76 research outputs found
Three new triterpenes from the resinous bark of Protium kleinii and their antinociceptive activity
Three novel pentacyclic triterpenes have been isolated from the resin of Protium kleinii and their structures elucidated by NMR spectroscopy as 3-oxo-11beta,16beta-dihydroxy-urs-12-ene (1), 3-oxo-11beta-hydroxy-urs-12-ene (2) and 3-oxo-11beta-hydroxy-olean-12-ene (3). The systemic administration of the above compounds (10-100 mg kg-1, i.p.) was able to inhibited both first and second phase of the formalin-induced nociception in mice. The known triterpenes 3beta,16beta-dihydroxy-olean-12-ene (brein) (4) and a mixture of alpha (5)- and beta-amyrin (6) were also found. Três triterpenos pentacíclicos inéditos foram isolados da resina de Protium kleinii. Suas estruturas, 3-oxo-11beta,16beta-diidróxi-urs-12-eno (1), 3-oxo-11beta-hidróxi-urs-12-eno (2) e 3-oxo-11beta-hidróxi-olean-12-eno (3), foram elucidadas por espectroscopia de RMN. A administração sistêmica destes compostos (10-100 mg kg-1, i.p.) foi capaz de inibir tanto a primeira quanto a segunda fase da nocicepção induzida pela formalina em camundongos. Em adição, foram isolados os triterpenos conhecidos 3beta,16beta-dihidróxi-urs-12-eno (breina) (4) e uma mistura de alfa (5)- e beta-amirina (6)
Three new triterpenes from the resinous bark of Protium kleinii and their antinociceptive activity
Pharmacological and Phytochemical Evaluation of Adiantum cuneatum Growing in Brazil
This work describes the phytochemical analysis and analgesic activity of a non polar fraction obtained from Adiantum cuneatum grown in Brazil. The results showed that the hexane fraction as well as two pure compounds, identified as filicene (1) and filicenal (2), given intraperitoneally, exhibited potent analgesic activity when evaluated in two models of pain in mice, writhing test and formalin-induced pain. Compound 1 presented a calculated ID50 value of 19.5 μmol/kg body weight, when evaluated in writhing test, being about 7-fold more active than some reference drugs, like as acetyl salicylic acid and acetaminophen. It also inhibited both phases (neurogenic and inflammatory) of the formalin test at 10 mg/kg (24 μmol/kg). The chemical composition of the plant grown in Brazil is similar to that grown in other countries. The results confirm and justify the popular use of this plant for the treatment of dolorous processes
Extrato de plantas medicinais como carrapaticida de Rhipicephalus (Boophilus) microplus
INVESTIGAÇÃO FITOQUÍMICA E AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA DA Mikania lanuginosa DC (ASTERACEAE)
O presente trabalho envolveu extração, isolamento, purificação e identificação dos constituintes
químicos das partes aéreas da Mikania lanuginosa DC (ASTERACEAE). No processo de extração
ocorreu a formação de um precipitado, no qual foi isolado e purificado um composto majoritário,
que por análises espectroscópicas de IV, RMN 1H, RMN 13C e por comparação com os dados
existentes na literatura, foi identificado como sendo o diterpeno ácido caurenóico. Foram realizados
ensaios biológicos para avaliar a ação antimicrobiana com o precipitado, extrato etanólico bruto,
as frações hexânica, diclorometano, acetato de etila, butanólica e o diterpeno ácido caurenóico
contra as cepas Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus
aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, pela técnica da
difusão em meio sólido por cavidade e posteriormente pelo método de diluição em placa. Os
resultados demonstraram que as frações hexânica, diclorometano, acetato de etila, butanólica e
o ácido caurenóico obtidos da M. lanuginosa, apresentaram atividade antimicrobiana moderada
frente as bactérias Gram positivas e foram inativos contra as bactérias Gram negativas.
Phytochemical Investigation and Antimicrobial Activity Evaluation of the Mikania
lanuginosa DC (ASTERACEAE)
Abstract
The present study investigated the extraction, isolation and purification the chemical compounds of
the aerial parts of Mikania lanuginosa DC (ASTERACEAE). In the process of extraction it occurred the
formation of a precipitate, it which was isolated and purified a ruling compound, and by spectroscopic
analysis of the IR, 1H NMR, 13C NMR and comparison with reported data in the literature it was identified
as being the diterpene kaurenoic acid. It were performed biological assays to estimate the
antimicrobial action with the precipited, crude etanolic extract, hexane, dichoromethane, acetate
ethyl, butane fractions and diterpene kaurenoic acid contrasting with strains Escherichia coli ATCC
25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus
epidermidis ATCC 12228 e Bacillus cereus, by technique of the diffusion in solid mean by cavity and
afterwards by the plaque dilution method. The results showed that the hexane, dichoromethane,
acetate ethyl, butane fractions and the kaurenoic acid obtained from the Mikania lanuginosa DC
presented a moderate antimicrobial activity face to Gram positives bacteria and got inert against
the Gram negatives bacteria
Toxicological and pharmacological evaluation of Discaria americana Gillies & Hook (Rhamnaceae) in mice
Characterization of the molluscicidal activity of Bauhinia variegata and Mimusops elengi plant extracts against the fasciola vector lymnaea acuminata
Preparo e caracterização de tinturas das folhas de chá verde [Camellia sinensis (L.) O. Kuntze] Theaceae
Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE)
Estudo comparativo dos efeitos do extrato de Ginkgo biloba L. e Panax ginseng C.A. Meyer na reprodução de ratos machos e fêmeas Wistar
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