
Sonata in E FlatBecky Lawsonpage 12
Myopia and Wild KingdomMichelle Mitchellpage 12
On Becoming a GrandmotherKeila Tooleypage 13
A VisionJennifer D. Pringlepage 14
The Covered BridgeDebbie Woodleypage 14
Jacob\u27s LifeJoan Sebastianpage 15
ForgotGraham Lewispage 15
A Dozen and One TrainsongsAngelique Jenningspage 16
Women\u27s PlaceJennifer Soulepage 19
Night SailingKim Dumentatpage 20
She Isn\u27t There WhenMichelle Mitchellpage 20
A Case for the Common ColdMaggie Kennedypage 21
the cityTammy Batespage 22
The RattlesnakeEric S. McGeepage 22
New PictureKeila Tooleypage 23
Lewis and SinGraham Lewispage 24
Funny BarbecueBob Zordanipage 26
In a DreamF. Link Rapierpage 26
The Winter\u27s ColdJennifer Soulepage 27
Diary EntryTammy Batespage 27
Minor God and Patron Saint of Rabbits SpeaksAngelique Jenningspage 28
A MomentBrett Wilhelmpage 29
The Bishop SeatF. Link Rapierpage 30
The Thought of Being Rid of MyselfKeila Tooleypage 33
I Saw A ChildBea Cessnapage 33
Complacent gourmetGary Burrowspage 34
Night DreamsJennifer Soulepage 35
Changing ImagesAmy Callpage 35
Olsen Rug Co. Waterfall & ParkMaggie Kennedypage 36
Edge of the WildF. Link Rapierpage 37
DragonS. Hillpage 37
Harvests of CornBob Zordanipage 38
The Club JeromeGary Burrowspage 39
Tarzan And The CabPatrick Peterspage 39
The Rain That Never CameLynanne Feilenpage 40
Wonderment of the Far CrescentF. Link Rapierpage 40https://thekeep.eiu.edu/vehicle/1047/thumbnail.jp
The Vehicle, Fall 1985
Vol. 27, No. 1
Table of Contents
Satchel AssMichael D. Smithpage 3
PhotoDoug Andersonpage 7
CounselingChristy Dunphypage 8
Grave SiteJay D. Fiskpage 8
Sight-Seeing Outside PhoenixBob Zordanipage 9
PerformanceDan Von Holtenpage 10
NightmareKandy Bellpage 10
Photo (The Loft)Lawrence McGownpage 11
LaboringJanet Gracepage 12
Blood DonorDan Von Holtenpage 13
Photo (Pier)Lawrence McGownpage 14
ExamplesChristopher Albinpage 14
Three PoemsPatrick Peterspage 15
Sometimes I Dream in Cotton CandyKathy Graypage 16
One Day While BoatingF. Link Rapierpage 17
DepartureBob Zordanipage 17
140 Print That\u27s Life Peter Dowlingpage 18
Photo (Around the Bend)Mike Freckerpage 20
Light ConversationDan Hintzpage 22
She Waits For the WorldJim Harrispage 22
HoneyKathy Graypage 23
Photo AlbumPatrick Peterspage 24
Photo (Stairs)Lawrence McGownpage 25
Fallen From Grace to SaturdayF. Link Rapierpage 26
Post MortemF. Link Rapierpage 27
ConfessionJohn Kayserpage 27
Child\u27s PlayChristopher Albinpage 27
Seeking A Friend\u27s Advice on DietingKathy Graypage 28
PhotoDoug Andersonpage 28
She Came Back to MeJim Harrispage 29
Farm BoyDiana Winsonpage 30
DilemmaJanet Wilhelmpage 31
In a Rock or StoneRichard Donnellypage 32
In November He Came To MeJean Kover Chandlerpage 33
EndingChristy Dunphypage 34
The Honor GradEddie Simpsonpage 35
Photo (Thirst)Mike Freckerpage 40https://thekeep.eiu.edu/vehicle/1046/thumbnail.jp
A Multisite Preregistered Paradigmatic Test of the Ego-Depletion Effect
We conducted a preregistered multilaboratory project (k = 36; N = 3,531) to assess the size and robustness of ego-depletion effects using a novel replication method, termed the paradigmatic replication approach. Each laboratory implemented one of two procedures that was intended to manipulate self-control and tested performance on a subsequent measure of self-control. Confirmatory tests found a nonsignificant result (d = 0.06). Confirmatory Bayesian meta-analyses using an informed-prior hypothesis (δ = 0.30, SD = 0.15) found that the data were 4 times more likely under the null than the alternative hypothesis. Hence, preregistered analyses did not find evidence for a depletion effect. Exploratory analyses on the full sample (i.e., ignoring exclusion criteria) found a statistically significant effect (d = 0.08); Bayesian analyses showed that the data were about equally likely under the null and informed-prior hypotheses. Exploratory moderator tests suggested that the depletion effect was larger for participants who reported more fatigue but was not moderated by trait self-control, willpower beliefs, or action orientation.</p
- …