10 research outputs found
The influenza virus neuraminidase inhibitor produced by Staphylococcus aureus
The glycoproteinic complex has been isolated from the Staphylococcus aureus culture fluid possessing an activity inhibiting influenza virus neuraminidase. Two fractions have been further purified containing different monosugars components, the first one has been shown to contain mannose, glucose, rhamnose, glucosamine, and galactosamine, while rhamnose is absent in the second fraction, A component of fraction 1 inhibits the neuraminidase activity and at the same time enhances the virus hemagglutinating activity more strongly comparing to the fraction 2. Some chemical modifications of the substances of the fractions studied (periodate oxidation, protease pretreatment, and delipidization) prove the carbohydrate component of the complex to be responsible for its antineuraminidase activity. The protease pretreatment decreases twice the antineuraminidase activity of the fraction 2 having no influence on this activity of the fraction 1.Π ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ° Π·ΠΎΠ»ΠΎΡΠΈΡΡΠΎΠ³ΠΎ ΡΡΠ°ΡΡΠ»ΠΎΠΊΠΎΠΊΠ° (S. aureus) Π²ΠΈΠ΄ΡΠ»Π΅Π½ΠΎ Π³Π»ΡΠΊΠΎΠ»ΡΠΏΠΎΠΏΡΠΎΡΠ΅ΡΠ½ΠΎΠ²ΠΈΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ Π· Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ, ΡΠΎ Π³Π°Π»ΡΠΌΡΡ Π½Π΅ΠΉ ΡΠ°ΠΌΡΠ½ΡΠ΄Π°Π·Ρ Π²ΡΡΡΡΡ Π³ΡΠΈΠΏΡ. ΠΡΠΈΡΠ΅Π½ΠΎ Π΄Π²Ρ ΡΡΠ°ΠΊΡΡΡ Π· ΡΡΠ·Π½ΠΈΠΌ Π²ΠΌΡΡΡΠΎΠΌ ΠΌΠΎΠ½ΠΎΡΠ°Ρ
Π°ΡΠΈΠ΄ΡΠ². ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΠΎ ΡΡΠ°ΠΊΡΡΡ Π ΠΌΡΡΒΡΠΈΡΡ ΠΌΠ°Π½ΠΎΠ·Ρ, Π³Π»ΡΠΊΠΎΠ·Ρ, ΡΠ°ΠΌΠ½ΠΎΠ·Ρ, Π³Π»ΡΠΊΠΎΠ·Π°ΠΌΡΠ½ ΡΠ° Π³Π°Π»Π°ΠΊΡΠΎΠ·Π°ΠΌΡΠ½; Π΄ΡΡΠ³Π° β Π²ΡΡ ΡΡ ΠΌΠΎΠ½ΠΎΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΈ, ΠΊΡΡΠΌ ΡΠ°ΠΌΠ½ΠΎΠ·ΠΈ. Π Π΅ΡΠΎΠ²ΠΈΠ½Π° Π· ΡΡΠ°ΠΊΡΡΡ 1 Π³Π°Π»ΡΠΌΡΡ Π½Π΅ΠΉΡΠ°ΠΌΡΠ½ΡΠ΄Π°Π·Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠ° ΠΎΠ΄Π½ΠΎΡΠ°ΡΠ½ΠΎ ΠΏΠΎΡΠΈΠ»ΡΡ Π³Π΅ΠΌΠ°Π³Π»ΡΡΠΈΠ½Π°ΡΠΈΠ²Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ Π²ΡΡΡΡΡ Π·Π½Π°ΡΠ½ΠΎ ΡΠΈΠ»ΡΠ½ΡΡΠ΅, Π½ΡΠΆ ΡΠ΅ΡΠΎΠ²ΠΈΠ½Π° Π· ΡΡΠ°ΠΊΡΡΡ 2. Π ΡΠ΄ Ρ
ΡΠΌΡΡΠ½ΠΈΡ
ΠΌΠΎΠ΄ΠΈΡΡΠΊΠ°ΡΡΠΉ Π²ΠΈΠ²ΡΠ΅Π½ΠΈΡ
ΡΠ΅ΡΠΎΠ²ΠΈΠ½ (ΠΏΠ΅ΡΠΉΠΎΠ΄Π°ΡΠ½Π΅ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½Ρ, ΠΎΠ±ΡΠΎΠ±ΠΊΠ° ΠΏΡΠΎΡΠ΅Π°Π·ΠΎΡ ΡΠ° Π΄Π΅Π»ΡΠΏΡΠ΄ΠΈΠ·Π°ΡΡΡ) ΠΏΡΠ΄ΡΠ²Π΅ΡΠ΄ΠΆΡΡΡΡ ΡΠΎΠ»Ρ Π²ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ Ρ Π·Π΄ΡΠΉΡΠ½Π΅Π½Π½Ρ ΠΉΠΎΠ³ΠΎ Π°Π½ΡΠΈΠ½Π΅ΠΉΡΠ°ΠΌΡΠ½ΡΠ΄Π°Π·Π½ΠΎΡ Π΄ΡΡ; ΠΎΠ±ΡΠΎΠ±ΠΊΠ° ΠΏΡΠΎΡΠ΅Π°Π·ΠΎΡ Π²Π΄Π²ΡΡΡ Π·Π½ΠΈΠΆΡΡ Π°Π½ΡΠΈΠ½Π΅ΠΉΡΠ°ΠΌΡΠ½ΡΠ΄Π°Π·Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΡΠ°ΠΊΡΡΡ 2, Π½Π΅ Π²ΠΏΠ»ΠΈΠ²Π°ΡΡΠΈ Π½Π° ΡΠ°ΠΊΡ ΠΆ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΡΠ°ΠΊΡΡΡ 1.ΠΠ· ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Ρ S. aureus Π²ΡΠ΄Π΅Π»Π΅Π½ Π³Π»ΠΈΠΊΠΎΠ»ΠΈΠΏΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²ΡΠΉ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ, ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅ΠΉ Π½Π΅ΠΉΡΠ°ΠΌΠΈΠ½ΠΈΠ΄Π°Π·Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΠΈΡΡΡΠ° Π³ΡΠΈΠΏΠΏΠ°. ΠΠΎΠ»ΡΡΠ΅Π½Ρ Π΄Π²Π΅ ΡΡΠ°ΠΊΡΠΈΠΈ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΠΌΠΎΠ½ΠΎΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ², ΠΏΠ΅ΡΠ²Π°Ρ ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
Π²ΠΊΠ»ΡΡΠ°Π΅Ρ ΠΌΠ°Π½Π½ΠΎΠ·Ρ, Π³Π»ΡΠΊΠΎΠ·Ρ, ΡΠ°ΠΌΠ½ΠΎΠ·Ρ, Π³Π»ΡΠΊΠΎΠ·Π°ΠΌΠΈΠ½ ΠΈ Π³Π°Π»Π°ΠΊΡΠΎΠ·Π°ΠΌΠΈΠ½; Π²ΡΠΎΒΡΠ°Ρ β Π²ΡΠ΅ ΡΡΠΈ ΠΌΠΎΠ½ΠΎΡΠ°Ρ
Π°ΡΠΈΠ΄Ρ, ΠΊΡΠΎΠΌΠ΅ ΡΠΈΠΌΠ½ΠΎΠ·Ρ. ΠΠ΅ΡΠ΅ΡΡΠ²ΠΎ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠ΅Π΅ΡΡ Π²ΠΎ ΡΡΠ°ΠΊΡΠΈΠΈ I, ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅Ρ Π½Π΅ΠΉΡΠ°ΠΌΠΈΠ½ΠΈΠ΄Π°Π·Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Π² ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ ΡΡΠΈΠ»ΠΈΠ²Π°Π΅Ρ Π³Π΅ΠΌΠ°Π³Π³Π»ΡΡΠΈΠ½ΠΈΡΡΡΡΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΠΈΡΡΡΠ° Π³ΡΠΈΠΏΠΏΠ° Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΈΡ, ΡΠ΅ΠΌ Π²Π΅ΡΠ΅ΡΡΠ²ΠΎ ΠΈΠ· ΡΡΠ°ΠΊΡΠΈΠΈ 2. Π ΡΠ΄ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΉ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ
ΡΡΠ°ΠΊΡΠΈΠΉ (ΠΏΠ΅ΡΠΉΠΎΠ΄Π°ΡΠ½ΠΎΠ΅ ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΠ΅, ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΏΡΠΎΡΠ΅Π°Π·ΠΎΠΉ ΠΈ Π΄Π΅Π»ΠΈΠΏΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ) Π΄ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ ΡΠΎΠ»Ρ ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° ΠΊΠΎΠΌΒΠΏΠ»Π΅ΠΊΡΠ° Π² Π΅Π³ΠΎ Π°Π½ΡΠΈΠ½Π΅ΠΉΡΠ°ΠΌΠΈΠ½ΠΈΠ΄Π°Π·Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ; ΠΏΡΠΈ ΡΡΠΎΠΌ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΏΡΠΎΡΠ΅Π°Π·ΠΎΠΉ Π²Π΄Π²ΠΎΠ΅ ΡΠ½ΠΈΠΆΠ°Π΅Ρ Π°Π½ΡΠΈΠ½Π΅ΠΉΡΠ°ΠΌΠΈΠ½ΠΈΠ΄Π°Π·Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΡΠ°ΠΊΡΠΈΠΈ 2, Π½Π΅ Π²Π»ΠΈΡΡ Π½Π° ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΡΠ°ΠΊΡΠΈΠΈ 1
Characterization of lipids A of Ralstonia solanacearum lipopolysaccharides
The analysis of fatty acid profiles of lipopolysacharides has shown that R. solanacearum strains tested may be divided into two groups. The first group is represented by R. solanacearum strains (5712, 7945, 7955 and 8110) the lipids A of which contained hydroxylated fatty acids with long chains: 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic. The second group was represented by R. solanacearum strains the lipids A of which contained hydroxylated fatty acids with short chains: 3-hydroxydecanoic, 2-hydroxydodecanoic and 3-hydroxydodecanoic. 3-hydroxytetradecanoic acid was observed in a small amount. A comparative analysis of the fatty acid composition and biological activity gives a possibility to suppose that 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic acids may be responsible for the toxicity and pyrogenicity of the lipopolysaccharides tested.ΠΠ½Π°Π»ΡΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΈΡ
ΠΏΡΠΎΡΡΠ»ΡΠ² Π»ΡΠΏΠΎΠΏΠΎΠ»ΡΡΠ°Ρ
Π°ΡΠΈΠ΄ΡΠ² ΡΠ²ΡΠ΄ΡΠΈΡΡ ΠΏΡΠΎ ΡΠ΅, ΡΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Ρ ΠΈΡΡΠ°ΠΌΠΈ R. solanacearum ΠΌΠΎΠΆΠ½Π° ΠΏΠΎΠ΄ΡΠ»ΠΈΡΠΈ Π½Π° Π΄Π²Ρ Π³ΡΡΠΏΠΈ. ΠΠ΅ΡΡΠ° Π³ΡΡΠΏΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΡΡΠ°ΠΌΠ°ΠΌΠΈ R. solaΒnacearum (5712, 7945, 7955 Ρ 8110), Π»ΡΠΏΡΠ΄ΠΈ Π ΡΠΊΠΈΡ
ΠΌΡΡΡΡΡΡ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΠΈ Π· Π΄ΠΎΠ²Π³ΠΈΠΌΠΈ Π²ΡΠ³Π»Π΅ΡΠ΅Π²ΠΈΠΌΠΈ Π»Π°Π½ΡΡΠ³Π°ΠΌΠΈ: 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°-Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ ΡΠ° 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ. Π Π΄ΡΡΠ³Ρ Π³ΡΡΠΏΡ Π²Ρ
ΠΎΠ΄ΡΡΡ ΡΡΠ°ΠΌΠΈ R. solanacearum, Ρ Π»ΡΠΏΡΠ΄Π°Ρ
Π ΡΠΊΠΈΡ
ΠΏΡΠΈΡΡΡΠ½Ρ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΠΊΠΎΡΠΎΡΠΊΠΈΠΌΠΈ Π»Π°Π½ΡΡΠ³Π°ΠΌΠΈ: 3-ΠΎΠΊΡΠΈΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°, 2-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΡΠ° 3-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°. 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ ΠΊΠΈΡΠ»ΠΎΡΡ Π·Π½Π°ΠΉΠ΄Π΅Π½ΠΎ Π² Π½Π΅Π·Π½Π°ΡΠ½ΡΠΉ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ. ΠΠΎΡΡΠ²Π½ΡΠ»ΡΠ½ΠΈΠΉ Π°Π½Π°Π»ΡΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΄Π°Ρ ΠΏΡΠ΄ΡΡΠ°Π²Ρ ΠΏΡΠΈΠΏΡΡΡΠΈΡΠΈ, ΡΠΎ 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°-Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΡΠ° 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠΈ ΠΌΠΎΠΆΡΡΡ Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π°ΡΠΈ Π·Π° ΡΠΎΠΊΡΠΈΡΠ½ΡΡΡΡ ΡΠ° ΠΏΡΡΠΎΠ³Π΅Π½Π½ΡΡΡΡ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΈΡ
Π»ΡΠΏΠΎΠΏΠΎΠ»ΡΡΠ°ΒΡ
Π°ΡΠΈΠ΄ΡΠ².ΠΠ½Π°Π»ΠΈΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ
ΠΏΡΠΎΡΠΈΠ»Π΅ΠΉ Π»ΠΈΠΏΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ² Π²ΡΡΠ²ΠΈΠ», ΡΡΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ R. solanacearum ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ. ΠΠ΅ΡΠ²Π°Ρ Π³ΡΡΠΏΠΏΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΡΡΠ°ΠΌΒΠΌΠ°ΠΌΠΈ R. solanacearum (5712, 7945, 7955 ΠΈ 8110), Π»ΠΈΠΏΠΈΠ΄Ρ Π ΠΊΠΎΡΠΎΡΡΡ
ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΡ Ρ Π΄Π»ΠΈΠ½Π½ΡΠΌΠΈ ΡΠ΅ΠΏΡΠΌΠΈ: 3-ΠΎΠΊΡΠΈ-ΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ ΠΈ 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ. ΠΠΎ Π²ΡΠΎΡΡΡ Π³ΡΡΠΏΠΏΡ Π²Ρ
ΠΎΠ΄ΡΡ ΡΡΠ°ΠΌΠΌΡ R. solanacearum, Π² Π»ΠΈΠΏΠΈΠ΄Π°Ρ
Π ΠΊΠΎΡΠΎΡΡΡ
ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΡ Ρ ΠΊΠΎΡΠΎΡΠΊΠΈΠΌΠΈ ΡΠ΅ΠΏΡΠΌΠΈ: 3-ΠΎΠΊΡΠΈΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ, 2-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΈ 3-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ. 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π° Π² Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅. Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄Π°Π΅Ρ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΒΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΈ 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΌΠΎΠ³ΡΡ ΠΎΡΠ²Π΅ΡΠ°ΡΡ Π·Π° ΡΠΎΠΊΡΠΈΡΒΠ½ΠΎΡΡΡ ΠΈ ΠΏΠΈΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΠΏΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ²
Characterization of lipids A of Ralstonia solanacearum lipopolysaccharides
The analysis of fatty acid profiles of lipopolysacharides has shown that R. solanacearum strains tested may be divided into two groups. The first group is represented by R. solanacearum strains (5712, 7945, 7955 and 8110) the lipids A of which contained hydroxylated fatty acids with long chains: 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic. The second group was represented by R. solanacearum strains the lipids A of which contained hydroxylated fatty acids with short chains: 3-hydroxydecanoic, 2-hydroxydodecanoic and 3-hydroxydodecanoic. 3-hydroxytetradecanoic acid was observed in a small amount. A comparative analysis of the fatty acid composition and biological activity gives a possibility to suppose that 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic acids may be responsible for the toxicity and pyrogenicity of the lipopolysaccharides tested.ΠΠ½Π°Π»ΡΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΈΡ
ΠΏΡΠΎΡΡΠ»ΡΠ² Π»ΡΠΏΠΎΠΏΠΎΠ»ΡΡΠ°Ρ
Π°ΡΠΈΠ΄ΡΠ² ΡΠ²ΡΠ΄ΡΠΈΡΡ ΠΏΡΠΎ ΡΠ΅, ΡΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Ρ ΠΈΡΡΠ°ΠΌΠΈ R. solanacearum ΠΌΠΎΠΆΠ½Π° ΠΏΠΎΠ΄ΡΠ»ΠΈΡΠΈ Π½Π° Π΄Π²Ρ Π³ΡΡΠΏΠΈ. ΠΠ΅ΡΡΠ° Π³ΡΡΠΏΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΡΡΠ°ΠΌΠ°ΠΌΠΈ R. solaΒnacearum (5712, 7945, 7955 Ρ 8110), Π»ΡΠΏΡΠ΄ΠΈ Π ΡΠΊΠΈΡ
ΠΌΡΡΡΡΡΡ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΠΈ Π· Π΄ΠΎΠ²Π³ΠΈΠΌΠΈ Π²ΡΠ³Π»Π΅ΡΠ΅Π²ΠΈΠΌΠΈ Π»Π°Π½ΡΡΠ³Π°ΠΌΠΈ: 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°-Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ ΡΠ° 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ. Π Π΄ΡΡΠ³Ρ Π³ΡΡΠΏΡ Π²Ρ
ΠΎΠ΄ΡΡΡ ΡΡΠ°ΠΌΠΈ R. solanacearum, Ρ Π»ΡΠΏΡΠ΄Π°Ρ
Π ΡΠΊΠΈΡ
ΠΏΡΠΈΡΡΡΠ½Ρ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΠΊΠΎΡΠΎΡΠΊΠΈΠΌΠΈ Π»Π°Π½ΡΡΠ³Π°ΠΌΠΈ: 3-ΠΎΠΊΡΠΈΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°, 2-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΡΠ° 3-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°. 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Ρ ΠΊΠΈΡΠ»ΠΎΡΡ Π·Π½Π°ΠΉΠ΄Π΅Π½ΠΎ Π² Π½Π΅Π·Π½Π°ΡΠ½ΡΠΉ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ. ΠΠΎΡΡΠ²Π½ΡΠ»ΡΠ½ΠΈΠΉ Π°Π½Π°Π»ΡΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΄Π°Ρ ΠΏΡΠ΄ΡΡΠ°Π²Ρ ΠΏΡΠΈΠΏΡΡΡΠΈΡΠΈ, ΡΠΎ 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°-Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΡΠ° 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠΈ ΠΌΠΎΠΆΡΡΡ Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π°ΡΠΈ Π·Π° ΡΠΎΠΊΡΠΈΡΠ½ΡΡΡΡ ΡΠ° ΠΏΡΡΠΎΠ³Π΅Π½Π½ΡΡΡΡ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΈΡ
Π»ΡΠΏΠΎΠΏΠΎΠ»ΡΡΠ°ΒΡ
Π°ΡΠΈΠ΄ΡΠ².ΠΠ½Π°Π»ΠΈΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ
ΠΏΡΠΎΡΠΈΠ»Π΅ΠΉ Π»ΠΈΠΏΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ² Π²ΡΡΠ²ΠΈΠ», ΡΡΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ R. solanacearum ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ. ΠΠ΅ΡΠ²Π°Ρ Π³ΡΡΠΏΠΏΠ° ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΡΡΠ°ΠΌΒΠΌΠ°ΠΌΠΈ R. solanacearum (5712, 7945, 7955 ΠΈ 8110), Π»ΠΈΠΏΠΈΠ΄Ρ Π ΠΊΠΎΡΠΎΡΡΡ
ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΡ Ρ Π΄Π»ΠΈΠ½Π½ΡΠΌΠΈ ΡΠ΅ΠΏΡΠΌΠΈ: 3-ΠΎΠΊΡΠΈ-ΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ ΠΈ 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²ΡΡ. ΠΠΎ Π²ΡΠΎΡΡΡ Π³ΡΡΠΏΠΏΡ Π²Ρ
ΠΎΠ΄ΡΡ ΡΡΠ°ΠΌΠΌΡ R. solanacearum, Π² Π»ΠΈΠΏΠΈΠ΄Π°Ρ
Π ΠΊΠΎΡΠΎΡΡΡ
ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡ ΠΎΠΊΡΠΈΠΊΠΈΡΠ»ΠΎΡΡ Ρ ΠΊΠΎΡΠΎΡΠΊΠΈΠΌΠΈ ΡΠ΅ΠΏΡΠΌΠΈ: 3-ΠΎΠΊΡΠΈΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ, 2-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΈ 3-ΠΎΠΊΡΠΈΠ΄ΠΎΠ΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ. 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ° ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π° Π² Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅. Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΆΠΈΡΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄Π°Π΅Ρ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠ΅Π΄ΠΏΠΎΒΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ 3-ΠΎΠΊΡΠΈΡΠ΅ΡΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ, 2-ΠΎΠΊΡΠΈΠ³Π΅ΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΈ 2-ΠΎΠΊΡΠΈΠΎΠΊΡΠ°Π΄Π΅ΠΊΠ°Π½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΌΠΎΠ³ΡΡ ΠΎΡΠ²Π΅ΡΠ°ΡΡ Π·Π° ΡΠΎΠΊΡΠΈΡΒΠ½ΠΎΡΡΡ ΠΈ ΠΏΠΈΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
Π»ΠΈΠΏΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ²
Structure and gene cluster of the O antigen of E. Coli L-19, a candidate for a new O-serogroup
Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21
polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the
O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by
phenolβwater extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS.
The OPS was studied by sugar and methylation analyses, along with 1D and 2D 1
H and 13C NMR
spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to
be unique among known bacterial polysaccharide structures. A peculiar component of the L-19
OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol)
(GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was
sequenced, and gene functions were tentatively assigned by a comparison with sequences in the
available databases and found to be in agreement with the OPS structure. Except for putative
genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were
little related to those of reference strains of the 174 known E. coli O-serogroups. The data
obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup
Structure and gene cluster of the O antigen of E. Coli L-19, a candidate for a new O-serogroup
Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21
polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the
O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by
phenolβwater extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS.
The OPS was studied by sugar and methylation analyses, along with 1D and 2D 1
H and 13C NMR
spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to
be unique among known bacterial polysaccharide structures. A peculiar component of the L-19
OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol)
(GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was
sequenced, and gene functions were tentatively assigned by a comparison with sequences in the
available databases and found to be in agreement with the OPS structure. Except for putative
genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were
little related to those of reference strains of the 174 known E. coli O-serogroups. The data
obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup
Functional properties of individual sub-domains of the fibrin(ogen) Ξ±C-domains
Background: Fibrinogen is a large polyfunctional plasma protein consisting of a number of structural and functional domains. Among them, two Ξ±C-domains, each formed by the amino acid residues ΠΞ±392β610, are involved in fibrin polymerization, activation of fibrinolysis, platelet aggregation, and interaction with different cell types. Previous study revealed that each fibrinogen Ξ±C-domain consists of the N-terminal and C-terminal sub-domains. The major objections of the present study were to test functional role of these sub-domains in the above mentioned processes. Methods: To achieve these objections, we used specific proteases to prepare two truncated forms of fibrinogen, fibrinogen desAΞ±505β610 and fibrinogen desAΞ±414β610, missing their N-terminal and both N- and C-terminal sub-domains, respectively. Results: Our study with these truncated forms using turbidity measurements and electron microscopy revealed that the N- and C-terminal subdomains both contribute to protofibril formation and their lateral aggregation into fibers during fibrin polymerization process. These two sub-domains also contributed to platelet aggregation with the N-terminal sub-domains playing a more significant role in this process. At the same time, the C-terminal sub-domains make the major contribution to the plasminogen activation process. Further, our experiments revealed that the C-terminal sub-domains are involved in endothelial cell viability and migration of cancer cells. Conclusions: Thus, the results obtained establish the functional role of individual sub-domains of the Ξ±C-domains in fibrin polymerization, activation of fibrinolytic system, platelet aggregation, and cellular interactions. General significance: The present study expands our understanding of the functional role of individual fibrinogen domains and their specific portions in various fibrin(ogen)-dependent processes