Effects of copper oxide nanoparticles on tissue accumulation and antioxidant enzymes of galleria mellonella L

Effects of copper oxide nanoparticles (CuO NPs) were investigated in the midgut and fat body of Galleria mellonella. Fourth instar larvae were exposed to 10 µg Cu/L of CuO until becoming last instar larvae, and catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) and acetylcholinesterase (AChE) and metal accumulation were evaluated. Copper accumulation was observed in midgut and fat body tissues of G. mellonella larvae exposed to CuO NPs. CuO NPs increased CAT activities in midgut and fat body, while SOD activities were decreased. CuO NPs exhibited significant increases in GST activity in fat body, while no significant differences were observed in the midgut of G. mellonella larvae. AChE activity significantly decreased in the midgut of G. mellonella whereas there is no significant effect on fat body in CuO NPs exposed larvae. In overall, these findings demonstrate that tissue accumulation and oxidative stress that is countered by antioxidant enzymes occur when G. mellonella larvae exposed to environmental concentration of CuO nanoparticles.info:eu-repo/semantics/publishedVersio

Accumulation of copper oxide nanoparticles in gill, liver and muscle tissues of Clarias gariepinus

53rd Congress of the European-Societies-of-Toxicology (EUROTOX) -- SEP 10-13, 2017 -- Bratislava, SLOVAKIAWOS: 000425486700449…European Soc ToxicolCukurova University, Scientific Research Projects Coordination UnitCukurova UniversityThis work was supported by Cukurova University, Scientific Research Projects Coordination Unit

METAL ACCUMULATION IN VARIOUS TISSUES OF Clarias gariepinus EXPOSED TO COPPER, ZINC, CADMIUM AND LEAD SINGLY AND IN MIXTURE

WOS: 000367699500005Accumulation of copper, zinc, cadmium and lead in gill, liver, spleen, kidney and muscle tissues of Clarias gariepinus was studied after exposing the fish to 5 ppm Cu, 5 ppm Zn, 1 ppm Cd, 1 ppm Pb and to the same concentrations of their mixture over 1, 7 and 15 days. Atomic absorption techniques were applied in determining tissue metal levels. Highest Cu and Zn accumulation was observed in liver and kidney tissues at all exposure periods whereas highest Cd and Pb accumulation was in gill and liver tissues. The lowest metal accumulation was observed in muscle tissue. Accumulation of these metals in the tissues studied increased compared to control when exposed singly. Tissue accumulation of metals, however, decreased when exposed to metals in mixture compared with single exposures to metals. It was concluded that exposure to these metals in mixture had an antagonistic effect on metal accumulation