Letter, 1913, May 27, Harriet Taylor Upton to Dear Club President [Martha McClellan Brown]

A letter from Harriet Taylor Upton of the Ohio Woman Suffrage Association regarding sending an annual report from the association and requesting that local clubs get together to read the report.https://corescholar.libraries.wright.edu/special_ms147_correspondence/1026/thumbnail.jp

The Synthesis of 2-Carboxyethyl-P, P-Diphenylphosphine and a Study of it as a Potential Chelating Agent

This 24 page thesis examines the synthesis of organo-phosphorous acids and their stability as ligands

Letter, 1915, July 21, Harriet Taylor Upton to Mrs. M. McClellan Brown [Martha McClellan Brown]

A letter from Harriet Taylor Upton of the Ohio Woman Suffrage Association to Martha McClellan Brown indicating her disappointment for Brown not coming to Springfield, the status of a mutual acquaintance, and more.https://corescholar.libraries.wright.edu/special_ms147_correspondence/1044/thumbnail.jp

Letter, 1913, February 17, Harriet Taylor Upton to Mrs. M. McClellan Brown [Martha McClellan Brown]

A letter from Harriet Taylor Upton of the Ohio Woman Suffrage Association to Martha McClellan Brown regarding organizing transportation via train for 500 marchers.https://corescholar.libraries.wright.edu/special_ms147_correspondence/1019/thumbnail.jp

Letter, 1913, February 26, Harriet Taylor Upton to Dear Club President [Martha McClellan Brown]

A letter from Harriet Taylor Upton of the Ohio Woman Suffrage Association asking for a status update about the Plan of Work adopted at the Columbus Convention. She also writes that Carrie Chapman Catt will be the featured speaker for their upcoming conferences.https://corescholar.libraries.wright.edu/special_ms147_correspondence/1023/thumbnail.jp

Harriet Taylor Upton Study Club

A directory of officers, committees, and members of the Harriet Taylor Upton Study Club and the schedule of club meetings for September 1913 through May 1914. The club’s constitution is included in the document. Martha McClellan Brown served as president.https://corescholar.libraries.wright.edu/special_ms147_ephemera/1006/thumbnail.jp

Genetic and morphological differentiation in Populus nigra L.:isolation by colonization or isolation by adaptation?

Identifying processes underlying the genetic and morphological differences among populations is a central question of evolutionary biology. Forest trees typically contain high levels of neutral genetic variation, and genetic differences are often correlated with geographic distance between populations [isolation by distance (IBD)] or are due to historic vicariance events [isolation by colonization (IBC)]. In contrast, morphological differences are largely due to local adaptation. Here, we examined genetic (microsatellite) and morphological (from a common garden experiment) variation in Populus nigra L., European black poplar, collected from 13 sites across western Europe and grown in a common garden in Belgium. Significant genetic differentiation was observed, with populations from France displaying greater admixture than the distinct Spanish and central European gene pools, consistent with previously described glacial refugia (IBC). Many quantitative traits displayed a bimodal distribution, approximately corresponding to small-leaf and large-leaf ecotypes. Examination of nine climatic variables revealed the sampling locations to have diverse climates, and although the correlation between morphological and climatic differences was significant, the pattern was not consistent with strict local adaptation. Partial Mantel tests based on multivariate summary statistics identified significant residual correlation in comparisons of small-leaf to large-leaf ecotypes, and within the small-leaf samples, but not within large-leaf ecotypes, indicating that variation within the small-leaf morphotype in particular may be adaptive. Some small-leaf populations experience climates very similar to those in large-leaf sites. We conclude that adaptive differentiation and persistent IBC acted in combination to produce the genetic and morphological patterns observed in P. nigra

Functional analysis of zebrafish innate immune responses to inflammatory signals

Injury, infection and tissue malfunction are triggers of inflammation which if not regulated may acquire new characteristics that result in pathological outcomes. Since innate immunity plays a key role in the resolution of acute inflammation knowledge of the regulation of this component of the host response is relevant to understanding processes in disease progression and therefore has potential clinical benefits. In this thesis I have applied zebrafish as a model organism to investigate the response of innate immune cells to qualitatively distinct inflammatory signals in the absence of adaptive immunity. Using a zebrafish embryo wound injury model I have investigated leukocyte migration profiles by in vivo imaging. In response to wound alone leukocytes migrated to the site of injury with predominantly random walk behaviour. However, the addition of lipopolysaccharide (LPS) enhanced recruitment and influenced the directionality of leukocyte migration to the wound. I demonstrate that leukocyte dynamic behaviour is also dependent on the location of the cells. The LPS enhanced directionality and reduced the random walk behaviour of the leukocytes, and these effects were ablated in the presence of the p38 mitogenactivated protein kinase (MAPK) specific inhibitor SB203580. Cytokine gene profiling in adult zebrafish leukocytes reveals that LPS can stimulate a pro-inflammatory response via the activation of p38 MAPK characteristic of mammalian innate immune responses. It is documented in mammalian innate immune cells that LPS can modulate Notch mediated signalling and thereby cell function. Using zebrafish with null mutations in Notch, which provide an unbiased in vivo model, I have investigated the influence of Notch signalling on leukocyte recruitment and demonstrate that migration to a wound injury is reduced. However, this effect is due to decreased cell numbers and not altered function as the Notch signalling inhibitor DAPT had no effect of recruitment to wound injury. The defect in myelomonocyte numbers was also present in adult zebrafish and this was partially compensated for by an increase in lymphocytes. The experimental results that I report here highlight zebrafish as a model 2 organism for studying the function and regulation of innate immunity. The unique optical translucency, which permits in vivo imaging of host responses in real-time, facilitates the analysis of the innate immune response to different inflammatory signals and immune modulators