278 research outputs found
Frame-Independence of Exclusive Amplitudes in the Light-Front Quantization
While the particle-number-conserving convolution formalism established in the
Drell-Yan-West reference frame is frequently used to compute exclusive
amplitudes in the light-front quantization, this formalism is limited to only
those frames where the light-front helicities are not changed and the good
(plus) component of the current remains unmixed. For an explicit demonstration
of such criteria, we present the relations between the current matrix elements
in the two typical reference frames used for calculations of the exclusive
amplitudes, i.e. the Drell-Yan-West and Breit frames and investigate both
pseudoscalar and vector electromagnetic currents in detail. We find that the
light-front helicities are unchanged and the good component of the current does
not mix with the other components of the current under the transformation
between these two frames. Thus, the pseudoscalar and vector form factors
obtained by the diagonal convolution formalism in both frames must indeed be
identical. However, such coincidence between the Drell-Yan-West and Breit
frames does not hold in general. We give an explicit example in which the
light-front helicities are changed and the plus component of the current is
mixed with other components under the change of reference frame. In such a
case, the relationship between the frames should be carefully analyzed before
the established convolution formalism in the Drell-Yan-West frame is used.Comment: 14 pages, 4 figure
Maintenance of active chromatin states by HMGN2 is required for stem cell identity in a pluripotent stem cell model
Background:
Members of the HMGN protein family modulate chromatin structure and influence epigenetic modifications. HMGN1 and HMGN2 are highly expressed during early development and in the neural stem/progenitor cells of the developing and adult brain. Here, we investigate whether HMGN proteins contribute to the chromatin plasticity and epigenetic regulation that is essential for maintaining pluripotency in stem cells.
Results:
We show that loss of Hmgn1 or Hmgn2 in pluripotent embryonal carcinoma cells leads to increased levels of spontaneous neuronal differentiation. This is accompanied by the loss of pluripotency markers Nanog and Ssea1, and increased expression of the pro-neural transcription factors Neurog1 and Ascl1. Neural stem cells derived from these Hmgn-knockout lines also show increased spontaneous neuronal differentiation and Neurog1 expression. The loss of HMGN2 leads to a global reduction in H3K9 acetylation, and disrupts the profile of H3K4me3, H3K9ac, H3K27ac and H3K122ac at the Nanog and Oct4 loci. At endodermal/mesodermal genes, Hmgn2-knockout cells show a switch from a bivalent to a repressive chromatin configuration. However, at neuronal lineage genes whose expression is increased, no epigenetic changes are observed and their bivalent states are retained following the loss of HMGN2.
Conclusions:
We conclude that HMGN1 and HMGN2 maintain the identity of pluripotent embryonal carcinoma cells by optimising the pluripotency transcription factor network and protecting the cells from precocious differentiation. Our evidence suggests that HMGN2 regulates active and bivalent genes by promoting an epigenetic landscape of active histone modifications at promoters and enhancers
Poincare Invariant Algebra From Instant to Light-Front Quantization
We present the Poincare algebra interpolating between instant and light-front
time quantizations. The angular momentum operators satisfying SU(2) algebra are
constructed in an arbitrary interpolation angle and shown to be identical to
the ordinary angular momentum and Leutwyler-Stern angular momentum in the
instant and light-front quantization limits, respectively. The exchange of the
dynamical role between the transverse angular mometum and the boost operators
is manifest in our newly constructed algebra.Comment: 21 pages, 3 figures, 1 tabl
Long slit Spectropolarimetry of Jupiter and Saturn
We present ground-based limb polarization measurements of Jupiter and Saturn
consisting of full disk imaging polarimetry for the wavelength 7300 A and
spatially resolved (long slit) spectropolarimetry covering the wavelength range
5200 to 9350 A.
For the polar region of Jupiter we find for wl=6000 A a very strong radial
polarization with a seeing corrected maximum of about +11.5% in the South and
+10.0% in the North. Our model calculations demonstrate that the high limb
polarization can be explained by strongly polarizing (p~1.0), high albedo
(omega~0.98) haze particles with a scattering asymmetry parameter of g~0.6 as
expected for aggregate particles. The deduced particle parameters are
distinctively different when compared to lower latitude regions.
The spectropolarimetry of Jupiter shows a decrease in the polar limb
polarization towards longer wavelengths and a significantly enhanced
polarization in strong methane bands when compared to the adjacent continuum.
For lower latitudes the fractional polarization is small, negative, and it
depends only little on wavelength except for the strong CH4-band at 8870 A.
The South pole of Saturn shows a lower polarization (p~1.0-1.5%) than the
poles of Jupiter. The spectropolarimetric signal for Saturn decrease rapidly
with wavelength and shows no significant enhancements in the fractional
polarization in the absorption bands. These properties can be explained by a
vertically extended stratospheric haze region composed of small particles
<100nm.
In addition we find a previously not observed strong polarization feature
(p=1.5-2.0%) near the equator of Saturn. The origin of this polarization signal
is unclear but it could be related to a seasonal effect.Comment: Accepted for publication in ICARU
Selectivity and functional diversity in arbuscular mycorrhizas of co-occurring fungi and plants from a temperate deciduous woodland
1 The arbuscular mycorrhizal (AM) fungi colonizing plants at a woodland site in North Yorkshire (UK) have been characterized from the roots of five plant species (Rubus fruticosus agg. L., Epilobium angustifolium L., Acer pseudoplatanus L., Ajuga reptans L. and Glechoma hederacea L.), and identified using small-subunit rRNA (SSUrRNA) gene amplification and sequencing. 2 Interactions between five plant species from the site and four co-occurring glomalean fungi were investigated in artificial one-to-one AM symbioses. Three of the fungi were isolated from the site; the fourth was a culture genetically similar to a taxon found at the site. Phosphorus uptake and growth responses were compared with non-mycorrhizal controls. 3 Individual fungi colonized each plant with different spatial distribution and intensity. Some did not colonize at all, indicating incompatibility under the conditions used in the experiments. 4 Glomus hoi consistently occupied a large proportion of root systems and outperformed the other fungi, improving P uptake and enhancing the growth of four out of the five plant species. Only G. hoi colonized and increased P uptake in Acer pseudoplatanus, the host plant with which it associates almost exclusively under field conditions. Colonization of all plant species by Scutellospora dipurpurescens was sparse, and beneficial to only one of the host plants (Teucrium scorodonia). Archaeospora trappei and Glomus sp. UY1225 had variable effects on the host plants, conferring a range of P uptake and growth benefits on Lysimachia nummularia and T. scorodonia, increasing P uptake whilst not affecting biomass in Ajuga reptans and Glechoma hederacea, and failing to form mycorrhizas with A. pseudoplatanus. 5 These experimental mycorrhizas show that root colonization, symbiont compatibility and plant performance vary with each fungus-plant combination, even when the plants and fungi naturally co-exist. 6 We provide evidence of physical and functional selectivity in AM. The small number of described AM fungal species (154) has been ascribed to their supposed lack of host specificity, but if the selectivity we have observed is the general rule, then we may predict that many more, probably hard-to-culture glomalean species await discovery, or that members of species as currently perceived may be physiologically or functionally distinct
B_c meson rare decays in the light-cone quark model
We investigate the rare decays
and in the framework of the
light-cone quark model (LCQM). The transition form factors are calculated in
the space-like region and then analytically continued to the time-like region
via exponential parametrization. The branching ratios and longitudinal lepton
polarization asymmetries (LPAs) for the two decays are given and compared with
each other. The results are helpful to investigating the structure of
meson and to testing the unitarity of CKM quark mixing matrix. All these
results can be tested in the future experiments at the LHC.Comment: 9 pages, 11 figures, version accepted for publication in EPJ
Pion-photon and photon-pion transition form factors in light-cone formalism
We derive the minimal Fock-state expansions of the pion and the photon wave
functions in light-cone formalism, then we calculate the pion-photon and the
photon-pion transition form factors of and
processes by employing these
quark-antiquark wave functions of the pion and the photon. We find that our
calculation for the transition form factor
agrees with the experimental data at low and moderately high energy scale.
Moreover, the physical differences and inherent connections between the
transition form factors of and have been illustrated, which indicate that these
two physical processes are intrinsically related. In addition, we also discuss
the form factor and the decay width at .Comment: 20 pages, 2 figure
Exploring skewed parton distributions with two body models on the light front II: covariant Bethe-Salpeter approach
We explore skewed parton distributions for two-body, light-front wave
functions. In order to access all kinematical regimes, we adopt a covariant
Bethe-Salpeter approach, which makes use of the underlying equation of motion
(here the Weinberg equation) and its Green's function. Such an approach allows
for the consistent treatment of the non-wave function vertex (but rules out the
case of phenomenological wave functions derived from ad hoc potentials). Our
investigation centers around checking internal consistency by demonstrating
time-reversal invariance and continuity between valence and non-valence
regimes. We derive our expressions by assuming the effective qq potential is
independent of the mass squared, and verify the sum rule in a non-relativistic
approximation in which the potential is energy independent. We consider
bare-coupling as well as interacting skewed parton distributions and develop
approximations for the Green's function which preserve the general properties
of these distributions. Lastly we apply our approach to time-like form factors
and find similar expressions for the related generalized distribution
amplitudes.Comment: 25 pages, 12 figures, revised (minor changes but essential to
consistency
Transient increase in atherosclerotic plaque macrophage content following Streptococcus pneumoniae pneumonia in ApoE-deficient mice
Introduction: Despite epidemiological associations between community acquired pneumonia (CAP) and myocardial infarction, mechanisms that modify cardiovascular disease during CAP are not well defined. In particular, largely due to a lack of relevant experimental models, the effect of pneumonia on atherosclerotic plaques is unclear. We describe the development of a murine model of the commonest cause of CAP, Streptococcus pneumoniae pneumonia, on a background of established atherosclerosis. We go on to use our model to investigate the effects of pneumococcal pneumonia on atherosclerosis.
Methods: C57BL/6J and ApoE-/- mice were fed a high fat diet to promote atherosclerotic plaque formation. Mice were then infected with a range of S. pneumoniae serotypes (1, 4 or 14) with the aim of establishing a model to study atherosclerotic plaque evolution after pneumonia and bacteremia. Laser capture microdissection of plaque macrophages enabled transcriptomic analysis.
Results: Intratracheal instillation of S. pneumoniae in mice fed a cholate containing diet resulted in low survival rates following infection, suggestive of increased susceptibility to severe infection. Optimization steps resulted in a final model of male ApoE-/- mice fed a Western diet then infected by intranasal instillation of serotype 4 (TIGR4) S. pneumoniae followed by antibiotic administration. This protocol resulted in high rates of bacteremia (88.9%) and survival (88.5%). Pneumonia resulted in increased aortic sinus plaque macrophage content 2 weeks post pneumonia but not at 8 weeks, and no difference in plaque burden or other plaque vulnerability markers were found at either time point. Microarray and qPCR analysis of plaque macrophages identified downregulation of two E3 ubiquitin ligases, Huwe1 and Itch, following pneumonia. Treatment with atorvastatin failed to alter plaque macrophage content or other plaque features.
Discussion: Without antibiotics, ApoE-/- mice fed a high fat diet were highly susceptible to mortality following S. pneumoniae infection. The major infection associated change in plaque morphology was an early increase in plaque macrophages. Our results also hint at a role for the ubiquitin proteasome system in the response to pneumococcal infection in the plaque microenvironment
Interactions between arbuscular mycorrhizal fungi and intraspecific competition affect size and size inequality of Plantago lanceolata L.
Intraspecific competition causes decreases in plant size and increases in size inequality. Arbuscular mycorrhizas usually increase the size and inequality of non-competing plants, but mycorrhizal effects often disappear when plants begin competing. We hypothesized that mycorrhizal effects on size inequality would be determined by the experimental conditions, and conducted simultaneous field and glasshouse experiments to investigate how AM fungi and intraspecific competition determine size inequality in Plantago lanceolata.
2 As predicted, plant size was reduced when plants were competing, in both field and controlled conditions. However, size inequality was unexpectedly reduced by competition. Plants may have competed in a symmetric fashion, probably for nutrients, rather than the more common situation, in which plant competition is strongly asymmetric.
3 Mycorrhizas had no effect on plant size or size inequality in competing plants in either field or controlled conditions, possibly because competition for nutrients was intense and negated any benefit the fungi could provide.
4 The effects of mycorrhizas on non-competing plants were also unexpected. In field-grown plants, AM fungi increased plant size, but decreased size inequality: mycorrhizal plants were more even in size, with few very small individuals. In glasshouse conditions, mycorrhizal colonization was extremely high, and was generally antagonistic, causing a reduction in plant size. Here, however, mycorrhizas caused an increase in size inequality, supporting our original hypothesis. This was because most plants were heavily colonized and small, but a few had low levels of colonization and grew relatively large.
5 This study has important implications for understanding the forces that structure plant communities. AM fungi can have a variety of effects on size inequality and thus potentially important influences on long-term plant population dynamics, by affecting the genetic contribution of individuals to the next generation. However, these effects differ, depending on whether plants are competing or not, the degree of mycorrhizal colonization and the responsiveness of the plant to different colonization densities
- …