大麻の鑑識と最近の乱用薬物事情

Cannabis pollens was identified using anti-cannabis pollen IgE obtained from an allergic patient against cannabis pollens. Cannabis pollens were kept on PVDF membrane and treated with sodium carbonate resulting in antigen protein fixed on membrane. The membrane was stained by the treatment with anti-cannabis pollen IgE, peroxidase labeled secondary monoclonal antibody (MAb) and finally substrate. MAb against tetrahydrocannabinolic acid (THCA) identified only cannabis plant in various plant species. Furthermore, since anti-THCA MAb had a wide cross-reactivity against all cannabinoids, all types of marihuana can be determined. We also discussed the identification methodology of marihuana using biosynthetic enzyme gene related to cannnabidiolic acid (CBDA) and THCA. Recently the circumstances surrounding illegal drugs and marihuana are changeable. Although the punishment and treatment for the suspects related illegal drugs and marihuana have been tolerant in European country, the opposite tendency has been found in Asian country. Supporting countries for the medical marihuana are increasing in like Netherland, Finland and the other European countries, Canada and a part of the United State