THE ASSOCIATION OF GENE POLYMORPHISMS WITH ATHLETE STATUS IN UKRAINIANS

Athletic performance is a polygenic trait influenced by both environmental and genetic factors. Objective: to investigate individually and in combination the association of common gene polymorphisms with athlete status in Ukrainians. Methods: A total of 210 elite Ukrainian athletes (100 endurance-oriented and 110 power-orientated athletes) and 326 controls were genotyped for ACE I/D, HIF1A Pro582Ser, NOS3 –786 T/C, PPARA intron 7 G/C, PPARG Pro12Ala and PPARGC1B Ala203Pro gene polymorphisms, most of which were previously reported to be associated with athlete status or related intermediate phenotypes in different populations. Results: Power-oriented athletes exhibited an increased frequency of the HIF1A Ser (16.1 vs. 9.420P = 0.034) and NOS3 T alleles (78.3 vs. 66.220P = 0.0019) in comparison with controls. Additionally, we found that the frequency of the PPARG Ala allele was significantly higher in power-oriented athletes compared with the endurance-oriented athletes (24.7 vs. 13.520P = 0.0076). Next, we determined the total genotype score (TGS, from the accumulated combination of the three polymorphisms, with a maximum value of 100 for the theoretically optimal polygenic score) in athletes and controls. The mean TGS was significantly higher in power-oriented athletes (39.1 ± 2.3 vs. 32.6 ± 1.5; P = 0.0142) than in controls. Conclusions: We found that the HIF1A Ser, NOS3 T and PPARG Ala alleles were associated with power athlete status in Ukrainians

Dependence of Aerobic Performance of Athletes on Polymorphism of Genes

The adaptation of an athlete to systematic physical exercise has been shown to be determined by a combination of great many genes. The aim of our study was to investigate the dependence of the aerobic capacity parameters in sport on the set of gene polymorphisms. Cardio-respiratory system (CRS) adaptation reactions to exercise of 72 endurance athletes were assessed using the gas analysis. The analysis of the obtained results has shown both single and combined effect of the gene polymorphisms on the aerobic capacity. The impact of 6 polymorphisms on the aerobic performance level was analyzed: Т–786→С polymorphism of the promoter of еNOS gene as well as АСЕ I/D polymorphism, Рго/Ala polymorphism of PPARG gene, G/C polymorphism of PPARA gene, Pro582Ser polymorphism of HIF1α gene, and Ala203Pro polymorphism of PPARGC1B. It was found that a single impact on the HRmax providing АСЕ I/D polymorphism. Individual influence of АСЕ gene accounts for 2% of this index dissipation. Results showed that there is a dependence between the amount the maximum volume of consumed oxygen (VO2max) from the set of gene polymorphisms. Cumulative impact of these polymorphisms in the combination with the individual parameters (gender; qualification; kind of sport) stipulates 71% of dispersion of VO2max value

Результаты контроля доз облучения хрусталиков глаз у медицинского персонала г. Санкт-Петербурга

Results of individual monitoring for personnel of X-ray surgical teams in several clinics of St. Petersburg are presented and analyzed. Measurements of the operational quantities – individual dose equivalents Hp (3) and Hp (10) were performed by thermoluminescent dosimetry method. Dosimeters designed to measure Hp (3) were located in the operators forehead area, and to determine Hp (10) both above the operator ‘s individual protective apron in the collar or chest area and under the protective apron in the chest area. The results of 34 measurements of the annual values of Hp (3) and Hp (10) measured above the apron and 24 values of Hp (10) measured below the apron were processed and analyzed. The results after the statistical treatment show that the probability of exceeding the annual values of Hp (3) in the personnel of X-ray surgical teams of the new dose limit 20 mSv is small, less than 1%. Exceeding the current dose limit of the equivalent exposure dose of the lens of the eye (150 mSv) is hardly possible at all under normal conditions. The best solution for evaluating the radiation dose of the lens of the eye is to measure the individual equivalent of the dose Hp (3), using a suitably calibrated TL-dosimeter (thermoluminescent dosimeter) located near the worker’s eyes. However, this additional dosimeter is only necessary when the values of eye lens equivalent dose can approach the new value of dose limit of 20 mSv. According to the results of the study, it is possible to introduce such an additional dosimeter if the annual value of Hp (10) recorded by the dosimeter located above the protective apron is more than 10 mSv.В работе представлены и проанализированы результаты индивидуального дозиметрического контроля персонала рентгенохирургических бригад нескольких клинических больниц г. Санкт-Петербурга. Измерения операционных величин – индивидуальных эквивалентов доз Нр (3) и Нр (10) проводились методом термолюминесцентной дозиметрии. Дозиметры, предназначенные для измерения Нр (3), располагались в области лба оператора, а для определения Нр (10) – как над индивидуальным защитным фартуком оператора в области воротничка или груди, так и под защитным фартуком в области груди. Были обработаны и проанализированы результаты 34 измерений годовых значений Нр (3) и Нр (10), измеренных над фартуком, и 24 значений Нр (10), измеренных под фартуком. Результаты статистической обработки показали, что вероятность превышения годовых значений Нр (3) у персонала рентгенохирургических бригад нового предела дозы 20 мЗв мала, – менее 1%. Превышение же существующего в настоящее время дозового предела эквивалентной дозы облучения хрусталика глаза (150 мЗв) вообще вряд ли возможно в нормальных условиях. Наилучшим решением для оценки дозы облучения хрусталика глаза является измерение индивидуального эквивалента дозы Нр (3) с помощью соответствующим образом откалиброванного ТЛ-дозиметра (термолюминесцентный дозиметр), расположенного вблизи глаз работника. Однако этот дополнительный дозиметр необходим только в случае, когда значение эквивалентной дозы облучения хрусталика глаза может приблизиться к новому значению предела дозы 20 мЗв. По результатам проведенного исследования введение такого дополнительного дозиметра возможно, если годовое значение Нр (10), регистрируемое дозиметром, расположенным над защитным фартуком, больше 10 мЗв

Translational Database Selection and Multiplexed Sequence Capture for Up Front Filtering of Reliable Breast Cancer Biomarker Candidates

Biomarker identification is of utmost importance for the development of novel diagnostics and therapeutics. Here we make use of a translational database selection strategy, utilizing data from the Human Protein Atlas (HPA) on differentially expressed protein patterns in healthy and breast cancer tissues as a means to filter out potential biomarkers for underlying genetic causatives of the disease. DNA was isolated from ten breast cancer biopsies, and the protein coding and flanking non-coding genomic regions corresponding to the selected proteins were extracted in a multiplexed format from the samples using a single DNA sequence capture array. Deep sequencing revealed an even enrichment of the multiplexed samples and a great variation of genetic alterations in the tumors of the sampled individuals. Benefiting from the upstream filtering method, the final set of biomarker candidates could be completely verified through bidirectional Sanger sequencing, revealing a 40 percent false positive rate despite high read coverage. Of the variants encountered in translated regions, nine novel non-synonymous variations were identified and verified, two of which were present in more than one of the ten tumor samples

Visual Parameterization of Vaganov-Shashkin Simulation Model and its Application in Dendroecological Research

В дендрохронологии используется большое количество различных аналитических подходов для анализа данных, одним из которых является моделирование. При создании любой модели одним из главных вопросов выступает выбор главных факторов. Самые распространенные и доступные климатические данные – температура и осадки. На основании имитационной модели Ваганова-Шашкина предложен новый алгоритм визуальной параметризации роста годичных колец хвойных деревьев, названный «VS- осциллограф», и описана его программная реализация. Полученный алгоритм был апробирован на двух породах древесных растений – Larix gmelini и Picea obovata. Новый способ параметризации и анализ моделируемых результатов позволяют оценить локальные условия произрастания древесных растений на основе динамики двух климатических переменных: температуры и осадков, без привлечения дополнительной информации о местообитанииThere are many different methods and tools for data analysis in dendrochronology. Modeling is one of them. One of the main issues in modeling is a choice of the main factors. Сlimatic data (temperature and precipitation) are the most common and affordable of them. Based on Vaganov – Shaskin model the new algorithm of visual parameterization of three-ring growth – VS-oscilloscope was developed. Algorithm was tested on different species of woody plants – Larix gmelini and Picea obovata. A new parameterization and analysis of modeling results help to evaluate conditions of area of growth of woody plants, based on dynamic of two climate variables: temperature and precipitation, without adding information about area of growt

Results of eye lens doses control of medical personnel in St. Petersburg

Results of individual monitoring for personnel of X-ray surgical teams in several clinics of St. Petersburg are presented and analyzed. Measurements of the operational quantities – individual dose equivalents Hp (3) and Hp (10) were performed by thermoluminescent dosimetry method. Dosimeters designed to measure Hp (3) were located in the operators forehead area, and to determine Hp (10) both above the operator ‘s individual protective apron in the collar or chest area and under the protective apron in the chest area. The results of 34 measurements of the annual values of Hp (3) and Hp (10) measured above the apron and 24 values of Hp (10) measured below the apron were processed and analyzed. The results after the statistical treatment show that the probability of exceeding the annual values of Hp (3) in the personnel of X-ray surgical teams of the new dose limit 20 mSv is small, less than 1%. Exceeding the current dose limit of the equivalent exposure dose of the lens of the eye (150 mSv) is hardly possible at all under normal conditions. The best solution for evaluating the radiation dose of the lens of the eye is to measure the individual equivalent of the dose Hp (3), using a suitably calibrated TL-dosimeter (thermoluminescent dosimeter) located near the worker’s eyes. However, this additional dosimeter is only necessary when the values of eye lens equivalent dose can approach the new value of dose limit of 20 mSv. According to the results of the study, it is possible to introduce such an additional dosimeter if the annual value of Hp (10) recorded by the dosimeter located above the protective apron is more than 10 mSv

Visual Parameterization of Vaganov-Shashkin Simulation Model and its Application in Dendroecological Research

В дендрохронологии используется большое количество различных аналитических подходов для анализа данных, одним из которых является моделирование. При создании любой модели одним из главных вопросов выступает выбор главных факторов. Самые распространенные и доступные климатические данные – температура и осадки. На основании имитационной модели Ваганова-Шашкина предложен новый алгоритм визуальной параметризации роста годичных колец хвойных деревьев, названный «VS- осциллограф», и описана его программная реализация. Полученный алгоритм был апробирован на двух породах древесных растений – Larix gmelini и Picea obovata. Новый способ параметризации и анализ моделируемых результатов позволяют оценить локальные условия произрастания древесных растений на основе динамики двух климатических переменных: температуры и осадков, без привлечения дополнительной информации о местообитанииThere are many different methods and tools for data analysis in dendrochronology. Modeling is one of them. One of the main issues in modeling is a choice of the main factors. Сlimatic data (temperature and precipitation) are the most common and affordable of them. Based on Vaganov – Shaskin model the new algorithm of visual parameterization of three-ring growth – VS-oscilloscope was developed. Algorithm was tested on different species of woody plants – Larix gmelini and Picea obovata. A new parameterization and analysis of modeling results help to evaluate conditions of area of growth of woody plants, based on dynamic of two climate variables: temperature and precipitation, without adding information about area of growt

Evolution and Interdependence of Structure and Properties of Nanocomposites of Multiwall Carbon Nanotubes with Polyaniline

Structure–property relationships of nanocomposites of multiwall carbon nanotubes (MWCNTs) and polyaniline (PANI) doped with dodecylbenzenesulfonic acid (DBSA) are studied and discussed in terms of contribution of the PANI phase properties. Two phases of PANI-DBSA with different properties, such as a shell-wrapping carbon nanotube and separate neat PANI-DBSA domains, are revealed for the first time in the nanocomposites with a high or medium PANI-DBSA content. In the nanocomposites with lower PANI-DBSA contents (<40 wt %), only a core–shell morphology with ∼3 nm or lower thickness PANI shell on the nanotubes is observed. Under this condition, the degree of crystallinity of the PANI-DBSA shell increases almost 2-fold, and thermal stability of the nanocomposite grows sharply. As compared with the neat PANI-DBSA, this special PANI shell phase has more perfect molecular structure and is characterized by a reduced amount of DBSA. It is found that the latter is caused by a competition between the processes of doping of the formed PANI in the shell by the protonic acid and p-doping caused by the MWCNT surface. The nanocomposites demonstrate ppm/ppb range sensitivity to ammonia/methylamine gases with response times, which inversely depend on the PANI-DBSA contents and thickness of the shell