21 research outputs found
The Physiological Race of the first Hemileia vastatrix (Coffee Leaf Rust) discovered in Hawaii
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
First report of the physiological race (XXIV) of Hemileia vastatrix (Coffee Leaf Rust) in Hawaii
This research was funded by the Foundation for Science and Technology (FCT) UNIT
(UID/AGR/04129/2020) of LEAF- -Linking Landscape, Environment, Agriculture and Food, Research Unit.info:eu-repo/semantics/publishedVersio
A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
A chromosome-level genome resource for studying virulence mechanisms and evolution of the coffee rust pathogen Hemileia vastatrix
Recurrent epidemics of coffee leaf rust, caused by the fungal pathogen Hemileia vastatrix, have
constrained the sustainable production of Arabica coffee for over 150 years. The ability of H.
vastatrix to overcome resistance in coffee cultivars and evolve new races is inexplicable for a
pathogen that supposedly only utilizes clonal reproduction. Understanding the evolutionary
complexity between H. vastatrix and its only known host, including determining how the
pathogen evolves virulence so rapidly is crucial for disease management. Achieving such goals
relies on the availability of a comprehensive and high-quality genome reference assembly. To
date, two reference genomes have been assembled and published for H. vastatrix that, while
useful, remain fragmented and do not represent chromosomal scaffolds. Here, we present a
complete scaffolded pseudochromosome-level genome resource for H. vastatrix strain 178a
(Hv178a). Our initial assembly revealed an unusually high degree of gene duplication (over
50% BUSCO basidiomycota_odb10 genes). Upon inspection, this was predominantly due to a
single scaffold that itself showed 91.9% BUSCO Completeness. Taxonomic analysis of
predicted BUSCO genes placed this scaffold in Exobasidiomycetes and suggests it is a distinct
genome, which we have named Hv178a associated fungal genome (Hv178a AFG). The high
depth of coverage and close association with Hv178a raises the prospect of symbiosis, although
we cannot completely rule out contamination at this time. The main Ca. 546 Mbp Hv178a
genome was primarily (97.7%) localised to 11 pseudochromosomes (51.5 Mb N50), building
the foundation for future advanced studies of genome structure and organization.info:eu-repo/semantics/publishedVersio
First report of a genome sequence resource of Colletotrichum kahawae, the causal agent of coffee berry disease
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
Potential association of HCF164, a chloroplast nuclear-encoded thioredoxin-like protein, with Coffea SH9 resistance factor against Hemileia vastatrix
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
Integrative transcriptomic and metabolomic approaches to unravel the resistance profile of Kawisari coffee against Hemileia vastatrix
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
Characterization of the transcriptional signatures associated with resistance and susceptibility to Hemileia vastatrix in the Kawisari coffee hybrid
Coffee leaf rust (CLR), a disease caused by the biotrophic fungus Hemileia vastatrix (Hv), is the main threat to the worldwide production of Arabica
coffee. The gradual breakdown of resistance in coffee varieties in the last years has highlighted the need for novel sources of resistance to CLR.
This work aimed to unveil the cellular and molecular resistance profile of the Kawisari hybrid (C. arabica x C. liberica), a genotype used as a
resistance donor in Arabica breeding programs in India. This coffee genotype was inoculated with two Hv races that triggered either resistance or
susceptibility. Progress of infection was monitored using light microscopy. Simultaneously, we conducted a time-course RNA-seq characterization
of the transcriptional responses. The microscopic studies showed that the post-haustorial resistance of Kawisari was associated with the
hypersensitive response, accumulation of phenolic-like compounds and haustorium encasement with callose. The transcriptomic analysis suggest
the downregulation of host primary metabolism genes at the early onset of infection, followed later by activation of genes functionally associated
with multiple plant defense responses, including salicylic acid and jasmonate hormonal signaling. Resistance was also accompanied by the
differential regulation of genes associated with phenylpropanoid metabolism and lignin biosynthesis. Our results, further validated by qPCR,
provide important new insight into the molecular mechanisms underpinning resistance against CLR in this coffee genotype.Foundation for Science and Technology (FCT) and FEDER funds through PORNorte under the project CoffeeRES PTDC/ASPPLA/
29779/2017 and by FCT UNIT LEAF (UID/AGR/04129/2020).info:eu-repo/semantics/publishedVersio
The story of coffee: legend and truth
FCT and FEDER funds refs. PTDC/ASP-PLA/32429/
2017 (POCI-01-0145-FEDER-032429) and PTDC/
ASP-PLA/29779/2017 and by FCT UNIT (UID/AGR/
04129/2020) of LEAF (Linking Landscape, Environment,
Agriculture and Food) Research Unit.info:eu-repo/semantics/publishedVersio