3,818 research outputs found
In situ apparatus for the study of clathrate hydrates relevant to solar system bodies using synchrotron X-ray diffraction and Raman spectroscopy
Clathrate hydrates are believed to play a significant role in various solar
system environments, e.g. comets, and the surfaces and interiors of icy
satellites, however the structural factors governing their formation and
dissociation are poorly understood. We demonstrate the use of a high pressure
gas cell, combined with variable temperature cooling and time-resolved data
collection, to the in situ study of clathrate hydrates under conditions
relevant to solar system environments. Clathrates formed and processed within
the cell are monitored in situ using synchrotron X-ray powder diffraction and
Raman spectroscopy. X-ray diffraction allows the formation of clathrate
hydrates to be observed as CO2 gas is applied to ice formed within the cell.
Complete conversion is obtained by annealing at temperatures just below the ice
melting point. A subsequent rise in the quantity of clathrate is observed as
the cell is thermally cycled. Four regions between 100-5000cm-1 are present in
the Raman spectra that carry features characteristic of both ice and clathrate
formation. This novel experimental arrangement is well suited to studying
clathrate hydrates over a range of temperature (80-500K) and pressure
(1-100bar) conditions and can be used with a variety of different gases and
starting aqueous compositions. We propose the increase in clathrate formation
observed during thermal cycling may be due to the formation of a quasi
liquid-like phase that forms at temperatures below the ice melting point, but
which allows easier formation of new clathrate cages, or the retention and
delocalisation of previously formed clathrate structures, possibly as amorphous
clathrate. The structural similarities between hexagonal ice, the quasi
liquid-like phase, and crystalline CO2 hydrate mean that differences in the
Raman spectrum are subtle; however, all features out to 5000cm-1 are diagnostic
of clathrate structure.Comment: Astronomy & Astrophysics, in press. 6 page
Quinine blocks 5-HT and 5-HT3 receptor mediated peristalsis in both guinea pig and mouse ileum tissue
Introduction. Quinine is commonly used to treat malaria; however one of the principal side effects is gastrointestinal disturbances (White, 1992). 5-HT3 receptors modulate gut peristalsis (Chetty et al., 2006), and, as quinine has been shown to act as a 5-HT3 receptor antagonist (Thompson and Lummis, 2008) it is possible that these side effects result from actions at gut 5-HT3 receptors. To address this question, we examined the ability of quinine to antagonise 5-HT and 5-HT3 mediated peristalsis in guinea pig and mouse ileum.
Methods. Ileum was excised from male guinea pigs (200-300g) and C57BL/6 mice (25-35g) following cervical dislocation. Ileum segments (3-5 cm) were mounted in 50 ml organ baths containing Tryodeâs solution at 35-37 °C. Concentration-response curves were constructed for 5-HT and the selective 5-HT3 agonist 2-Me-5-HT (non-cumulative doses). Quinine was pre-applied for 10 min and inhibition measured using agonist concentrations that elicited a submaximal response.
Results. Concentration-dependent contractions produced by 5-HT (pEC50 = 5.45 ± 0.17, n = 8) and the selective 5-HT3 agonist 2-Me-5-HT (5.01 ± 0.17, n = 11) were not significantly different (Studentâs t-test, t = 0.619, df = 17, p = 0.544) in guinea pig ileum. Increasing concentrations of quinine were able to antagonise the activities of both 5-HT (pIC50 = 5.03 ± 0.2, n = 6) and 2-Me-5HT (pIC50 = 4.59 ± 0.26, n = 4). At mouse ileum, 5-HT (pEC50 = 7.57 ± 0.33, n = 9) was more potent (Studentâs t-test, t = 3.6, df = 12, p = 0.004) than 2-Me-5-HT (pEC50 = 5.45 ± 0.58, n = 5). Quinine antagonised both the 5-HT (pIC50 = 4.87 ± 0.31, n = 7) and 2-Me-5-HT-induced (pIC50 = 6.18 ± 1.14, n = 4) contractions.
Conclusions. These results support previous electrophysiological studies that identified quinine as an antagonist at recombinant 5-HT3 receptors with IC50 values comparable with those reported here (pIC50 = 4.87, Thompson et al., 2007). Further, we found that quinine completely blocked 5-HT induced contractions in mouse and guinea pig, raising the possibility that quinine targets other 5-HT receptors in the gut (e.g., 5-HT4 receptors) and may influence intestinal function
Consensual Qualitative Research: An Update
The authors reviewed the application of consensual qualitative research (CQR) in 27 studies published since the methodâs introduction to the field in 1997 by C. E. Hill, B. J. Thompson, and E. N. Williams (1997). After first describing the core components and the philosophical underpinnings of CQR, the authors examined how it has been applied in terms of the consensus process, biases, research teams, data collection, data analysis, and writing up the results and discussion sections of articles. On the basis of problems that have arisen in each of these areas, the authors made recommendations for modifications of the method. The authors concluded that CQR is a viable qualitative method and suggest several ideas for research on the method itself
Sustainability Survey report
Although the idea of sustainability means different things to different people, one of the most common definitions comes from the UNâs 1987 Brundtland Report, which states that sustainability is that which meets the needs of the present without compromising the ability of future generations to meet their own needs. In practice, sustainability means balancing environmental protection with economic vitality and social justice. Since the 1990s, sustainability has become a powerful framework for organizational transformation, particularly at institutions of higher education. Today over 1,350 colleges and universities offer degrees in sustainability and use sustainability as a guiding principle for administrative decisions across campus. On our own campus, the Environmental Studies and Sustainability major grew 144% in the past year while enrollment in other programs declined. For many people born in the early twenty-first century, sustainability is an exciting movement that seeks solutions to the daunting economic, environmental, and social problems that their generation will face. As training grounds for the future, universities like Northern have a responsibility to prepare todayâs students for these global challenges
Spatial and Temporal Patterns of Small-Scale Mixing in Drake Passage
Temperature and salinity profiles obtained with expendable CTD probes throughout Drake Passage between February 2002 and July 2005 are analyzed to estimate turbulent diapycnal eddy diffusivities to a depth of 1000 m. Diffusivity values are inferred from density/temperature inversions and internal wave vertical strain. Both methods reveal the same pattern of spatial variability across Drake Passage; diffusivity estimates from inversions exceed those from vertical strain by a factor of 3 over most of Drake Passage. The Polar Front (PF) separates two dynamically different regions. Strong thermohaline intrusions characterize profiles obtained north of the PF. South of the PF, stratification is determined largely by salinity, and temperature is typically unstably stratified between 100- and 600-m depth. In the upper 400 m, turbulent diapycnal diffusivities are O(10^(â3) m2 s^(â1)) north of the PF but decrease to O(10^(â4) m2 s^(â1)) or smaller south of the PF. Below 400 m diffusivities typically exceed 10^(â4) m^2 s^(â1). Diffusivities decay weakly with depth north of the PF, whereas diffusivities increase with depth and peak near the local temperature maximum south of the PF. The meridional pattern in near-surface mixing corresponds to local maxima and minima of both wind stress and wind stress variance. Near-surface diffusivities are also found to be larger during winter months north of the PF. Wind-driven near-inertial waves, strong mesoscale eddy activity, and double-diffusive convection are suggested as possible factors contributing to observed mixing pattern
Examining the Development of a Social Media Strategy for a National Sport Organisation A Case Study of Tennis New Zealand
The application of technology and the rise in social media has not gone unnoticed in sports and has become a vital tool for sport marketers as sport consumersâ media consumption grows. This paper offers an analysis on the development and maintenance of a social media strategy for a National Sport Organisation (NSO) in New Zealand. This initiative, implemented for Tennis New Zealand (TNZ) in 2010, was conducted to provide a minority sport (in terms of mainstream media) with its first online social media presence. This study involved a qualitative and quantitative analysis of Tennis New Zealandâs (TNZ) development and maintenance of a social media strategy through fieldwork, social media metrics data collection and analysis, and evaluation, and highlights a number of issues in the development and effective utilisation of social media for an NSO. In particular applying a âone size fits allâ approach to its management despite the uniqueness of the sport product. Creative online strategies using technologies such as Facebook must be employed, monitored and evaluated to ensure they continue to meet the needs and expectations of all stakeholders. Such strategies include the use of promotions, âbehind-the-scenesâ material, and constant engagement and conversation with fans and followers
Influence of hydroperiod on aquatic food-web structure and energy production in a floodplain wetland:implications for environmental flow management
Context: Environmental water is often used to manage floodplain wetlands that support many taxa, both terrestrial and aquatic. It is important to optimise the managed hydroperiod to maximise the provision of aquatically derived resources from wetlands. Aims: To test the hypothesis that increasing hydroperiod affects food-web structure and energy production in floodplain wetlands. Methods: Fatty acids and stable isotopes of ÎŽ13C and ÎŽ15N were used to define food-web structure, and estimate total energy production throughout a managed inundation event in a wetland in the northern Murray-Darling Basin. Key results: Food-web complexity increased with an increasing hydroperiod in line with predictable patterns of community assemblage development, before reducing sharply immediately prior to drying. Energy availability increased with an increasing hydroperiod and there was a strong correlation (Ï = 0.669, P = 0.0001) between energy availability and fatty acid concentration, which was in turn related to patterns of taxon occurrence. Conclusions and implications: Hydroperiod exerts a strong influence on aquatic invertebrate community trophic dynamics and energy provision. Planned flows should support maturation and stabilisation of the invertebrate community to optimise energy provision to consumers.</p
Are baseline ultrasound and mammographic features associated with rates of pathological completes response in patients receiving neoadjuvant chemotherapy for breast cancer?
Non-aqueous formation of the calcium carbonate polymorph vaterite: astrophysical implications
We study the formation of calcium carbonate, through the solid-gas
interaction of amorphous Ca-silicate with gaseous CO2, at elevated pressures,
and link this to the possible presence of calcium carbonate in a number of
circumstellar and planetary environments. We use in-situ synchrotron X-Ray
powder diffraction to obtain detailed structural data pertaining to the
formation of the crystalline calcium carbonate phase vaterite and its evolution
with temperature. We found that the metastable calcium carbonate phase vaterite
was formed alongside calcite, at elevated CO2 pressure, at room temperature and
subsequently remained stable over a large range of temperature and pressure. We
report the formation of the calcium carbonate mineral vaterite whilst
attempting to simulate carbonate dust grain formation in astrophysical
environments. This suggests that vaterite could be a mineral component of
carbonate dust and also presents a possible method of formation for vaterite
and its polymorphs on planetary surfaces.Comment: Astronomy and Astrophysics, in pres
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