Efficient Database Evolution in Digital Library Reengineering

Cursos e Congresos, C-155[Abstract] With the advancement of internet applications, extensive information systems were created to effectively manage and provide easy access to documents, which coincided with a global initiative to convert physical documents into digital format, making them accessible through the internet. After two decades, these databases are well-structured and organized, although the software used to manage them is gradually becoming outdated. Additionally, once the initial digitization and creation of metadata are completed, it is sensible to enhance the metadata further to provide more detailed information about the documents. In this article we propose a tool to facilitate the evolution of large documentary databasesXunta de Galicia; ED431C 2021/53CITIC is funded by the Xunta de Galicia through the collaboration agreement between the Consellería de Cultura, Educación, Formación Profesional e Universidades and the Galician universities for the reinforcement of the research centres of the Galician University System (CIGUS), and by PRE2021-099351, MCIN/AEI+”FSE+”;GRC[ED431C 2021/53]: GAIN/Xunta de Galicia; TED2021-129245B-C21(PLAGEMIS): MCIN/AEI+”NextGenerationEU“/PRTR; PID2020-114635RB-I00(EXTRACompact): MCIN/AEI; PID2021-122554OB-C33 (OASSIS): MCIN/AEI+EU/ERDF A way of making Europe; PDC2021-120917-C21 (SIGTRANS): MCIN/AEI+“NextGenerationEU”/PRT

Comparison of temporary or continuous immersion systems for micropropagation in liquid medium of axillary shoots of Salix viminalis.

In this study the response to culture in liquid medium of axillary shoots of two woody species, chestnut and willow, has been compared. Both species differ in their recalcitrance to micropropagation, especially in their rooting and acclimatization abilities. Parameters such as explant type, supporting material, bioreactor type, number of immersions and/or aerations were tested. Continuous and temporary immersion systems have been applied to both species and several differences were detected. Hyperhydricity was not observed in willow shoots cultured in liquid medium with aeration, but this disorder affected chestnut cultures unless supporting materials as rockwool cubes were used. Besides, willow shoots rooted spontaneously in liquid culture, whereas chestnut shoots required an auxin treatment. In spite of these differences, both species showed better performance in liquid culture than in semi-solid medium, and shoots from an adult willow genotype and from eight selected chestnut genotypes (hybrids of European and Asian chestnuts) were successfully rooted and acclimatized.Peer reviewe

Effect of NPA on adventitious root induction and root development in leaves of chestnut microshoots

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Caracterización y análisis de expresión del gen TCTP en fagáceas

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Micropropagación de Salix viminalis en medio líquido

El Salix viminalis o mimbre blanco es un arbusto o árbol de pequeño tamaño que suele crecer cerca de corrientes de agua y lugares húmedos. Utilizado en cestería, en la actualidad es un candidato para la producción de bioenergía y programas de fitorremediación. En este trabajo se ha investigado su micropropagación en medio líquido, tanto en sistemas de inmersión temporal como de inmersión continua, así como su capacidad de crecimiento en condiciones fotoautotróficasPeer reviewe

The Effect of Sucrose Supplementation on the Micropropagation of Salix viminalis L. Shoots in Semisolid Medium and Temporary Immersion Bioreactors

[Abstract] The effect of sucrose concentration on the micropropagation of axillary shoots of willow was investigated. The following factors were examined: the culture system (semisolid medium in glass jars versus liquid medium in temporary immersion bioreactors), the type of explant (apical and basal sections), the frequency of immersion, and CO₂ enrichment. Shoots and leaf growth were significantly higher in RITA® bioreactors than in the jars for all the sucrose treatments. Apical or basal sections of willow cultured in bioreactors under high light intensity (150 µmol m⁻² s⁻¹) and ventilated six times a day with CO₂-enriched air were successfully proliferated without sucrose, whereas shoots cultured in jars did not proliferate well if sucrose concentration was 0.5% or lower. More roots were formed when sucrose was added to the medium. Shoots cultured in bioreactors were successfully acclimatized irrespective of the sucrose treatment and the root biomass when transferred to ex vitro conditions. This is the first report of photoautotrophic willow micropropagation, our results confirm the importance of proper gaseous exchange to attain autotrophy during in vitro propagation.This research was funded by Xunta de Galicia (Spain) (projects IN607A and Contrato Programa 2019-2020), by CYTED (P117RT0522) and by CSIC (COOPB20584)Xunta de Galicia; IN607AConsejo Superior de Investigaciones Científicas; COOPB2058

Evaluación de forjados de madera reforzados con recrecidos de hormigones ligeros estructurales

El refuerzo de forjados existentes de madera incrementando su capacidad resistente y/o su rigidez es una intervención estructural muy extendida. El refuerzo mediante recrecidos de hormigón unidos solidariamente a las viguetas de madera mediante conexiones metálicas presenta las siguientes ventajas: Incremento de la resistencia y rigidez del forjado inicial; el forjado se transforma en un diafragma rígido frente a las acciones horizontales y, cuando es posible ejecutar el refuerzo sin necesidad de apuntalar, puede mantenerse el uso de la zona inferior al forjado que se está reforzando. El principal inconveniente de la técnica radica en la carga añadida por el peso del hormigón. El empleo de hormigones ligeros estructurales (HLE) constituye una alternativa atractiva frente a los hormigones convencionales (HC), al aunar todas las ventajas de la técnica y disminuir considerablemente los inconvenientes debido al menor peso del hormigón ligero. Se han comparado los resultados obtenidos en la evaluación de forjados de madera de distintas luces, secciones y sobrecargas, reforzados con recrecidos de HC y de HLE. En concreto se han estudiado 3 luces (4.0, 4.5 y 5.0m) y 2 sobrecargas de uso (2kN/m2 y 3kN/m2). En todos los casos la sección de la madera se ha obtenido con criterios antiguos de dimensionamiento, utilizando las luces y acciones de los forjados a evaluar

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

[Abstract] In this study, we propagated two old Galician plum varieties in liquid medium using a temporary immersion system with RITA© bioreactors. Environmental variables including culture system, light intensity, CO2 enrichment, immersion frequency and sucrose supplementation were evaluated in relation to in vitro proliferation, physiological status and ex vitro performance. Bioreactors were superior to jars for culturing shoots in photomixotrophic conditions, producing up to 2 times more shoot numbers and up to 1.7 times more shoot length (depending on the genotype) using shoot clusters. The number and quality of shoots were positively influenced by the sucrose concentration in the medium, plus by the light and gaseous environment. For individual apical sections the best response occurred with 3% sucrose, 150 µmol m−2 s−1 photosynthetic photon flux density and 2000 ppm CO2, averaging 2.5 shoots per explant, 26 mm shoot length and 240 mm2 leaf area, while with 50 µmol m−2 s−1 light and ambient CO2 (400 ppm) values decreased to 1.2 shoots per explant, 14 mm of shoot length and 160 mm2 of leaf area. Shoots cultured photoautotrophically (without sucrose) were successfully rooted and acclimated despite of showing limited growth, low photosynthetic pigments, carbohydrate, phenolic and antioxidant contents during the multiplication phase.This research was funded by Xunta de Galicia (Spain) (project IN607A 2021), by CYTED (P117RT0522), and by CSIC (PIE 202140E015, COOPB20584)Xunta de Galicia; IN607A 2021Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED); P117RT0522Consejo Superior de Investigaciones Científicas; COOPB2058

Analysis of the hormonal manipulation during in vitro induction of adventitious roots in Castanea Sativa

Adventitious rooting is a complex process still poorly understood, and is a crucial step for the clonal propagation of elite genotypes of woody species. The aim of this work was to develop a new, simple and reproducible experimental system in chestnut to study the adventitious rooting process at the physiological and molecular levels. Chestnut microshoots and leaf explants excised from microshoots were used to evaluate their physiological responses to indole-3-butyric acid alone or in combination with N-1-naphthyl-phthalamic acid. Similar rooting percentages were obtained in auxin treated microshoots and leaves, however, the mean number of roots per rooted explant was higher in microshoots than in leaves. When NPA was included in the medium, the rooting capacity of both, leaves and microshoots, was reduced and root emergence was delayed at least 4 days compared with explants treated with IBA alone. Whereas in microshoots less than half of the roots were developed from the basal callus, in leaf explants the majority of the roots were originated from the callus developed in the leaf petiole, which made this system very useful for the identification of auxin-responsive genes and key regulatory genes involved in the early events of adventitious rooting process.Peer reviewe

Effect of Sucrose on Growth and Stress Status of Castanea sativa x C. crenata Shoots Cultured in Liquid Medium

[Abstract] Current breeding programs aim to increase the number of ink-tolerant chestnut trees using vegetative propagation of selected genotypes. However, the commercial vegetative propagation of chestnut species is still a bottleneck for the forest industry, mainly due to problems in the rooting and acclimation of propagules. This study aimed to explore the potential benefits of decreasing sucrose supplementation during chestnut micropropagation. Explants were cultured with high light intensity and CO2-enriched air in temporary or continuous immersion bioreactors and with different sucrose supplementation to evaluate the impact of these treatments on growth, rooting and physiological status (monosaccharide content, soluble phenolics and antioxidant activity). The proliferation and rooting performance of shoots cultured by continuous immersion decreased sharply with sucrose concentrations lower than 1%, whereas shoots cultured by temporary immersion grew and rooted successfully with 0.5% sucrose. These results suggest this system is appropriate to culture chestnut with low sucrose concentration and to explore photoautotrophic propagation of this species.This research was funded by Xunta de Galicia (Spain) (projects IN607A 2021), by CYTED (P117RT0522), and by CSIC (PIE 202140E015, COOPB20584)Xunta de Galicia; IN607A 2021Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED); P117RT0522Consejo Superior de Investigaciones Científicas; PIE 202140E015Consejo Superior de Investigaciones Científicas; COOPB2058