HEALTHY WORK IN THE AGEING EUROPE

Workplace health promotion (WHP) has been defined as the combined efforts of employers, employees and society to improve the health and well-being of people at work. This is achieved through a combination of: improving the work organization and the working environment , promoting the active participation of employees in health activities, encouraging personal development. In our country, this subject is still unpopular, and organized work on introduction and implementation of already existing directives of ENWHP still does not exist. As a result, the competitiveness of the European Union during the next few decades will depend on the contribution of older workers, especially in comparison with North America and Asia. The general aim, therefore, is to extend workability and health up to a higher age. The most important force for change is the workplace. There are different action plans and a host of tools with which the health, qualifications, motivation and therefore the work ability and employability of a company’s older workers both now and in the future can be fostered

A novel spi1 mutation in a patient with agammaglobulinemia

Agammaglobulinemia is a primary immunodeficiency characterized by a low number or absence of mature B lymphocytes and consequently by immunoglobulin deficiency. In 2021, six patients with pathogenic variants in SPI1 gene associated with agammaglobulinemia type 10 (PU.MA) were described for the first time. This gene encodes the pioneer transcription factor PU.1, which plays an important role in the differentiation of B lymphocytes, monocytes, and conventional dendritic cells. Here we present a female patient with a novel mutation in SPI1 gene which has not been previously found in patients with PU.MA. Case description: A 37-year-old female patient with frequent middle ear infections in early childhood was diagnosed with agammaglobulinemia at the age of 15 when she started immunoglobulin replacement therapy (IgRT). One year later, an allogeneic hematopoietic stem cell transplant from a healthy sibling donor was performed. Unfortunately, chimerism analysis found no DNA material from the donor in the patient's blood, suggesting graft rejection, so she remained dependent on antibody replacement therapy. Years later, she was diagnosed with protein-losing enteropathy, and despite escalating doses of IgRT, IgG levels remained low. Subsequently, the patient developed persistent COVID -19 viremia and bacterial meningoencephalitis. Clinical exome sequencing using the TruSight (Illumina) panel was performed and in comparision with the human reference genome (hg19), has revealed a heterozygous mutation in exon 4 of the SPI1 gene. This mutation is characterized by the insertion of 2 nucleotides (c.441dup), a reading frame shift, and the insertion of a premature stop codon. According to the American College of Medical Genetics and Genomics, this mutation is described as a likely pathogenic-class 2 (PVS1_Very Strong). Conclusion: From analysis of previous literature, we concluded that the mutant sequence in exon 4 encodes the PEST region of the pioneer transcription factor PU.1, which is responsible for interaction with other transcription factors. Immunophenotyping of peripheral blood cells did not reveal CD19+ B cells, suggesting that a differentiation arrest may have developed between the prepro-B and pro-B stages, where there is a high requirement for PU.1 activity. Nextgeneration sequencing can be a very useful tool to uncover the causes of rare primary immunodeficiencies, but further analysis is needed to explain the relationship between patient genotype and clinical presentation

Role of Marketing and Social Networks in Improving Business Effectiveness

Modern technologies offer many opportunities to companies everywhere in the world. Marketing’s and manager’s job is to get to know all those possibilities, to know their posivite and negative side, put it in context and use it in marketing strategy in order to upgrade business of the company, improve sales effectiveness, and finally, raise revenues and value for all stakeholders. This paper contains data on using social networks and technology in advance in company actions, so informations are firstly collected using Facebook, Twitter, Instagram, Google+, Pinterest, LinkedIn, and other social platforms, and the same networks are used to spread informations about business and products, along with feedback used to correct future actions. Informations gathered can be extremely useful, but some can be recognized as unneeded. The main goal of this paper is to show how social networks influence modern businesses and how they can be used as a part of marketing mix in order to improve business effectiveness

On variable Wiener index

1279-1282A variable Wiener index vW, applicable only to acyclic structures, is defined as Σ [n1(e) n2(e)]λ. For the optimal exponent, that value of λ should be selected which gives the smallest standard error of estimate, s, in the structure-property modeling. As a test case, the structure-boiling point modeling of isomeric octanes is used. It is found that the optimal exponent is λ=1/3 which gives a two-parameter model with the lowest value of s (0.72°C) of all tested models. Close to this model is a two-parameter model based on the variable Wiener index with exponent λ=-1/3 (s=0.83°C). Among the published models, only four- and five-parameter models, based on the overall connectivity indices, produce lower values of s (0.71 °C, 0.55°C)