6 research outputs found

    Konteksty prawa i praw człowieka

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    Praca recenzowana / Peer-reviewed paperW monografii i znalazły się artykuły prezentujące stan prawny na 2010 rok, pozornie ze sobą niezwiązane, bardzo zróżnicowane pod względem tematycznym, prezentujące zainteresowania badawcze osób o różnym doświadczeniu i stażu pracy. Ideą przewodnią łączącą te opracowania są prawa człowieka i ich ochrona, w szczególności w świetle uregulowań Konwencji o ochronie praw człowieka i podstawowych wolności i orzecznictwa ETPCz

    Comparative Laser Spectroscopy Diagnostics for Ancient Metallic Artefacts Exposed to Environmental Pollution

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    Metal artworks are subjected to corrosion and oxidation processes due to reactive agents present in the air, water and in the ground that these objects have been in contact with for hundreds of years. This is the case for archaeological metals that are recovered from excavation sites, as well as artefacts exposed to polluted air. Stabilization of the conservation state of these objects needs precise diagnostics of the accrued surface layers and identification of original, historical materials before further protective treatments, including safe laser cleaning of unwanted layers. This paper presents analyses of the chemical composition and stratigraphy of corrosion products with the use of laser induced breakdown spectroscopy (LIBS) and Raman spectroscopy. The discussion of the results is supported by material studies (SEM-EDS, XRF, ion-analyses). The tests were performed on several samples taken from original objects, including copper roofing from Wilanów Palace in Warsaw and Karol Poznański Palace in ŁódŸ, bronze decorative figures from the Wilanów Palace gardens, and four archaeological examples of old jewellery (different copper alloys). Work has been performed as a part of the MATLAS project in the frames of EEA and Norway Grants (www.matlas.eu) and the results enable the comparison of the methodology and to elaborate the joint diagnostic procedures of the three project partner independent laboratories

    Optimization of the culture condition of the IPEC-J2 cells line inducing morphological and functional differentiation

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    Linia komórkowa IPEC-J2 to komórki pochodzące z fizjologicznej tkanki świńskiego nabłonka jelita czczego. Poprzez optymalizację warunków hodowli tej linii komórkowej starano się otrzymać model in vitro tkanki nabłonkowej jelita, który byłby podobny morfologicznie i funkcjonalnie do tkanki, a więc umożliwiał badanie mechanizmów infekcji wirusowych w warunkach laboratoryjnych. Stopień zróżnicowania komórek badano poprzez barwienie immunofluorescencyjne preparatów komórkowych przeciwciałami skierowanymi na wilinę – białko wchodzące w skład cytoszkieletu mikrokosmków. Wykazano, że poprzez modyfikacje pożywki hodowlanej oraz prowadzenie hodowli na półprzepuszczalnej membranie pozwalającej komórkom na pobieranie składników odżywczych zarówno od strony apikalnej, jak i bazalnej uzyskano hodowle o znacząco wyższym poziomie ekspresji wiliny.IPEC-J2 cell line originate from cells of swine epithelial tissue of the jejunum. By optimizing culture conditions of IPEC-J2 cell line it was sought to develop in vitro model of the epithelial intestinal tissue, which would be morphologically and functionally similar to the tissue in order to make it possible to study mechanisms of viral infections in the laboratory conditions. The differentiation of cells was examined by immunofluorescent staining of cells preparations with antibodies directed to villin – a protein included in the microvilli cytoskeleton. By the modification of culture medium and culturing cells on the semipermeable membrane, which let cells to gather nutrients from apical and basolateral surface – the culture with significantly higher level of villin expression was obtained. The modification of culture medium and culturing cells on the semipermeable membrane lets cells to gather nutrients from apical and basolateral surface – which as a result brings to significantly higher level of villin expression in the culture

    Exposure to chemical pollutants and biological aerosol in indoor facilities for recreational and sport horses

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    Abstract Background Due to the increasing prevalence of equine non-infectious respiratory disease, the air contamination in equine housing (Stables A-C) and training facilities (indoor riding arenas A - C) was investigated. The aim of the study was to monitor gaseous pollutants, bioaerosols, and dust concentrations at three different sites (stables and riding halls), where different floor materials were used in the riding halls. Materials and methods Air quality was monitored in housing for horses and in riding halls in terms of dust concentration, the presence of gaseous chemical pollutants, and concentrations of biological aerosol. Statistical analysis was performed using analysis of variance (ANOVA). The levels obtained were compared with acceptable limits. Results Among the gaseous pollutants identified, the highest concentration was obtained for ammonia in stables B and C (16.37 and 22.39 mg/m3, respectively). Standards for total dust were exceeded in stables B and C and in riding halls B and C. The highest numbers of bacteria and fungi were recorded in stables A and C and in riding hall B. Ulocladium sp. had the highest percentage share among the moulds identified. Conclusions The results confirm that the wrong choice of bedding in the stable and indoor riding arenas may contribute, even in short training periods, to equine non-infectious respiratory disease (equine asthma). Bioaerosol suspended in the air together with released gaseous pollutants can exacerbate this phenomenon, which even in the case of short training periods can lead to equine asthma of varying degrees of severity. For this reason, the choice of floor material in riding halls should be treated as a priority, as the wrong decision can shorten the period during which the horse can be used for recreational purposes
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