The degree of mutual anisotropy of biological liquids polycrystalline nets as a parameter in diagnostics and differentiations of hominal inflammatory processes

To characterize the degree of consistency of parameters of the optically uniaxial birefringent protein nets of blood plasma a new parameter – complex degree of mutual anisotropy is suggested. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of blood plasma is developed. It is shown that statistic approach to the analysis of the complex degree of mutual anisotropy distributions of blood plasma is effective during the diagnostics and differentiation of an acute inflammatory processes as well as acute and gangrenous appendicitis

CLASSIFYING OPTICAL PROPERTIES OF SURFACE- AND BULK-SCATTERING BIOLOGICAL LAYERS WITH POLARIZATION SINGULAR STATES

The results of singular approach usage in the tasks of description and classification of appearance of optical anisotropy of di®erent types of phase-inhomogeneous biological layers (surfacescattering, optically thin and optically thick) have been presented. The characteristic values of the fourth Stokes vector parameter (S4=0 — linear polarization — (L-state); S4= ±1 — circular polarization — ( ±C-state)) have been chosen as the main analytical tool describing polarization-singular states. The value of S4 has been determined by the value of phase shift between the orthogonal components of amplitude in the point of biological layer laser image and therefore is azimuthally stable. Hence, statistic moments of the first to the fourth orders characterizing the distribution of the amount of characteristic values S4=0; S4=± 1 have been used for definition and differentiation of optical properties of different types of biological layers — surface scattering, optically thin and optically thick human skin

Birefringence images of polycrystalline films of human urine in early diagnostics of kidney pathology

We propose an optical model of the Mueller-matrix description of mechanisms of optical anisotropy of polycrystalline films of urine, namely, optical activity and birefringence. The algorithm of reconstruction of distributions of parameters-optical rotation angles and phase shifts of the indicated anisotropy types-are elaborated upon. The objective criteria of differentiation of urine films taken from healthy donors and albuminuria patients by means of statistical analysis of such distributions are determined. The operational characteristics (sensitivity, specificity, and accuracy) of the Mueller-matrix reconstruction method of the optical anisotropy parameters are defined

Degree of local depolarization determined for fields of laser radiation scattered by multilayer birefringent networks of protein crystals

Represented in this work are theoretical basics for description of fields created by scattered coherent radiation with using the new correlation parameter – degree of local depolarization (DLD). The authors have adduced data of measurements of coordinate distributions for DLD in laser images of healthy and pathologically changed skin of a rat. Investigated are the values and ranges of changes in statistical (moments of the first to fourth orders), correlation (correlation area) and fractal (slopes and dispersion of extremes of logarithmic dependences for power spectra) parameters of coordinate distributions for DLD. Defined are objective criteria for diagnostics of oncological changes in the structure of rat skin

New parameter for describing and analyzing the optical-anisotropic properties of biological tissues

To characterize the degree of similarity inherent to parameters of the optically uniaxial birefringent protein-¯bril networks of biological tissues, a new parameter — complex degree of mutual anisotropy — has been o®ered. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues has been developed. It has been shown that statistical approach to the analysis of complex degree of mutual anisotropy distributions for biological tissues in various morphological and physiological states and for di®erent optical thicknesses appears to be more sensitive and e±cient in di®erentiation of physiological state, as compared to investigations of complex degree of mutual polarization in the corresponding laser images

The Interconnection between the Coordinate Distribution of Mueller-Matrixes Images Characteristic Values of Biological Liquid Crystals Net and the Pathological Changes of Human Tissues

We have theoretically grounded conceptions of characteristic points observed in coordinate distributions of Mueller matrix elements for a network of human tissue biological crystals. The interrelation between polarization singularities of laser images inherent to these biological crystals and characteristic values of above matrix elements is found. We have determined the criteria for statistical diagnostics of pathological changes in the birefringent structure of biological crystal network by using myometrium tissue as an example

Jones-matrix mapping of complex degree of mutual anisotropy of birefringent protein networks during the differentiation of myocardium necrotic changes

Spectral methods based on investigation of absorption spectra of biological objects and media. Polarization methods. The main disadvantage of such a mehtods consists in the azimuthal dependence of the obtained data on the rotation angle of the sample in relation to the probing beam. Therefore the results of polarization mapping are hardly reproducible. Correlation methods are devoid of this disadvantage

LASER POLARIZATION-VARIABLE AUTOFLUORESCENCE OF THE NETWORK OF OPTICALLY ANISOTROPIC BIOLOGICAL TISSUES: DIAGNOSTICS AND DIFFERENTIATION OF EARLY STAGES OF CANCER OF CERVIX UTERI

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and differentiation of early stages of cancer: Dysplasia — cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (fibrillar, porphyrin) networks. The technique of polarization-variable laser autofluorescence was developed and experimentally tested. The objective criteria (statistical moments) of differentiation of histological sections autofluorescent images of endometrium biopsy and cytological smears of it mucous coat were defined. The operational characteristics (sensitivity, specificity, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical efficiency

Polarization Singular Structure of Laser Images of Stratified Phase Inhomogeneous Layers for the Diagnostics and Classification of their Optical Properties

Presented in this work are the results of investigation aimed at analysis of coordinate distri butions for azimuths and ellipticity of polarization (polarization maps) in laser images of three types of phase inhomogeneous layers, namely: rough, ground and bulk scattering layers. To characterize polarization maps for all the types of phase inhomogeneous layers, the authors have offered to use three groups of parameters: statistical moments of the first to the fourth orders, autocorrelation func tions and logarithmic dependences for power spectra related to distributions of azimuths and ellipticity of polarization inherent to phase inhomogeneous layers laser images. Ascertained are the criteria for diagnostics and classification of phase inhomogeneous layers optical properties

Complex degree of coherency inherent to laser images of blood plasma polycrystalline networks

Given in this paper are theoretical basics for correlation-phase analysis of laser images inherent to human blood plasma. Also presented are comparative results of measurements aimed at coordinate distributions of the module of complex degree of coherency (CDC) and complex degree of mutual polarization (CDMP) of laser images describing blood plasma of a healthy person as well as of a patient with prostate cancer of the first stage. The authors investigated both values and ranges of changing the statistical (moments of the first to fourth orders), correlation (coefficients of the GrammCharlie expansion for autocorrelation functions) and fractal (slopes and dispersion of extremes for logarithmic dependences of power spectra) parameters for coordinate distributions CDC and CDMP. Determined are objective criteria for diagnostics of cancer changes in blood plasma of a patient with cancer