Interplay between Chromatin and trans-Acting Factors on the IME2 Promoter upon Induction of the Gene at the Onset of Meiosis

The IME2 gene is one of the key regulators of the initiation of meiosis in budding yeast. This gene is repressed during mitosis through the repressive chromatin structure at the promoter, which is maintained by the Rpd3-Sin3 histone deacetylase (HDAC) complex. IME2 expression in meiosis requires Gcn5/histone acetyltransferase, the transcriptional activator Ime1, and the chromatin remodeler RSC; however, the molecular basis of IME2 activation had not been previously defined. We found that, during mitotic growth, a nucleosome masked the TATA element of IME2, and this positioning depended on HDAC. This chromatin structure was remodeled at meiosis by RSC that was recruited to TATA by Ime1. Stable tethering of Ime1 to the promoter required the presence of Gcn5. Interestingly, Ime1 binding to the promoter was kept at low levels during the very early stages in meiosis, even when the levels of Ime1 and histone H3 acetylation at the promoter were at their highest, making a 4- to 6-h delay of the IME2 expression from that of IME1. HDAC was continuously present at the promoter regardless of the transcriptional condition of IME2, and deletion of RPD3 allowed the IME2 expression shortly after the expression of IME1, suggesting that HDAC plays a role in regulating the timing of IME2 expression

Identification of a wheat germ agglutinin-sensitive ATPase in yeast nuclei

AbstractWe have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the presence of WGA-binding materials around the nuclear pore under an electron microscope. Using WGA-agarose column chromatography of yeast nuclear extracts, a novel Mg2+-dependent ATPase was isolated. Its activity was highly sensitive to WGA and stimulated by Nonidet P-40 or phosphatidylserine. We suggest that the WGA-sensitive ATPase plays a role in yeast nuclear transport of DNA

State space modeling approach to decompose daily sales of a restaurant into time-dependent multi-factors

Kyushu University 21st Century COE Program Development of Dynamic Mathematics with High Functionality九州大学21世紀COEプログラム「機能数理学の構築と展開」Daily sales of catering establishments are affected by several factors: The day of the week, national holidays, weather, events taking place near the site, etc. Therefore, it is beneficial to construct a model decomposing a time series of the sales into such factors. Thus, the model will allow us to predict future sales with good accuracy in order to plan various management strategies such as purchasing, staff assignments, new store openings, etc. In this study, we propose a prediction method for the daily sales of a catering establishment based on a state space model framework in which an optimal model is selected by an information criterion; we then apply it to 2-year daily sales data of a restaurant that is adjacent to a large-scale convention center and office buildings

Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae

The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of NPS1, nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damag

State space modeling approach to decompose daily sales of a restaurant into time-dependent multi-factors

Daily sales of catering establishments are affected by several factors: The day of the week, national holidays, weather, events taking place near the site, etc. Therefore, it is beneficial to construct a model decomposing a time series of the sales into such factors. Thus, the model will allow us to predict future sales with good accuracy in order to plan various management strategies such as purchasing, staff assignments, new store openings, etc. In this study, we propose a prediction method for the daily sales of a catering establishment based on a state space model framework in which an optimal model is selected by an information criterion; we then apply it to 2-year daily sales data of a restaurant that is adjacent to a large-scale convention center and office buildings.Kyushu University 21st Century COE Program Development of Dynamic Mathematics with High Functionality九州大学21世紀COEプログラム「機能数理学の構築と展開