Vegetative propagation of Freesia through the isolation of shoots in vitro.

Shoots from the cvs Ballerina, Aurora, Rijnveld's Golden Yellow and Rose Marie, originating from excised flower-buds, were grown in vitro and were induced to form shoots on a modified Murashige and Skoog medium containing PBA and IAA. The greatest number of new shoots/excised shoot was obtained when the medium contained PBA at 5 mg/l and IAA at 0.1 mg/l. The number of shoots formed depended on the cv. tested. Subcultured shoots, grown on a medium with IAA but without PBA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission

Freesia plantlets from flower-buds cultivated in vitro.

Flower-buds of 10 freesia cvs were grown in vitro and were induced to regenerate adventitious buds on a modified Murashige and Skoog's medium containing PBA and IAA. The formation of adventitious buds was strongly enhanced by growing the explants first in darkness and subsequently in light. Subcultured shoots, grown on a medium with IAA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission

Vegetative propagation of Nidularium fulgens Lem. in vitro

Shoot tips were isolated and placed on solid agar media (half strength Murashige and Skoog macroelements, 3% sucrose, vitamins, 1 mg l-1 BA and 0.1 mg l-1 NAA). After one month, shoots which had begun to grow were transferred to liquid media (composition as above but with no agar and BA reduced to 0.3 mg l-1). After 12 weeks about 5 axillary shoots per explant were produced. Subsequent planting of these shoots in soil achieved almost 100% rooting. (Abstract retrieved from CAB Abstracts by CABI’s permission

Yucca flaccida in kweekbuizen vegetatief te vermeerderen.

Uit onderzoek blijkt dat Yucca flaccida in vitro zeer goed is te vermeerderen via zijscheutvorming. De beworteling en de overgang naar grond leveren geen problemen o

Adventitious plantlet regeneration from floral stem explants of Nerine bowdinii W. Watts.

De adventieve voortplanting van bloemstengels van het bolgewas Nerine bowdenii W.Watts. middels weefselcultuu

Vegetatieve vermeerdering van Nidularium in vloeibare media

Het snel en veilig opbouwen van Nudilarium blijkt niet eenvoudig te zijn (dikwijls ontstaat callus waaruit adventieve scheuten ontstaan). Nidularium fulgens, die tot de Nudilariumceae behoort, kan nu in vitro gekloond worden zonder dat mutaties optreden, zodat deze soort op grote schaal veilig in de handel gebracht kan worde

Vegetatieve vermeerdering van de bonte yucca in kweekbuizen

Omdat import van bonte Yuccastammen onmogelijk is, is er een kweekbuizenmethode ontwikkeld om dit gewas in Nederland vegetatief te vermeerderen. Deze methode wordt uiteengeze

Adventitious plantlet regeneration from floral stem explants of Nerine bowdinii W. Watts.

De adventieve voortplanting van bloemstengels van het bolgewas Nerine bowdenii W.Watts. middels weefselcultuu