10 research outputs found
Primary cilia (PC) in pancreatic intra-epithelial neoplasia (PanIN) and pancreatic cancer cells (PDAC).
<p><b>(A)</b> Comparison between PanIN 1a (*) and PanIN 1B (#), the latter showing papillary epithelium and reduced number of cilia. <b>(B)</b> PanIN 3 lesion. Epithelial cells do not carry cilia while PC are present in stromal cells (*). <b>(C)</b> Loss of epithelial PC in PDAC (indicated by arrows), while in the stromal cells there is a noticeable increase of both the length of PC and the number of PC carrying cells (*). <b>(D)</b> Length of epithelial PC is decreased in PanIN lesions in comparison to normal pancreas (donor). <b>(E)</b> Gradual loss of epithelial PC in PanIN lesions. In PDAC, almost no epithelial PC were detected. <b>(F)</b> In stromal tissue around PanIN lesions and PDAC (G1/G2), an increased length of PC and <b>(G)</b> increased fraction of cilia carrying cells was evident compared to normal pancreas (donor). Kruskal-Wallis test: <i>p</i> < 0.0001, post-hoc Dunns test: ***<i>p</i> < 0.001 vs. donor, **<i>p</i> < 0.01 vs donor, *<i>p</i> < 0.01 vs donor, ###<i>p</i> < 0. 001 vs PanIN 1A, ##<i>p</i> < 0. 01 vs PanIN 1A, +++<i>p</i> < 0. 001 vs PanIN 1B, +<i>p</i> < 0. 05 vs PanIN 1B, acetylated α-tubuline: red, γ-tubuline: green, DAPI: blue. Mean ± SEM.</p
Re-Distribution of primary cilia (PC) from epithelial to stromal cells in pancreatic carcinogenesis.
<p>The fraction of primary cilia (PC) carrying cells decreases in epithelia, while there is a simultaneous increase of ciliated cells in stromal tissue during pancreatic carcinogenesis (from PanIN 1A to pancreatic G1/G2 ductal adenocarcinomas (PDAC). Mean ± SEM.</p
Schematical structure and visualization of primary cilia (PC).
<p><b>(A)</b> Cross section of primary cilia, consisting of 9 microtubule duplets containing α and β tubline. <b>(B)</b> Structure of PC. Cilia are fixed by basal bodies on the cell membrane. <b>(C)</b> Immunofluorescent visualization of PC in Panc1 cells. The axonem is stained in red (acetylated α-tubline), the basal body is stained green (γ-tubline). <b>(D)</b> Immunofluorescent visualization of PC and the spindle apparatus in the BxPx3 cancer cell line.</p
PC in pancreatic stellate cells (PSC) and pancreatic cancer cell lines.
<p><b>(A)</b> MiaPaCa2 cells developing sporadic PC. <b>(B)</b> In these starving Panc1 cells, PC are expressed more frequently. <b>(C)</b> Multiple mitosis can be seen in Capan1 cells. The spindle apparatus never appears together with PC, inferring it is reabsorbed during mitosis. <b>(D)</b> PC are frequently present even in isolated PSC.</p
Primary cilia (PC) in intraductal papillary-mucinous neoplasia (IPMN).
<p><b>A/B)</b> Two benign and <b>(C)</b> one malignant IPMN without PC. <b>(D)</b> In stromal tissue surrounding IPMN lesions, an increased fraction of cilia carrying cells (<b>E</b>, Kruskal-Wallis test: <i>p</i> < 0. 01) and an increased length of PC (<b>F</b>, Kruskal-Wallis test: <i>p</i> < 0. 001) were detected compared to normal pancreas (donor). Post-hoc Dunns test: *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0. 001, acetylated α-tubuline: red, γ-tubuline: green, DAPI: blue. Mean ± SEM.</p
Hombres de Terranova: la pesca del bacalao 1926-2004
El hilo conductor del libro es el aspecto subjetivo, la experiencia
vivida de estos Hombres que participan en los hechos. Éste es, en definitiva,
el propósito de este trabajo: mostrar, siguiendo a Halbwachs, la existencia de
unas memorias colectivas que se entretejen con la historia general. El
comienzo de esta actividad industrial en 1926 obedece a la necesidad de
abastecer el mercado nacional de pescado seco, que en este caso es bacalao
salado. Los acontecimientos de la guerra civil española o la Segunda Guerra
Mundial, constituyen grandes hitos a tener en cuenta, ya que aportan
planificaciones económicas en los países donde se potencia una gran
concurrencia internacional, y, del mismo modo, el hecho de pasar de un
Mare Liberum, a un mar gestionado por los países ribereños, que extienden
su jurisdicción a doscientas millas marinas aguas adentro, determinará la
desaceleración de la expansión de la flota bacaladera. Se analizan
manipulaciones de conceptos y se reflexiona sobre la génesis y el desarrollo
de la sobrepesca, la situación interna de Terranova, como la emergencia de
sus propios símbolos, la llegada de la moratoria en 1992 y la no
recuperación de la especie del bacalao después de una moratoria vigente
durante más de una década
MOESM1 of Distinct pathophysiological cytokine profiles for discrimination between autoimmune pancreatitis, chronic pancreatitis, and pancreatic ductal adenocarcinoma
Additional file 1: Table S1. Comparison of cytokine levels in serum from: AIP-1, AIP-2, CP and PDAC patients
Translocation of Receptor Tyrosine Kinase receptor to cell-cell contacts.
<p>Immunofluorescence staining of human umbilical vein endothelial cells following incubation with recombinant human angiopoietin-1 (30min), dialysates and glucose (24h). Angiopoietin1 and bicarbonate buffered PD fluid promote translocation of Receptor Tyrosine Kinase to the vascular endothelial cadherin (VE cadherin) cell-cell contacts (orange overlap). Magnification 400x. Representative images are given.</p
Tube formation assay.
<p>Angiogenic capacity analyzed by tube formation of human endothelial cells treated with different PD fluids. Automated analysis performed with CellProfiler Software showing (A) mean area and number of closed areas by endothelial cell branches (B) and (C) illustrative example of methodology (left image: red lines mark endothelial cells and their branches; right image: areas framed by endothelial cells are shown in different colors). Representative examples of the tubular network formed with BPDF and LPDF from 4 independent experiments performed in triplicates are given in (D). *p<0.05; **p<0.01; ****p<0.0001 for BPDF vs. all other; #p<0.05 BPDF vs. LPDF.</p
Vessel morphology and angiopoietin-1 abundance in peritoneal biopsies.
<p>Angiopoietin-1 abundance per CD31 positive endothelium (A) and cross sectional vessel area (B) in the peritoneum of children on PD with bicarbonate and lactate buffered PD fluid and low glucose degradation product content (n = 8/group; Aperio<sup>®</sup> digital image analysis). Representative angiopoietin-1 staining with BPDF and LPDF are given in (C) and (D), scale bar 100μm. * p<0.05.</p