Protein regulation: Tag wrestling with relatives of ubiquitin

AbstractUbiquitin modification is a well established way of regulating protein levels and activities. Modification by related ubiquitin-like proteins is turning out to have a diverse range of interesting cellular functions

Applications des électrodes volumiques à divers problèmes électrochimiques

The specific surface area of several porous electrodes has been determined by an electrochemical method, which yields similar results as the pressure drop method. Modified reticulated vitreous C has been applied to the electrochemical reduction of O2 to H2O2. Detoxification of waste water containing cyanid or Ni at low concentration is described

Ultrawide bandwidth wavelength monitor based on a pair of tilted fiber Bragg gratings

A wavelength monitor based on a pair of tilted fiber Bragg gratings is reported. The monitor exhibits a subpicometer wavelength selectivity over a broad wavelength range (> 30 nm), and a low insertion loss (< 1 dB) for laser light with polarization matching the reference polarization of the device. Simulations show that a wavelength coverage up to 100 nm is possible and that the resolution degradation due to changes in the polarization state can be avoided by proper design of the gratings

Large-field high-resolution X-ray microscope for studying laser plasmas

International audienceIn 1948, P. Kirkpatrick and A. V. Baez developed an x-ray microscope (energy range about 100 eV-10 keV) composed of two concave spherical mirrors working at grazing incidence. That device, named KB microscope, presents a 3-5 μm resolution within a field having a radius about 100 μm; outside that field, its resolution lowers rapidly when the object point recedes from the center. The adjunction of two similar mirrors can notably increase the useful field (typically, the resolution can be better than 10 μm within a 2-mm-diam field of view), which is necessary for studying laser plasmas. Its main advantage with respect to more simple optics, as the pinhole, is that it can be located far enough from the plasma to avoid any destruction during the shot. We describe such a microscope that we call KBA microscope and present some images of fine metallic grids. Those grids were backlighted by x-raysources, either a cw one or a series of laser plasmas from the Octal-Héliotrope facility. Examining the films in detail shows that the experimental results are very close to the theoretical characteristics; hence the interest of this device for the x-raydiagnostics on the future powerful laser facilities

Versatile Virus-Like Particle Carrier for Epitope Based Vaccines

BACKGROUND: Recombinant proteins and in particular single domains or peptides are often poorly immunogenic unless conjugated to a carrier protein. Virus-like-particles are a very efficient means to confer high immunogenicity to antigens. We report here the development of virus-like-particles (VLPs) derived from the RNA bacteriophage AP205 for epitope-based vaccines. METHODOLOGY/PRINCIPAL FINDINGS: Peptides of angiotensin II, S.typhi outer membrane protein (D2), CXCR4 receptor, HIV1 Nef, gonadotropin releasing hormone (GnRH), Influenza A M2-protein were fused to either N- or C-terminus of AP205 coat protein. The A205-peptide fusions assembled into VLPs, and peptides displayed on the VLP were highly immunogenic in mice. GnRH fused to the C-terminus of AP205 induced a strong antibody response that inhibited GnRH function in vivo. Exposure of the M2-protein peptide at the N-terminus of AP205 resulted in a strong M2-specific antibody response upon immunization, protecting 100% of mice from a lethal influenza infection. CONCLUSIONS/SIGNIFICANCE: AP205 VLPs are therefore a very efficient and new vaccine system, suitable for complex and long epitopes, of up to at least 55 amino acid residues in length. AP205 VLPs confer a high immunogenicity to displayed epitopes, as shown by inhibition of endogenous GnRH and protective immunity against influenza infection

Switching Off your Device Does Not Protect Against Fault Attacks

Physical attacks, and among them fault injection attacks, are a significant threat to the security of embedded systems. Among the means of fault injection, laser has the significant advantage of being extremely spatially accurate. Numerous state-of-the-art studies have investigated the use of lasers to inject faults into a target at run-time. However, the high precision of laser fault injection comes with requirements on the knowledge of the implementation and exact execution time of the victim code. The main contribution of this work is the demonstration on experimental basis that it is also possible to perform laser fault injection on an unpowered device. Specifically, we targeted the Flash non-volatile memory of a 32-bit microcontroller. The advantage of this new attack path is that it does not require any synchronisation between the victim and the attacker. We provide an experimental characterization of this phenomenon with a description of the fault model from the physical level up to the software level. Finally, we applied these results to carry out a persistent fault analysis on a 128-bit AES with a particularly realistic attacker model which reinforces the interest of the PFA