High-Throughput Screening of Acyl-CoA Thioesterase I Mutants Using a Fluid Array Platform

Screening target microorganisms from a mutated recombinant library plays a crucial role in advancing synthetic biology and metabolic engineering. However, conventional screening tools have several limitations regarding throughput, cost, and labor. Here, we used the fluid array platform to conduct high-throughput screening (HTS) that identified Escherichia coli ???TesA thioesterase mutants producing elevated yields of free fatty acids (FFAs) from a large (106) mutant library. A growth-based screening method using a TetA-RFP fusion sensing mechanism and a reporter-based screening method using high-level FFA producing mutants were employed to identify these mutants via HTS. The platform was able to cover >95% of the mutation library, and it screened target cells from many arrays of the fluid array platform so that a post-analysis could be conducted by gas chromatography. The ???TesA mutation of each isolated mutant showing improved FFA production in E. coli was characterized, and its enhanced FFA production capability was confirmed

Prediction of abundance of arthropods according to climate change scenario RCP 4.5 and 8.5 in South Korea

AbstractAbundance and diversity of arthropods were projected according to climate warming in South Korea. The taxa highly linked with temperature were selected for the projection. The values of abundance and richness were estimated using the mean values of abundance and richness in each temperature range. Temperature changes were based on the RCP (Representative Concentration Pathway) 4.5 and RCP 8.5, and the abundance and richness during two periods (2011 -2015, 2056 -2065) were projected. From these projected results, change of other common taxa (> 1% occurrence) were qualitatively predicted (i.e., decrease or increase). The projections showed that 45 of a total of 73 taxa will increase, 6 will change a little and 24 will decrease: the number of taxa that were expected to increase was two times more than the number of taxa that were expected to decrease. However, the overall abundance and diversity of arthropods were expected to decline as the temperature rises

Combined Fluoroscopy- and CT-Guided Transthoracic Needle Biopsy Using a C-Arm Cone-Beam CT System: Comparison with Fluoroscopy-Guided Biopsy

Abstract. We present a novel method for blind separation of instruments in polyphonic music based on a non-negative matrix factor 2-D deconvolution algorithm. Using a model which is convolutive in both time and frequency we factorize a spectrogram representation of music into components corresponding to individual instruments. Based on this factorization we separate the instruments using spectrogram masking. The proposed algorithm has applications in computational auditory scene analysis, music information retrieval, and automatic music transcription.

Method and and apparatus for processing a signal

A method of processing a signal is disclosed. The present invention includes receiving (a) downmix signal being generated from plural-channel signal and (b) spatial information indicating attribute of the plural-channel signal in order to upmix the downmix signal and including phase shift flag indicating whether phase of a frame of at least one channel of the plural-channel signal is shifted; obtaining inter-channel phase difference (IPD) coding flag indicating whether IPD value is used to the spatial information from a header of the spatial information; obtaining IPD mode flag indicating whether the IPD value is used to frame of the spatial information from the frame based on the IPD coding flag; obtaining the IPD value of parameter band in the frame, based on the IPD mode flag; upmixing plural-channel signal by applying the IPD value to the downmix signal; and shifting the phase of the frame of the at least one channel of the plural-channel signal based on the phase shift flag

Method and an apparatus for processing a signal

A method of processing a signal is disclosed. The present invention includes receiving a maximum number of band and a code value of at least one section length, calculating a bit number corresponding to the code value of the at least one section length using the maximum number of the band, and obtaining the section length information by decoding the code value of the section length based on the bit number. A method of processing a signal is disclosed. The present invention includes receiving factor information of a current frame, receiving flag information indicating whether a coding mode of the factor information is an absolute value mode or a relative value mode, and obtaining factor data of the current frame using factor data of a previous frame and the received factor information based on the flag information

Application of Virtual and Augmented Reality to the Field of Adult Education

This research reviews the extant literature and web resources on Virtual Reality (VR) and Augmented Reality (AR) to provide new insights and future direction of adult learning. Based on the review, we discuss implications for research and practice on the use of VR and AR in the field of Adult Education

Methods and apparatuses for encoding and decoding object-based audio signals

Provided are an audio encoding method and apparatus and an audio decoding method and apparatus in which audio signals can be encoded or decoded so that sound images can be localized at any desired position for each object audio signal. The audio decoding method generating a third downmix signal by combining a first downmix signal extracted from a first audio signal and a second downmix signal extracted from a second audio signal; generating third object-based side information by combining first object-based side information extracted from the first audio signal and second object-based side information extracted from the second audio signal; converting the third object-based side information into channel-based side information; and generating a multi-channel audio signal using the third downmix signal and the channel-based side information

To the Edge of M87 and Beyond: Spectroscopy of Intracluster Globular Clusters and Ultra Compact Dwarfs in the Virgo Cluster

We present the results from a wide-field spectroscopic survey of globular clusters (GCs) in the Virgo Cluster. We obtain spectra for 201 GCs and 55 ultracompact dwarfs (UCDs) using the Hectospec on the Multiple Mirror Telescope, and derive their radial velocities. We identify 46 genuine intracluster GCs (IGCs), not associated with any Virgo galaxies, using the 3D GMM test on the spatial and radial velocity distribution.They are located at the projected distance 200 kpc $\lesssim$ R $\lesssim$ 500 kpc from the center of M87. The radial velocity distribution of these IGCs shows two peaks, one at $v_{\rm r}$ = 1023 km s$^{-1}$ associated with the Virgo main body, and another at $v_{\rm r}$ = 36 km s$^{-1}$ associated with the infalling structure. The velocity dispersion of the IGCs in the Virgo main body is $\sigma_{\rm{GC}} \sim$ 314 km s$^{-1}$, which is smoothly connected to the velocity dispersion profile of M87 GCs, but much lower than that of dwarf galaxies in the same survey field, $\sigma_{\rm{dwarf}} \sim$ 608 km s$^{-1}$. The UCDs are more centrally concentrated on massive galaxies, M87, M86, and M84. The radial velocity dispersion of the UCD system is much smaller than that of dwarf galaxies. Our results confirm the large-scale distribution of Virgo IGCs indicated by previous photometric surveys. The color distribution of the confirmed IGCs shows a bimodality similar to that of M87 GCs. This indicates that most IGCs are stripped off from dwarf galaxies and some from massive galaxies in the Virgo.Comment: 19 pages, 20 figures, 8 tables, accepted for publication in Ap