6 research outputs found
Covid-19 pandemi döneminde biyosensörler ve pazarlama iletişim aracı olarak gazeteler: Nevşehir örneği
1 Aralık 2019 tarihinde Çin'in Hubei bölgesinin başkenti olan Vuhan'da ortaya çıkan ve Türkiye’de ilk vakası 11 Mart 2020 tarihinde görülen ve insanoğlunun endişe, merak ve korku duymasına neden olan bu durumun sebebine SARS-COV-19 ismi verilmiştir. Aynı zamanda bu süreç Dünya Sağlık Örgütü tarafından küresel etkisi nedeniyle PANDEMİ olarak ilan edilmiştir. Belirsizlik, korku ve endişenin hakim olduğu bu süreçte hayatın idamesi için insanlığın birtakım önlemler alması gerekmektedir. Bilgi verme, eğitim, güdüleme gibi birçok farklı işlevi yerine getiren kitle iletişim araçlarında yer verilen medya içerikleri müşterilerle iletişimin doğru bir şekilde yürütülmesi açısından önem taşımaktadır. Müşterilerle iletişim sağlanan kitle iletişim araçlarından biri de gazetelerdir. Bu çalışmanın amacı, Nevşehir'de faaliyet gösteren yerel gazetelerde Covid-19 pandemi döneminde pandemi haberlerinin nasıl sunulduğunu ve okuyucuya nasıl aktarıldığını analiz etmektir. Çalışma 11 Mart 2020-10 Nisan 2020 tarihleri arasında Covid-19 ile ilgili yapılan haberleri kapsamaktadır. Yöntem olarak içerik analizi kullanılan çalışmada, haberler, konu, başlık ve kapsam olarak kategorilere ayrılarak değerlendirilmiştir
Genetic detection in simulated-saliva using MPS coated QCM sensor without DNA isolation and amplification
Early diagnosis plays a crucial role in curing the diseases. Especially, nowadays, PCR (Polymerase Chain
Reaction) is used as a Gold Standart for genetically diagnosing illnesses. Alternative ways are being searched
to be used instead of PCR because it takes more time to process, needs more investment, requires fully-armed
laboratory condition which is available and accessible only in developed-countries. An alternative method
which is able to make genetic detection in saliva would have relatively lower infection risks, and patient
comfort would be much higher by the reason of invasive treatment is not processed. e risk of transmission
of contagious infections to medical offi cials or patients is lower with saliva compared to blood samples.
QCM (Quartz Crystal Microbalance) which is a sensing device to be able to detect total mass-change on sensor
surface with 0.5 ng/cm2 sensitivity by the alteration of resonance frequency has been used in this study. Sensor
surface has been exposed to (3-Mercaptopropyl)trimethoxysilane (MPS) coating agent to increase the stability
of sensor and provide a proper surface for suffi cient immobilization. Synthetic Probe DNA (pDNA) has been
placed on the sensor surface to make it be able to detect target DNA (tDNA). After pDNA immobilization
and blocking sensor surface with Bovine Serum Albumin (BSA), the surface has been exposed to negative
control and 3 diff erent concentrations of tDNA at least 3 times for each concentration level. Results show
that genetic material in simulated-saliva has been detected without the need of isolation, puri\u1000eb����� cation and
ampli\u1000eb����� cation at 1.2 ng/cm2 (108 copies/mL) of sensitivity level
Genetic Detection in Simulated-Saliva using MPS coatedQCM sensor without DNA Isolation and Amplification
Early diagnosis plays a crucial role in curing the diseases. Especially, nowadays, PCR (Polymerase Chain
Reaction) is used as a Gold Standart for genetically diagnosing illnesses. Alternative ways are being searched
to be used instead of PCR because it takes more time to process, needs more investment, requires fully-armed
laboratory condition which is available and accessible only in developed-countries. An alternative method
which is able to make genetic detection in saliva would have relatively lower infection risks, and patient
comfort would be much higher by the reason of invasive treatment is not processed. e risk of transmission
of contagious infections to medical offi cials or patients is lower with saliva compared to blood samples.
QCM (Quartz Crystal Microbalance) which is a sensing device to be able to detect total mass-change on sensor
surface with 0.5 ng/cm2 sensitivity by the alteration of resonance frequency has been used in this study. Sensor
surface has been exposed to (3-Mercaptopropyl)trimethoxysilane (MPS) coating agent to increase the stability
of sensor and provide a proper surface for suffi cient immobilization. Synthetic Probe DNA (pDNA) has been
placed on the sensor surface to make it be able to detect target DNA (tDNA). After pDNA immobilization
and blocking sensor surface with Bovine Serum Albumin (BSA), the surface has been exposed to negative
control and 3 diff erent concentrations of tDNA at least 3 times for each concentration level. Results show
that genetic material in simulated-saliva has been detected without the need of isolation, puri\u1000eb����� cation and
ampli\u1000eb����� cation at 1.2 ng/cm2 (108 copies/mL) of sensitivity level
The effect of (3-Mercaptopropyl)trimethoxysilane (MPS) coating on the genetic detection performance of quartz crystal microbalance-dissipation (QCM-D) biosensor: novel Intact double-layered surface modification on QCM-D
Biosensor's ability depends on both platform performance and surface modification. In mass sensitive biosensors, a stable coating with molecular structural dynamism increases sensitivity. Also, its orientation and smoothness directly affect the performance of cross-linker molecules and receptors. In this study, the performance of intact double-layered (ID) coating used on QCM-D for the first time in DNA detection of MPS was investigated. The sensitivity of the QCM-D with ID-MPS was compared to the uncoated. At the concentrations of 10(10) and 10(13) copies/mL, the method with ID-MPS was approximately 6.4 times more sensitive. A shift of 1.22(+/- 0.42) Hz was observed at the detection limit at 10(8) copies/mL, while it was 0.19(+/- 0.05) Hz in the negative control. Thus, a signal-to-noise-ratio greater than 3, which is a measure of signal quality in biosensors, was exceeded with a ratio of 3.33, and it was shown that the detection signal at the sensitivity limit was sufficient. In addition, the two methods were compared visually with atomic force microscopy in terms of the amount and distribution of captured molecules, and it was determined that the ID-MPS was more efficient