Mémoires de l'Académie des sciences, inscriptions et belles-lettres de Toulouse

19711971 (VOL133,SER15,T2)-1971.Appartient à l’ensemble documentaire : MidiPyren

Microsatellite data for Gobionotothen gibberifrons

Data for 9 microsatellite loci for 165 Gobionotothen gibberifrons specimens from six sampling locations (Elephant Island, King George Island, Joinville Island a&b, South Georgia, South Sandwich Islands). The file is in MSA format (MSA=Microsatellite Analyzer: http://i122server.vu-wien.ac.at/MSA/MSA_download.html). Raw microsatellite data was transformed using a beta version of the software tandem (http://evolution.unibas.ch/salzburger/software/tandem.htm)

Experimental set-up (schematic view).

<p>(A) The two-way choice set-up showing the egg-traps and the shelters, which are permeable to eggs. (B) The four-way choice set-up (‘partial partition’ method) with semi-permeable grids, passable for females but not for males. (C) The set-up for the male aggression trials with stimulus males in plastic cylinders and the focal male hiding in the shelter.</p

Additional file 1: Table S1. of Parental investment matters for maternal and offspring immune defense in the mouthbrooding cichlid Astatotilapia burtoni

List of all primers used for candidate gene expression: Table depicts gene names, functions, fwd and rev sequences and references for those, which have not been designed by the authors. (PDF 43 kb

The egg-spots of haplochromine cichlids as exemplified in <i>Astatotilapia burtoni</i>.

<p>(A) A male of <i>A. burtoni</i> showing egg-spots on its anal fin. (B) Natural variation of egg-spots in <i>A. burtoni</i>. All these fish were caught and photographed at the south-eastern part of Lake Tanganyika in Zambia. (C) A typical courtship and mating cycle of a haplochromine starts with a lateral display of the male, to which the female responds; she then lays a clutch of eggs and immediately takes them up into her mouth. The male then presents the egg-spots on the anal fin; the female seemingly nuzzles at these egg-spots and the male releases sperm so that the eggs are fertilized within the females' mouth. The eggs and larvae then stay in the buccal cavity of the female for a period of several days to a few weeks. The arrow points to the location of an egg that the female is taking up into her mouth.</p

Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes-4

<p><b>Copyright information:</b></p><p>Taken from "Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes"</p><p>http://www.biomedcentral.com/1741-7007/5/51</p><p>BMC Biology 2007;5():51-51.</p><p>Published online 15 Nov 2007</p><p>PMCID:PMC2254590.</p><p></p>(c) as well as in the growing egg-dummies (d; the position of the yellowish inner circle, , and the transparent outer ring, , is indicated). Note that the yellow pigments of the egg-spots are removed during tissue processing. (e-h) In male sp. 'bicolor' (e), for which we used a morph with only one egg-spot (f), expression was detected throughout the entire egg-spot (g), and it appears that the dark melanophores arrange around the center of expression (h), just as reported for zebrafish, where is indirectly also responsible for melanophore organization [25]. (i-l) In male (i), is expressed in the yellow blotches of the anal fin (j, k), and also in the pearly spots ('') on the posterior part of the anal fin (l). (m-p) In the basal riverine haplochromine (m), the male anal fins do not exhibit egg-spot like blotches (n) and no expression could be detected either (o, p). (q-u) is expressed in the yellowish/orange areas of the pearly spot pattern on the dorsal fins of male (q-s) and (t, u). (v-z) is expressed in the egg-dummies on the tassels at the tips of the conspicuously elongated pelvic fins of the ectodine cichlid (v-x). Female fins of (y) do not show expression (z). The control experiments show that is not expressed in female anal fins or in the "sense" control. Arrowheads refer to identical points in sequential images

Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes-3

<p><b>Copyright information:</b></p><p>Taken from "Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes"</p><p>http://www.biomedcentral.com/1741-7007/5/51</p><p>BMC Biology 2007;5():51-51.</p><p>Published online 15 Nov 2007</p><p>PMCID:PMC2254590.</p><p></p>consists of xanthophores, the pteridine pigments of which fluoresce at high pH. Melanophores are also present in the border area around the transparent outer ring (a melanophore is marked by an arrowhead). (d, e) Egg-spot of ; bright field (d) and fluorescence image (e)

Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes-2

<p><b>Copyright information:</b></p><p>Taken from "Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes"</p><p>http://www.biomedcentral.com/1741-7007/5/51</p><p>BMC Biology 2007;5():51-51.</p><p>Published online 15 Nov 2007</p><p>PMCID:PMC2254590.</p><p></p>y, were not sequenced). (b) N/S ratio of haplochromines compared to non-haplochromine cichlids as revealed from a sliding window analysis with DNASP. (c) Schematic representation of the structure of the Csf1ra protein. The gene consists of an extracellular ligand-binding domain containing five immunoglobulin-like (Ig-like) domains with conserved cysteines (CYS), a transmembrane domain (TMD), and an intracellular partition that contains two tyrosine kinase (TK) domains interrupted by a kinase insert domain. In the tyrosine kinase I domain (TK I) a glycine-rich region (GLY-rich) and a conserved leucine (LEU) is found, the tyrosine kinase II domain (TK II) contains a conserved tyrosine (TYR). The asterisk indicates that in one species, , the otherwise conserved cysteine at amino acid position 450 has been replaced by a tryptophan. (d) Maximum likelihood phylogeny based on more than 4100 bp of non-coding sequences of the gene locus corroborating the monophyly of the haplochromines (yellow box) and the ancestral position of , , and [7]. The asterisk indicates relatively short branches not supported by high bootstrap values or a Shimodaira-Hasegawa test. (e) Branch-scaled tree showing the N/S rates reconstructed with HyPhy. The only internal branch with a N/S > 1 is the one representing the common ancestor of the haplochromines (yellow box)

Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes-0

<p><b>Copyright information:</b></p><p>Taken from "Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes"</p><p>http://www.biomedcentral.com/1741-7007/5/51</p><p>BMC Biology 2007;5():51-51.</p><p>Published online 15 Nov 2007</p><p>PMCID:PMC2254590.</p><p></p>consists of xanthophores, the pteridine pigments of which fluoresce at high pH. Melanophores are also present in the border area around the transparent outer ring (a melanophore is marked by an arrowhead). (d, e) Egg-spot of ; bright field (d) and fluorescence image (e)

Meyer et al., supplementary information

Meyer et al., supplementary informatio