EXPERIMENTAL AND COMPUTATIONAL STUDY OF BINDING INTERACTION OF ALKOXY DERIVATIVES OF N-ARYLHYDROXAMIC ACIDS WITH DNA

Objective: Binding affinity towards DNA for small molecules is very important in the development of new therapeutic reagents. Interaction between N-Arylhydroxamic acid derivatives N-p-Tolyl-4-Ethoxybenzohydroxamic acid (p-TEBHA) and N-o- Tolyl-4-Ethoxybenzohydroxamic Acid (o-TEBHA), with calf thymus DNA (CT DNA) were studied by UV–visible absorption, fluorescence,  viscosity measurement and molecular docking. Methods: The absorption and emission spectra of DMSO solution of hydroxamic acid derivatives were studied for their binding activity with calf-thymus DNA by titration with increasing amount hydroxamic acids. Docking was performed by HEX software. Results: Fluorescent spectra showed that CT DNA quenches the emission spectra of p-TEBHA & o-TEBHA with binding constant 1.6 Ñ… 108 M-1 and 4.3 Ñ… 103 M-1 respectively. Competitive study with ethidium bromide (EB) indicates that p-TEBHA can displace the DNA-bound EB suggesting strong competition with EB whereas o-TEBHA does not displace the DNA-bounded Ethidium bromide effectively. UV study of the interaction of the complexes with calf-thymus DNA has shown that the hydroxamic acid derivatives can bind with CT DNA. The docking studies were used to predict the mode of interaction of the drug with DNA. It was observed that as far as binding strength was concerned the computational results complemented the experimental results. Conclusion: Investigated hydroxamic acid derivatives are found to be strong DNA binders and seem to have promising drug like nature

Role of ferric carboxymaltose in battle with anemia among north Indian pregnant women

Background: Anemia among pregnant women is a serious global health concern. Anemia is a major hematological, nutritional deficiency but still a manageable health problem among the pregnant women and very common in developing countries like India in under privileged population. Increased morbidity and mortality is seen in pregnant women and their foetuses suffering from anemia. Aim of this study was to study safety and efficacy of injection ferric carboxymaltose in pregnant women with iron deficiency anemia. Methods: This is a Prospective interventional comparative randomized study carried among antenatal women in the Department of Obstetrics and Gynaecology, S. N. Medical College, Agra from (May 2021-October 2022), comprised of 200 pregnant women divided into two groups. Group A-100 antenatal women were transfused with iron sucrose (IS), Group B-100 antenatal women were transfused with ferric carboxymaltose (FCM). Results: In FCM group after 3 weeks of post transfusion hemoglobin level was 10.5±0.44 and in iron sucrose group it was 9.9±0.59 (p=0.0001). 6 week post transfusion hemoglobin level in FCM group was 11.37±0.62 and in iron sucrose group it was 10.45±0.59 (p=0.0001). Average rise in hemoglobin in FCM group after 3 week post transfusion was 2.9±0.02 and after 6 week it was 3.77±0.16. In iron sucrose group at 3 week it was 2.1±0.06 and at 6 week it was 2.65±0.04. The average rise in hemoglobin level was greater among patients of FCM compared to Iron sucrose group. Conclusions: Ferric carboxymaltose was safe and more effective in treatment of iron deficiency anaemia in pregnant women as compared to iron sucrose with lesser side effect and better patient compliance

Comorbidities in paediatric psoriasis: Experience from a tertiary care centre

Background: Psoriasis begins in childhood in around one-third of the cases. There has been conflicting evidence regarding the association of paediatric psoriasis with comorbidities. Aims and Objectives: The objective of this study was to find out various comorbidities (abnormal body mass index, metabolic syndrome, lipid abnormalities, diabetes mellitus, and raised blood glucose) associated with paediatric psoriasis. Materials and Methods: All patients of psoriasis (age <18 years) who visited the Paediatric Psoriasis clinic from January 2017 to September 2021 were recruited in this record-based study. Results: Records of 100 patients were analysed, with female to male ratio of 1:1. The age group ranged from 11 months to 18 years. The average body surface area involved was 5.43%. The average psoriasis area and severity index was 2.47. Joint involvement was seen in 7% of patients in our study. A total of 52 (52%) patients had abnormal body mass index, out of which 21 patients were overweight and 31 were obese. At least one lipid abnormality was found in 66 (66%) patients. The most common lipid abnormality was decreased high-density lipoprotein, followed by raised total cholesterol, raised total triglycerides and raised low-density lipoprotein. 8 (8%) patients were found to have raised blood glucose levels. Out of which, six had impaired fasting glucose and two were diagnosed with diabetes mellitus. 5 (5%) patients were diagnosed with metabolic syndrome. Conclusion: Paediatric psoriasis is a chronic disfiguring disease and may have profound emotional and psychological effects. There is a lack of studies from India on the prevalence of these comorbidities in paediatric psoriasis. More studies are required from different parts of the world for a better understanding of paediatric psoriasis

Clinicoepidemiological profile of pediatric psoriasis: Experience from a tertiary care center

Introduction: Psoriasis is a common skin disorder and is relatively less studied in children. The objective of this study was to evaluate the clinical and epidemiological profile of pediatric psoriasis (PP). Materials and Methods: All patients of psoriasis (age 10 was seen in only 5% of patients. Joint involvement was found in 7% of patients and nail changes in 54%. Infection (15%) was the most common aggravating factor, followed by trauma and stress. Koebner phenomenon was present in 15% of patients. Family history of psoriasis was reported in 22% of the patients. A history of other autoimmune disease was present in 5 patients, out of which vitiligo and hypothyroidism was present in 2 patients each and 1 had morphea. Conclusion: PP is a persistent, chronic condition that can have significant psychosocial and emotional implications. For better understanding of PP, more population-based studies are required from different parts of the world

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N-Arylhydroxamic acids as a drug like molecule: A motif of binding mode with calf thymus DNA

215-221A drug-like molecule, which has a propensity of binding with DNA play a vital role in drug designing mechanism. In this paper, we tried to find out the DNA binding affinity of two derivatives of N-arylhydroxamic acids: (i) N-p-Chlorophenyl-2-methoxybenzohydroxamic Acid(Cl-2-MBHA) and (ii) N-p-Chlorophenyl-3-methoxybenzohydroxamic Acid (Cl-3-MBHA) with calf thymus DNA (ct-DNA) by applying techniques such as UV-visible spectroscopy, Fluorescence spectroscopy, and Viscometry measurements. The findings concluded with experimental techniques were verified with theoretical calculation using computer-based method, Molecular Docking. Absorption spectra revealed that both the hydroxamic acids derivatives bind to ct-DNA, among two, Cl-2-MBHA exhibits the higher value of binding affinity Kb (9.52 × 103±0.08 M-1). Fluorescence spectra showed that ct-DNA successfully quenches the emission spectra of N-arylhydroxamic acid. Ethidium bromide displacement method was used as a standard for analyzing the mode of binding. Both the hydroxamic acids were found to be groove binders. The Stern–Volmer Constant was found to be 2.05 × 10-2 ± 0.001 M-1 and 3.35 × 10-2 ± 0.002 M-1 for Cl-2-MBHA and Cl-3-MBHA respectively. Theoretical analysis molecular docking was done using Hex software for validating the experimental findings. Hence, it was observed that both experimental and computational method complimented the results and deduces groove binding as the mode of interaction

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Kanpur (26° 29' N 800 18' E) being located in central Uttar Pradesh enjoys semi-arid climate. Rice ( Oryza sativa) being miracle crop respond well to diverse agroecologicaUclimatic situation. It is its unique capacity to adopt in any situation, makes its wonderful crop of modem agriculture. Present day rice is grown from tropics to temperate condition, below sea level in Kerla to above 5,000 m in Nepal and handsomely adopted to problematic soils viz. acidic to saline alkaline. Being one of the most important cereals, every day rice is sown and harvested in the global village. Rice is staple food for South East Asian countries; this region itself is consuming almost 90% of rice production. India is a second largest rice producer in world following by China. Globally rice is grown on about 145 million hectares with a production of545 million tones. In India area under rice cultivation range 38 to 43 million hectares with a productiYity of 2.6 ton/ha only. The productivity is not satisfactory if we compare with.other producer like Egypt, Korea, Japan, Australia and China. The state of low productivity poses the challenge among the scientists to find out the causes and ways to remove them. Rice performance (growth, yiC!d component and yield) is determined by the numerous factors. Grain yield is one of the most complex polygenic traits, which is expected to show higher magnitude of interaction variance. Rice, a member of graminaei, interacts well with existing environment Planting time and selection of variety may play the deciding role in performance of rice, hence choice of correct variety and selection of right time of transplanting under particolar agroclimaticRice is major cereal crop of India and South Asia as well. To assess the response of prevailing environments and their interaction with photosensitive genotypes (mahsoori) and phptoinscnsitive (ashwani and pant-4). a field experiment was conducted under semiarid condition of Kanpur. This study proved that the year (1998) was more favorable for growth and development and by virtue of this fi rst year crops produced mon: iomass. It might be due to conducive environmental condit ion during vegetative phase and vice versa for eproductive phase. Moreover second year ( 1999) was better economic produce (grain yield), it may be because of ngenial environmental conditions during reproductive phase when despite low biomass efficient conversion of photosynthatcs from source to sink was observed. During both the years photoinscnsitive varieties viz. ashwani and pant-4 proved to be better. Delay in planting advanced the maturity significantly. Long duration genotype mahsoori recorded maximum plant height of 96.9 and 99.6 cm during the both years; 1,000-grain weight was not affected significantly due to environments, however significant diffcrct1ces were recorded due to genotypes during second year. Maximum above ground biomass production was recorded during first dates of planting ( IOS.2 and 98.S q/ha) and genotype mahsoori (106.4 and 9T.I q/ha). Harvest index being a typic al genotypic charac ter least affected by environmental fluctuations, variety ashwani produced boldest seed (28.0 and 28.4 g/l ,000 seeds) and mahsoori produced cylindrical Seeds with lowest seed weight (19.4 and 20.6 g). For successful rice ultivation under Kanpur condition, best time for transplanting is IS July selecting high yielding photoinscnsctive varieties.Not Availabl