Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis

The article presents the results of studying the role of erythrocyte and leukocyte indices of intoxication in the pathogenesis of experimental peritonitis. When studying the role of erythrocyte and leukocyte indices, a unidirectional trend of their change was revealed. An increase in the erythrocyte index of intoxication in the pathogenesis of experimental fecal peritonitis was established, most pronounced on the 10th day of the experiment (the level of EII increased by 107% compared to intact animals). The results of the study of the leukocyte intoxication index indicate a unidirectional trend, as in the case of the erythrocyte intoxication index. On the first day, the leukocyte index of intoxication increased on 225% (р<0.05), on the 4th day – on 256.7% (р<0.05), and on the 10th day – on 260% (р <0.05) compared to intact animals. It has been proven that the leukocyte index of intoxication is a more sensitive marker compared to the erythrocyte index

Research of effects by donators nitrogen of oxygen in complex therapy during experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups:Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis.Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction  correction with the use of a nitric oxide donor.Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per100 gof animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826).The following results were obtained.Histologically confirmed the development of experimental peritonitis in all groups of the experiment. In the group №2 on the third day the deterioration of the studied structures was established. In the group in which the experimental peritonitis was corrected by antibiotic therapy and chlorhexidine remediation, the effectiveness of the correction was morphologically proven. In group 4, macroscopic and microscopic examinations of the intestine, liver, and abdomen did not reveal significant differences from the histological picture we observed in rats of the intact group

Vasoconsrictive and vasodilatative imbalance at experimental fecal peritonitis

Peritonitis problem today one of the most urgent issues in the field of clinical pathological physiology and abdominal surgery. According to various data, mortality in peritonitis is 18.3 - 62.8%. Endothelial dysfunction is the main cause of cardiovascular diseases and death in patients after postponed peritonitis. The aim of the study – investigation of vasoconstrictive and vasodilatative potential in the pathogenesis of experimental peritonitis and its correction. It has been proved that experimental fecal peritonitis development pathologically affects the vessels functional state at each of the research stages. It's established weakening of the vasodilatative process on the background of peritonitis development, as evidenced by a decrease in the content of S-nitrosothiols. Result of this work has been proved that in peritonitis pathogenesis the vasoconstrictor potential of the vessels is significantly increased, as evidenced by a significant increase in endothelin-1 in animals that have been modeled by fecal peritonitis. Nitric oxide donor efficiency has been proved as part of complex correction of peritonitis and endothelial dysfunction as its complication. The most pronounced positive effect was detected at the 21st day, which indicates the expediency of long-term use of L-arginine as a remedy for correction

Vasoconsrictive and vasodilatative imbalance at experimental fecal peritonitis

Peritonitis problem today one of the most urgent issues in the field of clinical pathological physiology and abdominal surgery. According to various data, mortality in peritonitis is 18.3 - 62.8%. Endothelial dysfunction is the main cause of cardiovascular diseases and death in patients after postponed peritonitis. The aim of the study – investigation of vasoconstrictive and vasodilatative potential in the pathogenesis of experimental peritonitis and its correction. It has been proved that experimental fecal peritonitis development pathologically affects the vessels functional state at each of the research stages. It's established weakening of the vasodilatative process on the background of peritonitis development, as evidenced by a decrease in the content of S-nitrosothiols. Result of this work has been proved that in peritonitis pathogenesis the vasoconstrictor potential of the vessels is significantly increased, as evidenced by a significant increase in endothelin-1 in animals that have been modeled by fecal peritonitis. Nitric oxide donor efficiency has been proved as part of complex correction of peritonitis and endothelial dysfunction as its complication. The most pronounced positive effect was detected at the 21st day, which indicates the expediency of long-term use of Larginine as a remedy for correction

Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups: Group 1 - 20 intact animals. Group 2 - 50 rats with simulated fecal peritonitis. Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor. Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826). The following results were obtained. Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis. Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001). It was revealed significant increase in erythrocyte intoxication index (EII). It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis. It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed

Прогностическая модель развития эндотелиальной дисфункции при экспериментальном перитоните

Для прогнозування виникнення ендотеліальної дисфункції (ЕД) використовувався метод логістичної регресії. У якості факторів-предикторів були використані лейкоцитарний та еритроцитарний індекси інтоксикації і фактор Віллебранда. В результаті дослідження було встановлено, що розроблена модель в 91,30% випадків вірно передбачає виникнення ЕД. Відсоток хибних передбачень складає 8,70%. При цьому в 92,59% випадків модель вірно передбачає відсутність виникнення ЕД, частка хибних передбачень складає відповідно 7,41%. Можемо стверджувати, що така якість прогнозу дозволяє використання даної моделі у практиці. Ключові слова: перитоніт, експеримент, прогностична модель, ендогенна інтоксикація, ендотеліальна дисфункціяWe used logistic regression method to predict occurrence on endothelial dysfunction (ED).The following factors were used as predictors: leukocyte index on intoxication (LII), erythrocyte index on intoxication (EII) and von Willebrand Factor (VWF). It was found that the prognostic model developed by us in 91.30% on cases correctly suggests the emergence on ED. In 92.59% on cases the model correctly assumes the absence on ED, the proportion on false predictionsis is 7.41%. This prognosis quality allows us use this model in practice.Для прогнозирования возникновения эндотелиальной дисфункции использовался метод логистической регрессии. В качестве факторов-предикторов были использованы лейкоцитарный и эритроцитарный индексы интоксикации и фактор Виллебранда. В результате исследования было установлено, что разработанная модель в 91,30% случаев верно предполагает возникновение эндотелиальной дисфункции.Процент ложных предсказаний составляет 8,70%. При этом в 92,59% случаев модель верно предполагает отсутствие возникновения ЭД, доля ошибочных предсказаний составляет соответственно 7,41%. Можем утверждать, что такое качество прогноза позволяет использование данной модели на практике

Research of effects by donators nitrogen of oxygen in complex therapy during experimental peritonitis

The research was conducted on 175 white rats of reproductive age (3 months), weight of animals – 180–220 g. Animals were divided into 4 groups: Group 1 – 20 intact animals. Group 2 – 50 rats with simulated fecal peritonitis. Group 3 – 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution. Group 4 – 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor.Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826). The following results were obtained. Histologically confirmed the development of experimental peritonitis in all groups of the experiment. In the group №2 on the third day the deterioration of the studied structures was established. In the group in which the experimental peritonitis was corrected by antibiotic therapy and chlorhexidine remediation, the effectiveness of the correction was morphologically proven. In group 4, macroscopic and microscopic examinations of the intestine, liver, and abdomen did not reveal significant differences from the histological picture we observed in rats of the intact group

Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis

Gutsulyuk V. G., Savytskyi I. V., Tsypoviaz S. V., Znamerovskyi S. G., Zashchuk R. G. Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis. Journal of Education, Health and Sport. 2022;12(4):341-346. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.04.029 https://apcz.umk.pl/JEHS/article/view/41779 https://zenodo.org/record/7514133 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 23.03.2022. Revised: 04.04.2022. Accepted: 29.04.2022. UDC 616-092:616-036.8 Study of the role of erythrocyte and leukocyte intoxication indexes in the pathogenesis of experimental peritonitis V. G. Gutsulyuk, I. V. Savytskyi, S. V. Tsypoviaz, S. G. Znamerovskyi, R. G. Zashchuk International Academy of Ecology and Medicine, Kyiv, Ukraine Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road4-а/29, tel.+38050-381-21-83, [email protected] Abstract The article presents the results of studying the role of erythrocyte and leukocyte indices of intoxication in the pathogenesis of experimental peritonitis. When studying the role of erythrocyte and leukocyte indices, a unidirectional trend of their change was revealed. An increase in the erythrocyte index of intoxication in the pathogenesis of experimental fecal peritonitis was established, most pronounced on the 10th day of the experiment (the level of EII increased by 107% compared to intact animals). The results of the study of the leukocyte intoxication index indicate a unidirectional trend, as in the case of the erythrocyte intoxication index. On the first day, the leukocyte index of intoxication increased on 225% (р<0.05), on the 4th day – on 256.7% (р<0.05), and on the 10th day – on 260% (р <0.05) compared to intact animals. It has been proven that the leukocyte index of intoxication is a more sensitive marker compared to the erythrocyte index. Keywords: peritonitis; leukocyte index of intoxication; erythrocyte index of intoxication; pathogenesis

Study of indicators of endothelial dysfunction in rats with experimental peritonitis

Gutsulyuk V. G., Savytskyi I. V., Tsypoviaz S. V., Znamerovskyi S. G., Sarahan V. M., Gerasymenko T. V. Study of indicators of endothelial dysfunction in rats with experimental peritonitis. Journal of Education, Health and Sport. 2022;12(1):514-522. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.01.043 https://apcz.umk.pl/JEHS/article/view/41740 https://zenodo.org/record/7512638 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.01.2022. Revised: 21.01.2022. Accepted: 31.01.2022. UDC 616.381-018.74 Study of indicators of endothelial dysfunction in rats with experimental peritonitis V. G. Gutsulyuk, I. V. Savytskyi, S. V. Tsypoviaz, S. G. Znamerovskyi, V. M. Sarahan, T. V. Gerasymenko International Academy of Ecology and Medicine, Kyiv, Ukraine Correspondence author: Savytskyi Ivan Volodymyrovich, 65039, Odesa, Fountain road4-а/29, tel.+38050-381-21-83, [email protected] Abstract The article presents the results of the study of the activity of endothelial and inducible NO-synthase, the level of Willebrand factor and endothelin-1 in rats with experimental peritonitis. The most likely mechanism that is damaged in the endothelium during peritonitis is the activation of the synthesis of inducible NO-synthase by neutrophils/macrophages in response to infection. It is possible that hyperproduction of nitric oxide (NO), on the one hand, is aimed at destroying microflora and oxidizing toxins, and on the other hand, at suppressing the expression of tissue factor and cell adhesion molecules. platelet aggregation and cascade disorders in the hemostasis system. All this indicates that the hyperproduction of NO not only reflects the processes that occur in the focus of damage to the vascular endothelium, but also affects the severity of the inflammatory process and the outcome of the disease. In animals with experimental peritonitis on the background of OS, an increase in the number of circulating desquamated endothelial cells in the blood, which is a highly specific marker of endothelial dysfunction, was noted. The level of the Willebrand factor also increased, which can serve as a marker of increased risk of thrombus formation and indicate the pathogenetic dependence of the factors that damage the vascular wall endothelium on the concentration of the Willebrand factor, which contributes to the reduction of vascular permeability by adhesion of platelets to the endothelium. Confirmation of the development of endothelial dysfunction in peritonitis is an increase in the concentration of endothelin-1, which is a regulator of the process of vascular neoangiogenesis in response to endothelial damage. Keywords: peritonitis; endothelial dysfunction; oxidative stress; Willebrand factor; pathogenesis

Dynamics of vasoconstructor-vasodilation potential on the background of the development of experimental diabetic retinopathy

Proved development of endothelial dysfunction in rats that was modeled experimental diabetic retinopathy, as evidenced by increased endothelin-1 levels. It was found that at each stage of the study, pathological vasoconstriction increases (p <0.001 compared with the intact group). It was revealed that simulated diabetic retinopathy negatively affects vasodilation potential, as evidenced by the analysis of the content of S-nitrosothiols in the blood of experimental animals (p <0.001 compared with the data of intact animals). In the analysis of vasoconstrictor-vasodilyatatsiynogo potential at each stage of the experiment proved the prevalence of pathological vasoconstriction (p <0.001). The weakening of the vasodilatory potential was step by step more gradual in comparison w ith the dynamics of the increase in the vasodilation potential. In a comparative experiment characteristic pathological stages most pronounced shifts mounted on stage III of diabetic retinopathy study simulated (180-day)