Ceramic regenerator systems development program

The DOE/NASA Ceramic Regenerator Design and Reliability Program aims to develop ceramic regenerator cores that can be used in passenger car and industrial/truck gas turbine engines. The major cause of failure of early gas turbine regenerators was found to be chemical attack of the ceramic material. Improved materials and design concepts aimed at reducing or eliminating chemical attack were placed on durability test in Ford 707 industrial gas turbine engines late in 1974. Results of 53,065 hours of turbine engine durability testing are described. Two materials, aluminum silicate and magnesium aluminum silicate, show promise. Five aluminum silicate cores attained the durability objective of 10,000 hours at 800 C (1472 F). Another aluminum silicate core shows minimal evidence of chemical attack after 8071 hours at 982 C (1800 F). Results obtained in ceramic material screening tests, aerothermodynamic performance tests, stress analysis, cost studies, and material specifications are included

Dynamic interactions of a conserved enterotoxigenic Escherichia coli adhesin with intestinal mucins govern epithelium engagement and toxin delivery

At present, there is no vaccine for enterotoxigenic Escherichia coli (ETEC), an important cause of diarrheal illness. Nevertheless, recent microbial pathogenesis studies have identified a number of molecules produced by ETEC that contribute to its virulence and are novel antigenic targets to complement canonical vaccine approaches. EtpA is a secreted two-partner adhesin that is conserved within the ETEC pathovar. EtpA interacts with the tips of ETEC flagella to promote bacterial adhesion, toxin delivery, and intestinal colonization by forming molecular bridges between the bacteria and the epithelial surface. However, the nature of EtpA interactions with the intestinal epithelium remains poorly defined. Here, we demonstrate that EtpA interacts with glycans presented by transmembrane and secreted intestinal mucins at epithelial surfaces to facilitate pathogen-host interactions that culminate in toxin delivery. Moreover, we found that a major effector molecule of ETEC, the heat-labile enterotoxin (LT), may enhance these interactions by stimulating the production of the gel-forming mucin MUC2. Our studies suggest, however, that EtpA participates in complex and dynamic interactions between ETEC and the gastrointestinal mucosae in which host glycoproteins promote bacterial attachment while simultaneously limiting the epithelial engagement required for effective toxin delivery. Collectively, these data provide additional insight into the intricate nature of ETEC interactions with the intestinal epithelium that have potential implications for rational approaches to vaccine design

On the variability of I(7620 Å)/I(5577 Å) in low altitude aurora

International audienceAn auroral electron excitation model, combined with simple equilibrium neutral and ion chemistry models, is used to investigate the optical emission processes and height profiles of I(5577 Å) and I(7620 Å) in the 90 to 100 km altitude region. It is shown that the apparent discrepancies between ground-based and rocket-borne auroral observations of the I(7620 Å)/I(5577 Å) ratio are due to the extreme height variation of this intensity ratio in the 90 to 100 km region

The sustainability imperative and urban New Zealand : promise and paradox

'Urban sustainability' is an increasingly ubiquitous term now featuring in all manner of policy documents and promotional material. As an ambitious attempt to address social, economic and bio-physical environmental issues it appears to balance philanthropic ideals, such as social development, with environmental concern and fiscal efficiency. Yet, my research involving in-depth interviews with 35 urban practitioners in Christchurch, New Zealand, exposes much of the apparent consensus around its meaning as illusory. Though the concept's promise rests on an apparently neutral reconciliation of disparate goals and aspirations, it is conceptually paradoxical, difficult to implement and extremely political. While the orthodox tripartite promotes a combination of social, economic and environmental elements, I have found practitioners tend to emphasise bio-physical aspects of the concept. As a corollary, urban sustainability is often reified as a technical problem to be managed within certain budget constraints. The ways in which the concept is quite literally made concrete in our cities and towns naturalises certain social arrangements, such as, for example, the spatial segregation of different groups. The processes of reification also serve to legitimise particular rationalities, one of which encourages a particular reading of 'the environment' that rests on an unhelpful and possibly dangerous separation of nature and the city. In this thesis I use techniques associated with discourse analysis and symbolic interaction, informed by an eclectic literature around social geography, and urban political economy and ecology, to explore and elaborate upon these themes

Testing a Grassroots Citizen Science Venture Using Open Design, “the Bee Lab Project”

The Bee Lab project applies Citizen Science and Open Design to beekeeping, enabling participants to construct monitoring devices gathering reciprocal data, motivating participants and third parties. The presented approach uses design workshops to provide insight into the design of kits, user motivations, promoting reciprocal interests and address community problems. This paper signposts issues and opportunities in the process of designing Citizen Science tools for communities using Open Design to solve individual problems, including: downloadable design for social/local change, laypeople creating technology and repairable kits

Repurposing cancer drugs, batimastat and marimastat, to inhibit the activity of a group I metalloprotease from the venom of the Western Diamondback rattlesnake, Crotalus atrox

Snakebite envenomation causes over 140,000 deaths every year predominantly in developing countries. As a result, it is one of the most lethal neglected tropical diseases. It is associated with an incredibly complex pathophysiology due to the vast number of unique toxins/proteins found in the venoms of diverse snake species found worldwide. Here, we report the purification and functional characteristics of a group I metalloprotease (CAMP-2) from the venom of the western diamondback rattlesnake, Crotalus atrox. Its sensitivity to matrix metalloprotease inhibitors (batimastat and marimastat) was established using specific in vitro experiments and in silico molecular docking analysis. CAMP-2 shows high sequence homology to atroxase from the venom of Crotalus atrox and exhibits collagenolytic, fibrinogenolytic and mild haemolytic activities. It exerts a mild inhibitory effect on agonist-induced platelet aggregation in the absence of plasma proteins. Its collagenolytic activity was completely inhibited by batimastat and marimastat. Zinc chloride also inhibits the collagenolytic activity of CAMP-2 by around 75% at 50 M, while it is partially potentiated by calcium chloride. Molecular docking studies demonstrate that batimastat and marimastat are able to bind strongly to the active site residues of CAMP-2. This study demonstrates the impact of matrix metalloprotease inhibitors in the modulation of a purified, group I metalloprotease activities in comparison to the whole venom. By improving our understanding of snake venom metalloproteases and their sensitivity to small molecule inhibitors, we can begin to develop novel and improved treatment strategies for snakebites