Sperm characteristics and motility in Pangasianodon hypophthalmus (Sauvage, 1878) and Pangasius djambal Bleeker, 1846 (Pangasiidae, Siluriformes)

A drop in osmotic pressure appeared as the main trigger for initiating spermatozoa motility in the two catfish species studied. Motility duration was particularly short in both species (20 to 30 s). Sperm preserved at 4 degrees C in a saline medium (NaCl 155-175 mM, pH 7.5-8.5) Survived longer than in any of the other media tested

Milt-Egg Ratio in Artificial Fertilization of Pangasiid Catfish Injected by Gonadotropin Releasing Hormone-Analog (GnRH-a) and Domperidone Mixture

Study on the level of gonadotropin hormone treatments combined with latency time to induce ovulation in Pangasius djambal was conducted in the Research Instalation of Germ Plasm, Cijeruk, Bogor. This study was carried out to investigate the effect of GnRH-a and domperidone mixture on the milt production and fertilization rates of Pangasius djambal, and to determine the optimal milt-egg ratio required for artificial fertilization. Different doses of hormone i.e: 0,3; 0,5 and 0,7 ml kg"1 body weight combined with latency time of 12, 24 and 48 h after inducing hormone were applied to increase milt-production. Milt dilution was 10"1, 10~2, 10"3, 10"4, 10"5, 10"6 and 10"7and evaluated for hatching rate and normality of larvae. The results showed that mean milt production was 4,3 ml/kg body weight, and there was interaction between hormone dose of 0,5 ml/kg of body weight and latency time 12 and 24 h that giving hatching rate of 77 to 83% (

Aspek Ekologi Ikan Kancera (Tor Soro) Kuningan Dan Pematangan Gonad Melalui Implantasi Hormon Gonadotropin (HCG)

Ecological Aspect and Gonadal Maturation of Tor soro by HCG peletted Implantation. Theexperiment was conducted to determine optimal dosage of Gnrh-a hormon at gonadal maturationof mahseer from Kuningan (Tor soro). The fish was reared in concrete tanks (60m2). Thehormon was used for implantation is HCG (pregnyl). The different dosage of hormon are 250Iu/Kg-1 ; 500 Iu/Kg-1 ; 750 Iu/Kg-1 . The result of this reaserch showed that egg development isMei-Juni (average of egg diameter is 1,4 mm) and Januari (average of egg diameter is 1,35 mm),Other month, the egg diameter was not develop or atresia. Treatment with 500 Iu/Kg-1 dossageshowed the best effect on oosit diameter was at 21 day and 63 day from first oosit diameter of0,9 mm to 1,4 mm