HUBUNGAN KADAR GLUKOSA DARAH DENGAN PERTUMBUHAN A. actinomycetemcomitans PADA TIKUS DIABETES MELITUS

Diabetes mellitus is a systemic disease characterized by insulin deficiency. Common symptoms in people with diabetes is elevated blood glucose is called hyperglycemia. Manifestations in the oral cavity are inflammation of gingival tissue or periodontitis. The bacteria that play a role in the severity of periodontal disease is A. actinomycetemcomitans. The purpose of this study was to determine the relationship of blood glucose levels with the growth of A. actinomycetemcomitans in DM rats treated with insulin therapy. Methode 20 three month old male Wistar rats with weight of 170-200 grams were divided into two groups. First, periodontitis and DM were manipulated into all groups through aloksan injection with dose of 170 mg/kg. Those rats in group I were treated with insulin, those in group II were without treatment. In the third and seventh weeks, the blood glucose levels and the number of A. actinomycetemcomitans was measured. The data was tested by using Pearson test. The results showed that there is a strong relationship between blood glucose levels with the growth of A. actinomycetemcomitans. Conclusion: The increasing blood glucose levels are very strongly associated with an increase in the number of A. actinomycetemcomitans and otherwise in DM rats with insulin therapy

PERIODONTITIS DAN DIABETES MELITUS

Periodontitis is a chronic infection disease caused by periodontopathogen bacteria in the oral cavity. Severity of periodontitis can be affected by systemic diseases such as diabetes mellitus. Diabetes mellitus is occurred metabolic disruption that is characterized by hyperglycemia due to insulin deficiency. Increasing blood glucose levels can lead to manifestations in the oral cavity in the form of inflammation of the gingival tissue, attachment loss and alveolar bone destruction. Periodontitis is one of the sixth complications of diabetes mellitus. There are inter- related relationship between periodontitis and diabetes mellitus

PROFIL KEBERSIHAN DAN PERILAKU MENJAGA KESEHATAN GIGI DAN MULUT PADA LANSIA DI DESA DARSONO KABUPATEN JEMBER

Pendahuluan: Pembangunan kesehatan di Indonesia dititik-beratkan untukmeningkatkan kesehatan masyarakat yang berkualitas secara menyeluruh, terpadu, danberkelanjutantermasuk didalamnya kesehatan gigi dan mulut lanjut usia (lansia).Kebersihan gigi dan mulut merupakan masalah yang sangat perlu diperhatikan pada lanjutusia, karena dapat memicu timbulnya berbagai penyakit di rongga mulut.Tujuan Penelitian: Untuk mengetahui profil kesehatan gigi dan mulut lansia denganmelihatstatus kebersihan mulut oral hygiene indeks simplified (OHI-S) didesa DarsonoKecamatan Arjasa Kabupaten Jember.Metode Penelitian: Jenis penelitian ini adalah diskriptif observasional secara crosssectional yang dilakukan pada lansia bulan september 2016. Lansia yang yang terlibatberjumlah 44 orang meliputi laki-laki dan perempuan. Pemeriksaan yang dilakukan  adalah skor debris dan kalkukus menggunakan Oral Hygiene Index Simplified (OHI-S)Green and Vermillion, 1964Hasil Penelitian: menunjukkan pra lansia yang memiliki OHI-S baik hanya 2 orang (10%dari total lansia yang dilakukan pemeriksaan), dan pada kelompok lansia skor OHI-S yangbaik hanya berjumlah 2 orang (10,5 %), sisanya memiliki OHI-S buruk. Kondisi kebersihanmulut lansia di Desa Darsono Kecamatan Arjasa Kabupaten Jember dalam kondisi buruksehingga perlu dilakukan peningkatan kesehatan gigi dan mulutnya yang lebih intensive. Kata Kunci : Kesehatan gigi mulut, Lansia, Indeks OHI-S, Desa Darsono, KecamatanArjasa Jember &nbsp

Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells

Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil  (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels

Daya Hambat Ekstrak Biji Kopi Robusta (Coffea canephora) terhadap Pertumbuhan Fusobacterium nucleatum

Fusobacterium nucleatum is a bacteria found in normal flora of the oral cavity that plays a role in the occurrence of periodontal disease. The prevalence of periodontal disease in Indonesia reaches 70%. One treatment by using medicinal plants is robusta coffee beans. Robusta coffee beans contain volatile acids, chlorogenic acids, caffeine acids, phenols and caffeine suspected to be antibacterial. The aim was to determine the inhibition of coffee robusta extract on growth of Fusobacterium nucleatum. This study used disc diffusion method with 4 samples in each study group. The study group consisted of 6 treatment groups (12,5%, 25%, 50% and 100% coffee robusta extract), positive control group (chlorhexidine gluconate 0,2%), and negative control group (sterile aquades). Data were analyzed using One Way Anova test and LSD (Least Significant Difference) test. Coffee robusta extract has the ability to inhibit the growth of Fusobacterium nucleatum. The concentration of coffe robusta extract 100%, 50%, 25%, 12,5% have same ability to inhibit the growth of Fusobacterium nucleatum

Daya Antibakteri Ekstrak Kulit Apel Manalagi (Malus sylvestris Mill.) Terhadap Pertumbuhan Streptococcus mutans (Antibacterial Activity of Manalagi Apple Peel (Malus sylvestris Mill.) Extract on The Growth of Streptococcus mutans)

Apple is a popular fruit which is consumed by many people in Indonesia. Manalagi apple's peel contains polyphenols that have antibacterial effects. Streptococcus mutans is the normal flora that can cause oral disease. One of alternative medications to prevent the disease by using natural products as antibacterial. This research is aimed to determine the antibacterial activity manalagi apple's peel extract on the growth of S. mutans and minimum concentration to inhibit growth. The method of this research is well diffusion method with 8 samples for each treatment. Samples consisted of five treatment groups which is manalagi apple's peel extract with concentration of 100%, 50%, 25%, positive control group (chlorhexidine 0.2%), and negative control group (aquadest sterile). Data were analyzed using the Kruskal-Wallis and Mann-Whitney test. The results of this research showed that manalagi apple's peel extract at all concentrations have antibacterial activity against the growth of S.mutans. Minimum inhibition concentration of manalagi apple's peel extract is 25%. The conclusion of this research proves that manalagi apple's peel extract has antibacterial activity against S.mutans growth.   Keywords: Antibacterial activity, manalagi apple's peels, Streptococcus mutans, well difussion method

STATUS KEBERSIHAN MULUT DAN KESEHATAN PERIODONTAL PASIEN YANG DATANG KE KLINIK PERIODONSIA RSGM UNIVERSITAS JEMBER PERIODE AGUSTUS 2009 – AGUSTUS 2010

Level of oral hygiene have a close relationship with periodontal health status; where good oral hygiene will minimize the occurrence of periodontal disease. Aims of this study were to assess the oral hygiene status and periodontal health patients that come to Department of Periodontics of Jember University Dental Hospital at August 2009 until August 2010. This type of research is an observational study of clinical (cross sectional). A total of 155 sample (87 males and 66 females) were examined by using Oral Hygiene Index-Simplified (OHI-S) and Periodontal Index. Good oral hygiene was seen in 11% of total sample examined, 54,2% had fair oral hygiene and rest 34,8% showed poor oral hygiene. 0,6% of total sample examined had healthy periodontal, 18,1% had simple gingivitis, 41,3% had initial of periodontal disease destructive, 37,45 had periodontal disease destructive and 2,6% had terminal disease. Oral hygiene status and periodontal health examination revealed that majority of sample had fair oral hygiene and periodontal disease destructive

EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN POST-GINGIVECTOMY WOUND HEALING

Background: Gingivectomy is a periodontal surgical procedure that eliminates excess gingival tissues by excision, usually to remove a gingival pocket which leads to scars and recontours the gingival to its proper anatomical form. Wound healing is a process to restore the missing functions and structures of a tissue due to injuries or inflammations. Robusta coffee bean contains many bioactive compounds that can accelerate the wound healing process. The study aimed to determinate the effectiveness of robusta coffee bean extract gel with concentration 40%,50%,60% on collagen fibers density in post-gingivectomy wound healing Method: 36 Wistar rats were divided into 9 groups: control, gingivectomy without therapy, gingivectomy +40% robusta coffee bean extract gel, gingivectomy +50% robusta coffee bean extract gel, and gingivectomy+60% robusta coffee bean extract gel. The last 4 groups were divided again into day 3 and 7 euthanasia. The results were processed by Trichrome Mallory to visualized the collagen fibers and then measured by ImageJ software.Result: Robusta coffee beans extract gel therapy with concentration 40%, 50% and 60% had higher average collagen fibers density compared to group without therapy.Conclusion: Robusta coffee bean extract gel increased the density of collagen fibers in post-gingivectomy wound healin

Microbiological Profile in Oral Cavity Infection in Diabetic Rats with Periodontitis

Background: Diabetes melitus is a chronic metabolic disease due to the inability of the pancreas to produce insulin or the body cannot use insulin effectively.Periodontitis is one of the complications of microvascular disorders that ranks second in oral cavity diseases. One of the aerobic bacteria thought to play a role in the severity of diabetes melitus is Staphylococcus aureus, while the pathogenic anaerobic bacteria in periodontal disease are Treponema, Bacteroides, Porphyromonas, Prevotella, Capnocytophaga, Peptostreptococcus, Fusobacterium, Actinobacillus, and Eikenella. The study aimed to determine the microbiological profile in oral cavity infections of rats with diabetes melitus and periodontitisMethod: This study was experimental laboratories on male wistar rats induced by diabetes melitus and periodontitis using wire ligature on mandibular molars, which were divided into 3 treatment groups: group I (control), group II (diabetes melitus) and group III (diabetes melitus and periodontitis). Periodontitis (wire) rats were treated for 7 days and saliva was collected to identify the microbiological profile of the oral cavity. Result: Identification indicated the presence of genus and species of bacteria in the saliva of rats in 3 groups, gram positive: Staphylococcus intermedius, Staphylococcus cohnii subsp.urelyticus. Gram negative: Chromobacterium violaceum, Kleibseilla pneumoniae, Eikenella corrodens, Enterobacter sakazaki and Chryseobacterium meningosepticum. Conclusion: The number of bacterial colonies in the treatment group of diabetes melitus and periodontitis rats was greater than the treatment group of diabetes melitus rats and the control group. Gram staining results found groups of gram positive and gram negative bacteria.

STATUS KESEHATAN PERIODONTAL DAN TINGKAT KEBUTUHAN PERAWATAN PASIEN YANG DATANG KE KLINIK PERIODONSIA RSGM UNIVERSITAS JEMBER TAHUN 2011

The CPITN is a screening procedure for identifying actual and potential problems posed by periodontal disease. The aim of this study was to know periodontal status and periodontal treatment needs from patient that come to RSGM department periodontia faculty of dentistry Jember University in the year 2011. The study population comprised 191 adolescences (10 – 59 years). An observational study was conducted at RSGM of Jember University. The results of the study showed that individuals with a health periodontium is 1.05 %, score (1) with bleeding on probing is 3.14 %. Periodontal pocket (4-5mm) was recorded 17.27 %. The highest periodontal score is scores 2 (calculus) 78.53% of the population need dental scaling. We conclude that prevalence periodontal disease in patients that come to periodontics department is very high and periodontal treatment need with scaling