Roscovitine decreases proliferation and increases p21 with no effect on apoptosis

<p><b>Copyright information:</b></p><p>Taken from "p21 is decreased in polycystic kidney disease and leads to increased epithelial cell cycle progression: roscovitine augments p21 levels"</p><p>http://www.biomedcentral.com/1471-2369/8/12</p><p>BMC Nephrology 2007;8():12-12.</p><p>Published online 22 Aug 2007</p><p>PMCID:PMC2045080.</p><p></p> MDCK cells were seeded 24 h before treatment. Roscovitine (at the concentrations indicated) was added to the cells for 24 h and then the cells were harvested and subjected to the MTT assay as described in Materials and Methods. QM is serum-free media; * p < 0.05 compared to serum alone. Experiment shown is representative of at least three experiments

Representative immunohistochemical expression of p21 in male cystic and noncystic Han:SPRD rats (left), and male PKD and control humans (right)

<p><b>Copyright information:</b></p><p>Taken from "p21 is decreased in polycystic kidney disease and leads to increased epithelial cell cycle progression: roscovitine augments p21 levels"</p><p>http://www.biomedcentral.com/1471-2369/8/12</p><p>BMC Nephrology 2007;8():12-12.</p><p>Published online 22 Aug 2007</p><p>PMCID:PMC2045080.</p><p></p> Upper panels show p21 localization from control sections, and the lower panels show cystic sections. Also shown are sections (NI) pre-incubated with non-immune serum that demonstrate antibody specificity. In sections from control male kidneys, p21 was widely distributed, whereas in sections from cystic male kidneys, p21 was only sparsely detected